Cytokeratin 8 antibody
Principal name
Cytokeratin 8 antibody
Alternative names for Cytokeratin 8 antibody
KRT8, CYK8, Cytokeratin-8, CK8, Keratin-8, K8, Cytokeratin endo A, Keratin, type II cytoskeletal 8
Ncbi ID
NP_002264.1, NP_002264, 9606, NP_061883, NP_001028782.1, NM_002273, NM_031170
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Enzyme Immunoassay (E), ELISA (detection) (E(detection))
Background of Cytokeratin 8 antibody
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual cytokeratin polypeptides are numbered 1 to 20.
The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium.
Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
General readings
1. Ding Y et al. Proteomic analysis of colonic mucosa in a rat model of irritable bowel syndrome. Proteomics 10:2620-2630 (2010). WB ; Rat . PubMed: 20461717)
2. Yocum AK et al. Coupled global and targeted proteomics of human embryonic stem cells during induced differentiation. Mol Cell Proteomics 7:750-67 (2008). PubMed: 18304949
Detection of cytokeratin 8 in HeLa cell lysate by mouse monoclonal antibody C-51.
Formalin-Fixed, Paraffin-Embedded Human prostate stained with Cytokeratin 8 antibody Cat.-No SM3079P at 20 µg/ml after heat-induced antigen retrieval.
Immunohistochemistry on Paraffin Section of Human colon
Immunohistochemistry on Frozen Section of Human colon
Immunofluorescence staining of a 7 days old Zebrafish embryo.
KRT8 polyclonal antibody (A01), Lot # 050921JC01. Western Blot analysis of KRT8 expression in human ovary cancer.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 8 antibody.
Immunofluorescence staining of methanol-fixed HeLa cells using Keratin 8 antibody (Red).
Western Blot analysis of extracts from HUVEC and 293 cells using Keratin 9 antibody
KRT8 MaxPab polyclonal antibody. Western Blot analysis of KRT8 expression in human placenta.
Western Blot analysis of KRT8 expression in transfected 293T cell line (H00003856-T01) by KRT8 MaxPab polyclonal antibody.Lane 1: KRT8 transfected lysate(53.13 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry (IHC) analyzes of Keratin 8 antibody (Cat.-No.: AP06205PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of Cytokeratin 8 antibody (Cat.-No.: AP06205PU-N) at 1/500 dilution
Lane1: Hela whole cell lysate
Lane2: NIH-3T3 whole cell lysate
Lane3: H9C2 whole cell lysate
Lane1: Hela whole cell lysate
Lane2: NIH-3T3 whole cell lysate
Lane3: H9C2 whole cell lysate
Immunohistochemistry (IHC)analysis of Keratin 8 antibody (Cat.-No.: AP06206PU-N) in paraffin-embedded human breast carcinoma tissue
Western blot (WB) analysis of Cytokeratin 8 antibody at 1/500 dilution
Lane1: DLD whole cell lysate
Lane2: Mouse muscle tissue lysate
Lane3: Rat muscle tissue lysate
Lane1: DLD whole cell lysate
Lane2: Mouse muscle tissue lysate
Lane3: Rat muscle tissue lysate
Immunohistochemistry (IHC) analyzes of K8 antibody (Cat.-No.: AP06531PU-N) in paraffin-embedded human colon carcinomatissue.
Western blot (WB) analysis of K8 antibody (Cat.-No.: AP06531PU-N) in extracts from hela cells.
Kidney: Formalin-Fixed Paraffin Embedded (FFPE)
Western Blot analysis of KRT8 expression in transfected 293T cell line by KRT8 monoclonal antibody (M02), clone 3E3.Lane 1: KRT8 transfected lysate(53.7 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged KRT8 is approximately 1ng/ml as a capture antibody.
KRT8 MaxPab polyclonal antibody. Western Blot analysis of KRT8 expression in human placenta.
