DAAM1 antibody

Principal name

DAAM1 antibody

Alternative names for DAAM1 antibody

KIAA0666, FLJ41657, DAAM-1, Disheveled-associated activator of morphogenesis 1

SwissProt ID

Q9Y4D1 (Human)

Gene ID

23002 (DAAM1)

Available reactivities

Hu (Human), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of DAAM1 antibody

Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein.Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein. Evidence of alternative splicing has been observed for this gene but the full-length nature of these variants has not been determined.

General readings

Higashi,T., (2008) J. Biol. Chem. 283 (13), 8746-8755

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DAAM1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between RHOA and DAAM1 HeLa cells were stained with anti-RHOA rabbit purified polyclonal 1:1200 and anti-DAAM1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 4H3 Purified Hu, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DAAM1 antibody

Western Blot analysis of DAAM1 expression in transfected 293T cell line by DAAM1 monoclonal antibody (M05), clone 5D3.

Lane 1: DAAM1 transfected lysate(122 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 5D3 Purified Hu, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DAAM1 antibody

WB Suggested Anti-DAAM1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 293T cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

DAAM1 (N-term) antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with DAAM1 Antibody (N-term) (Cat.#AP12338PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DAAM1 (C-term) antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with DAAM1 Antibody (C-term) (Cat.#AP12339PU-N) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DAAM1 antibody

DAAM1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

DAAM1 antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with DAAM1 polyclonal antibody ( Cat # PAB1951 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

DAAM1 antibody

Human 293T; WB Suggested Anti-DAAM1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate; DAAM1 antibody - middle region (ARP55131_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

DAAM1 antibody

Western Blot: DAAM1 Antibody [NBP1-53127] - This Anti-DAAM1 antibody was used in Western Blot of 293T tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

DAAM1 antibody

Immunohistochemistry-Paraffin: DAAM1 Antibody [NBP1-81492] - Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DAAM1

DAAM1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

DAAM1

DAAM1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

DAAM1

DAAM1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

DAAM1 HEK293 Cell Transient Overexpression Lysate (Non-Denatured)

DAAM1 HEK293 Cell Transient Overexpression Lysate (Non-Denatured)  
0.1 mg / €270.00
  Abnova Taiwan Corp.

DAAM1 overexpression lysate

DAAM1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

15 Item(s)

per page
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