Western Blot analysis of KRT8 expression in transfected 293T cell line (H00003856-T01) by KRT8 MaxPab polyclonal antibody.Lane 1: KRT8 transfected lysate(53.13 KDa).Lane 2: Non-transfected lysate.
KRT8 MaxPab polyclonal antibody. Western Blot analysis of KRT8 expression in human liver.
Western Blot analysis of KRT8 expression in transfected 293T cell line (H00003856-T02) by KRT8 MaxPab polyclonal antibody.Lane 1: KRT8 transfected lysate(53.13 KDa).Lane 2: Non-transfected lysate.
KRT8 MaxPab polyclonal antibody. Western Blot analysis of KRT8 expression in human liver.
Western Blot analysis of KRT8 expression in transfected 293T cell line (H00003856-T02) by KRT8 MaxPab polyclonal antibody.Lane 1: KRT8 transfected lysate(53.13 KDa).Lane 2: Non-transfected lysate.
Human Kidney; Rabbit Anti-KRT8 Antibody. Catalog Number: ARP42028. Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; KRT8 Antibody (ARP42028_P050) in Human Kidney cells using Immunohistochemistry
Human Intestine; Small intestine; KRT8 Antibody (ARP42028_P050) in Human Intestine cells using Immunohistochemistry
Human; Sample Type: Human Triple Negative Breast Cancer XenograftsPrimary Dilution: 1:100Secondary (Anti-Rabbit 594) Dilution: 1:200; KRT8 Antibody (ARP42028_P050) in Human cells using Immunohistochemistry
Human Lung; . Rabbit Anti-KRT8 Antibody. . Catalog Number:. ARP42028 . Paraffin Embedded Tissue: Human alveolar cell . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X
Human Jurkat; WB Suggested Anti-KRT8 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; KRT8 Antibody (ARP42028_P050) in Human Jurkat cells using Western Blot
Human Small intestine; Immunohistochemistry with Small intestine tissue at an antibody concentration of 5ug/ml using anti-KRT8 antibody (ARP42029_P050); KRT8 antibody - middle region (ARP42029_P050) in Human Small intestine cells using Immunohistochemistry
Hum. Adult Placenta; Host:Rabbit. Target Name:KRT8. Sample Tissue:Human Adult Placenta. Antibody Dilution: 1.0ug/ml.; KRT8 antibody - middle region (ARP42029_P050) in Hum. Adult Placenta cells using Western Blot
Hum. Fetal Heart; Host:Rabbit. Target Name:KRT8. Sample Tissue:Human Fetal Heart. Antibody Dilution: 1.0ug/ml.; KRT8 antibody - middle region (ARP42029_P050) in Hum. Fetal Heart cells using Western Blot
Human MCF7; Host:Rabbit. Target Name:KRT8. Sample Tissue:Human MCF7. Antibody Dilution: 1.0ug/ml. KRT8 is supported by BioGPS gene expression data to be expressed in MCF7.; KRT8 antibody - middle region (ARP42029_P050) in Human MCF7 cells using Western Blot
Human HepG2; WB Suggested Anti-KRT8 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate. KRT8 is supported by BioGPS gene expression data to be expressed in HepG2.; KRT8 antibody - middle region (ARP42029_P050) in Human HepG2 cells using Western Blot
Hum. Fetal Lung; Host:Rabbit. Target Name:KRT8. Sample Tissue:Human Fetal Lung. Antibody Dilution: 1.0ug/ml.; KRT8 antibody - middle region (ARP42029_P050) in Hum. Fetal Lung cells using Western Blot
AP15623PU Cytokeratin-8 antibody staining of Human Colon.
Human lung stained with Anti-Keratin 8 antibody
Western Blot analysis of HeLa cell lysate with keratin 8 antibody
Western Blot analysis of KRT8 expression in transfected 293T cell line (H00003856-T02) by KRT8 MaxPab polyclonal antibody.Lane 1: KRT8 transfected lysate(53.70 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical staining of KRT8 on formalin fixed, paraffin embedded human lung with KRT8 polyclonal antibody.
Immunohistochemical staining of KRT8 on formalin fixed, paraffin embedded human lung cancer with KRT8 polyclonal antibody ( Cat # PAB12326 ).
Immunohistochemical staining of KRT8 on formalin fixed, paraffin embedded human stomach cancer with KRT8 polyclonal antibody ( Cat # PAB12326 ).
Western Blot analysis of KRT8 expression from cell extracts with KRT8 polyclonal antibody ( Cat # PAB12326 ).Lane 1 : HeLa whole cell lysate.Lane 2 : HT1080 whole cell lysate.Lane 3 : Colo 320 whole cell lysate.
Cell Cytospin of the HCC38 breast carcinoma cell line was incubated with KRT8 monoclonal antibody, clone SB37b ( Cat # MAB5629 ). Positive cells were viewed by fluorescence microscopy.
Figure 1. Immunofluorescence staining of methanol-fixed HeLa cells using Keratin 8 (Phospho-Ser74) Antibody (#AP09459PU, Red).
Figure 2. Western blot analysis of various cell extracts using Keratin 8 (Phospho-Ser74) Antibody (#AP09459PU)
Human breast ductal carcinoma stained with anti-Cytokeratin 8 antibody AM20269PU
Cytokeratin 8 Polyclonal Antibody
Cytokeratin 8 Polyclonal Antibody
Formalin-Fixed, Paraffin-Embedded Human breast cancer tissue (A, 4 μm), Human stomach adenocarcinoma (B, 4 μm) and Human colon adenocarcinoma (C, 4 μm), stained with anti-Cytokeratin 8 antibody (DB 098) shows strong positive cytoplasmic immunostaining of neoplastic cells (A), strong positive cytoplasmic immunostaining of tumor cells (B, C). Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph.D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic.
Formalin-Fixed, Paraffin-Embedded Human breast cancer tissue (A, 4 μm), Human stomach adenocarcinoma (B, 4 μm) and Human colon adenocarcinoma (C, 4 μm), stained with anti-Cytokeratin 8 antibody (DB 098) shows strong positive cytoplasmic immunostaining of neoplastic cells (A), strong positive cytoplasmic immunostaining of tumor cells (B, C). Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph.D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic.
Formalin-Fixed, Paraffin-Embedded Human breast cancer tissue (A, 4 μm), Human stomach adenocarcinoma (B, 4 μm) and Human colon adenocarcinoma (C, 4 μm), stained with anti-Cytokeratin 8 antibody (DB 098) shows strong positive cytoplasmic immunostaining of neoplastic cells (A), strong positive cytoplasmic immunostaining of tumor cells (B, C). Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph.D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic.
Formalin-Fixed, Paraffin-Embedded Human breast cancer tissue (A, 4 μm), Human stomach adenocarcinoma (B, 4 μm) and Human colon adenocarcinoma (C, 4 μm), stained with anti-Cytokeratin 8 antibody (DB 098) shows strong positive cytoplasmic immunostaining of neoplastic cells (A), strong positive cytoplasmic immunostaining of tumor cells (B, C). Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph.D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic.
Formalin-Fixed, Paraffin-Embedded Human ovarian carcinoma stained with Cytokeratin-7/8 antibody Cat.-No. AM10104SU-N using peroxidase conjugate and AEC chromogen. Note the cytoplasmic staining of tumor cells.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A), lung cancer (B) and ovarian cancer tissue (C), showing membrane and cytoplasmic localization with DAB staining using Cytokeratin 8 antibody Cat.-No AM06284SU-N
Immunohistochemical analysis of paraffin-embedded human Kidney tissues using Cytokeratin 8 antibody Cat.-No AM06284SU-N
Confocal Immunofluorescence analysis of methanol-fixed ECA109 cells (left) and HepG2 cells (right) using Cytokeratin 8 antibody Cat.-No AM06284SU-N (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
Western blot analysis using Cytokeratin 8 antibody Cat.-No AM06284SU-N against A549 (1), Hela (2), MCF-7 (3), A431 (4) and HepG2 (5) cell lysate.
The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT8 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunofluorescence staining of a 7 days old Zebrafish embryo
Formalin-paraffin colon carcinoma stained with Cytokeratin 8 Antibody Cat.-No AM50127PU (Clone SPM538).
Formalin-paraffin colon carcinoma stained with Cytokeratin 8 Antibody Cat.-No AM50128PU (Clone SPM192).
Formalin-paraffin colon carcinoma stained with Cytokeratin 8 Antibody Cat.-No AM50258PU (Clone K8/383).
Formalin-paraffin colon carcinoma stained with Cytokeratin 8 Antibody Cat.-No AM50259PU (Clone H1+TS1).
Formalin-paraffin colon carcinoma stained with Cytokeratin 8 Antibody Cat.-No AM50260PU (Clone K8.8)
Formalin-paraffin colon carcinoma stained with Cytokeratin 8/18 Antibody Cat.-No AM50261PU (Clone K8.8+DC10).
Formalin-Paraffin colon carcinoma stained with Cytokeratin 8 Antibody AM33362PU (Clone TS1).
Western blot analysis of Cytokeratin 8 (pS73) expression in HeLa (A); NIH3T3 (B); H9C2 (C) whole cell lysates.
Immunohistochemical analysis of Cytokeratin 8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Cytokeratin 8 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Cytokeratin 8 expression in HeLa (A); NIH3T3 (B); H9C2 (C); MCF7 (D) whole cell lysates.
Immunohistochemical analysis of Cytokeratin 8 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Cytokeratin 8 staining in DLD cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Cytokeratin 8 expression in DLD (A); mouse muscle (B); rat muscle (C) whole cell lysates.
Immunohistochemical analysis of Cytokeratin 8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Cytokeratin 8 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Cytokeratin 8 expression in HeLa (A); A431 (B) whole cell lysates.
Anti-KRT8 mouse monoclonal antibody (TA500020) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT8(RC209570).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8 (RC209570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT8 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.
Immunofluorescent staining of HeLa cells using anti-KRT8 mouse monoclonal antibody (TA500021).
Anti-KRT8 mouse monoclonal antibody (TA500021) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT8(RC209570).
Immunofluorescent staining of A549 cells using anti-KRT8 mouse monoclonal antibody (TA500021).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8 (RC209570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT8 monoclonal antibody.
Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-KRT8 monoclonal antibody at 1:1000 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KRT8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500022)
Immunofluorescent staining of A549 cells using anti-KRT8 mouse monoclonal antibody (TA500022).
Anti-KRT8 mouse monoclonal antibody (TA500022) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT8(RC209570).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT8 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8 (RC209570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8.
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-KRT8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500216)
Immunofluorescent staining of A549 cells using anti-KRT8 mouse monoclonal antibody (TA500216).
Anti-KRT8 mouse monoclonal antibody (TA500216) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT8(RC209570).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8 (RC209570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT8 monoclonal antibody.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-KRT8 mouse monoclonal antibody (Clone UMAB1). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-KRT8 (Clone UMAB1) very specifically recognizes KRT8 antigen on OriGene protein microarray chip.
Western blot analysis of extracts from SKOV3 cells, using Keratin 8 antibody (#TA313111).The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 8 antibody (#TA313111).The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using Cytokeratin 8 (Ab-431) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Cytokeratin 8 (Ab-431) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using Cytokeratin 8 (Ab-431) antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of Keratin 8 expression in EGF treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of extracts of various cell lines, using KRT8 antibody.
Immunohistochemistry of paraffin-embedded human breast cancer using KRT8 antibody at dilution of 1:200 (400x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using KRT8 antibody at dilution of 1:200 (400x lens).
Immunofluorescence analysis of HeLa cell using KRT8 antibody. Blue: DAPI for nuclear staining.
Immunohistochemistry staining of Paraffin Prostate, salivary gland tissue by Cytokeratin 8&18 antibody (dilution: 1:100 - 1:500; visualization of staining: Cytoplasmic)
Western blot analysis on 293 cell lysate using Keratin 8 Antibody
Western blot analysis of Cytokeratin 8 expression in Hela (A); mouse brain (B) whole cell lysates.
Immunohistochemical analysis of Cytokeratin 8 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Human Breast Ductal Carcinoma stained with anti-Keratin 8 antibody.
Human Breast stained with anti-Keratin 8 antibody.
Human Lung Squamous Cell Carcinoma stained with anti-Keratin 8 antibody.
Human Placenta stained with anti-Keratin 8 antibody.
Human Thyroid stained with anti-Keratin 8 antibody.
Human Breast Ductal Carcinoma stained with anti-Keratin 8 antibody.
Human Bladder stained with anti-Keratin 8 antibody.
Human Bladder Transitional Cell Carcinoma stained with anti-Keratin 8 antibody.
Human Stomach stained with anti-Keratin 8 antibody.
Human Stomach Adenocarcinoma stained with anti-Keratin 8 antibody.
Human Prostate stained with anti-Keratin 8 antibody.
Human Prostate Adenocarcinoma stained with anti-Keratin 8 antibody.
Human Lung stained with anti-Keratin 8 antibody.
Western Blot analysis of HeLa cell lysate with keratin 8 antibody
Human Lung stained with anti-Keratin 8 antibody.
Human Colon Carcinoma stained with anti-Keratin 8 antibody.
The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (KRT8 Antibody) at dilution 1/40. (Original magnification: ×200)
The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (KRT8 Antibody) at dilution 1/40. (Original magnification: ×200)
Western blot analysis of extracts from HepG2 cells, using Phospho-KRT8-S74 antibody.
Western blot analysis of Keratin 8 phosphorylation expression in EGF treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| DM189 | Cytokeratin 8 (+ KRT18) antibody |
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Mouse | IgG2a | 5D3 | Ascites | Hu | C, P, WB |
1 ml /
€360.00
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| Acris Antibodies GmbH | |||||||
Published recently! |
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| SM3079P | Cytokeratin 8 antibody |
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 |
Mouse | IgG1 | C-43 | Aff - Purified | Bov, Hu, Por, Rb, Sh | ICC/IF, IP, P, WB |
0.1 mg /
€210.00
|
| Acris Antibodies GmbH | |||||||
Published recently! |
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| UM500001 | Cytokeratin 8 antibody |
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| CK8 mouse monoclonal antibody, clone UMAB1 | |||||||
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Mouse | IgG1 | UMAB1 | Purified | Hu, Mky | ICC/IF, P, WB |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
 +6 additional images |
Highly validated antibody!
|
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| UM570001 | Cytokeratin 8 antibody |
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| CK8 mouse monoclonal antibody, clone UMAB1 | |||||||
|
Mouse | IgG1 | UMAB1 | Purified | Hu, Mky | ICC/IF, P, WB |
30 µl /
€140.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
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| TA326893 | Cytokeratin 8 antibody |
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| KRT8 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€325.00
|
||
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA353912 | Cytokeratin 8 antibody |
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| Rabbit Monoclonal anti-Keratin 8 Antibody | |||||||
 |
Rabbit | IgG | SP102 | Purified | Hu | P |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +12 additional images |
Highly validated antibody! |
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| TA500020 | Cytokeratin 8 antibody |
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| CK8 (Keratin 8) mouse monoclonal antibody, clone OTI1B9 (formerly 1B9) | |||||||
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Mouse | IgG2a | OTI1B9 | Purified | Can, Hu, Mky | ICC/IF, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA500021 | Cytokeratin 8 antibody |
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| CK8 (Keratin 8) mouse monoclonal antibody, clone OTI1B12 (formerly 1B12) | |||||||
 |
Mouse | IgG2b | OTI1B12 | Purified | Can, Hu, Mky, Rt | ICC/IF, P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +15 additional images |
Highly validated antibody!
|
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| TA500022 | Cytokeratin 8 antibody |
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| CK8 (Keratin 8) mouse monoclonal antibody, clone OTI2E4 (formerly 2E4) | |||||||
 |
Mouse | IgG1 | OTI2E4 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA500216 | Cytokeratin 8 antibody |
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| CK8 (Keratin 8) mouse monoclonal antibody, clone OTI4D12 (formerly 4D12) | |||||||
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Mouse | IgG2a | OTI4D12 | Purified | Can, Hu, Mky | ICC/IF, P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| AM06284SU-N | Cytokeratin 8 antibody |
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 |
Mouse | IgG1 | 8A5D12 | Ascites | Hu | E, ICC/IF, P, WB |
0.1 ml /
€440.00
|
| Acris Antibodies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
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| DB 098-0.1 | Cytokeratin 8 (C-term) antibody |
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Rabbit | IgG | R15-K | Aff - Purified | Hu | C, P |
0.1 ml /
€250.00
|
| DB Biotech, spol. s r.o. | |||||||
Acris recommends this product for Immunohistochemistry.
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| DB 098-0.2 | Cytokeratin 8 (C-term) antibody |
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Rabbit | IgG | R15-K | Aff - Purified | Hu | C, P |
0.2 ml /
€330.00
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| DB Biotech, spol. s r.o. | |||||||
Acris recommends this product for Immunohistochemistry.
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| DB 098-0.5 | Cytokeratin 8 (C-term) antibody |
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Rabbit | IgG | R15-K | Aff - Purified | Hu | C, P |
0.5 ml /
€410.00
|
| DB Biotech, spol. s r.o. | |||||||
Acris recommends this product for Immunohistochemistry.
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| DB 098-1 | Cytokeratin 8 (C-term) antibody |
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 |
Rabbit | IgG | R15-K | Aff - Purified | Hu | C, P |
1 ml /
€630.00
|
| DB Biotech, spol. s r.o. | |||||||
Acris recommends this product for Immunohistochemistry.
|
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| AM33362PU-N | Cytokeratin 8 antibody |
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 |
Mouse | IgG1 | TS1 | Purified | Hu | C, F, ICC/IF, IP, P, WB |
0.2 mg /
€410.00
|
| Acris Antibodies GmbH | |||||||
Acris recommends this product for Immunohistochemistry. |
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| AM33362PU-S | Cytokeratin 8 antibody |
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|
Mouse | IgG1 | TS1 | Purified | Hu | C, F, ICC/IF, IP, P, WB |
0.1 mg /
€320.00
|
| Acris Antibodies GmbH | |||||||
Acris recommends this product for Immunohistochemistry. |
|||||||
| AM33362PU-T | Cytokeratin 8 antibody |
||||||
|
Mouse | IgG1 | TS1 | Purified | Hu | C, F, ICC/IF, IP, P, WB |
20 µg /
€180.00
|
| Acris Antibodies GmbH | |||||||
Acris recommends this product for Immunohistochemistry. |
|||||||
| AM50127PU-N | Cytokeratin 8 antibody |
||||||
 |
Mouse | IgG1 | SPM538 | Purified | Hu, Rt, Ze | C, F, ICC/IF, IP, P, WB |
0.2 mg /
€410.00
|
| Acris Antibodies GmbH | |||||||
Acris recommends this product for Immunohistochemistry. |
|||||||
| AM50127PU-S | Cytokeratin 8 antibody |
||||||
|
Mouse | IgG1 | SPM538 | Purified | Hu, Rt, Ze | C, F, ICC/IF, IP, P, WB |
0.1 mg /
€320.00
|
| Acris Antibodies GmbH | |||||||
Acris recommends this product for Immunohistochemistry. |
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