DAG kinase epsilon antibody

Principal name

DAG kinase epsilon antibody

Alternative names for DAG kinase epsilon antibody

Diacylglycerol kinase epsilon, DGKE, DAGK5, Diglyceride kinase epsilon, DGK-epsilon

SwissProt ID

P52429 (Human), Q9R1C6 (Mouse)

Gene ID

8526 (DGKE), 56077 (Dgke)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of DAG kinase epsilon antibody

Diacylglycerol (DAG) influences numerous cell signaling cascades by functioning as an intracellular, allosteric activator of protein kinase C (PKC), and as a potent activator of guanine nucleotide exchange factors. In order to maintain cellular homeostasis, intracellular DAG levels are tightly regulated by diacylglycerol kinases (DGKs, DAGKs), which phosphorylate DAG to phosphatidic acid, thus removing DAG. Human DGK-α (80 kDa), -β (90 kDa), and -γ (90 kDa) have calcium-binding EF-hand motifs at their N termini and are classified as type I DGKs. Human DGK-δ (130 kDa) and DGK-?(130 kDa) contain N-terminal pleckstrin homology (PH) domains and are classified as type II. Human DGK-ε (64 kDa) contains no identifiable regulatory domains and is classified as a type III DGK. Human DGK-ζ (104 kDa) and -iota (130 kDa) possess C-terminal ankyrin repeats and are classified as type IV DGKs. Human DGK-θ (110 kDa) contains 3 cysteine-rich domains and a PH domain and is classified as a type V DGK.

General readings

Epand,R.M., (2007) Biochemistry 46 (49), 14225-14231

16 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DAG kinase epsilon antibody

  Rabbit polyclonal anti-DGKE antibody  
Western blot analysis of extracts from K562 cells, using DGKE antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DAG kinase epsilon antibody

  Rabbit Polyclonal Anti-DGKE Antibody  
WB Suggested Anti-DGKE Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human brain Rabbit IgG Purified Hu, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

DAG kinase epsilon antibody

Western blot analysis of DGK-ε antibody (Cat.-No.: AP20421PU-N) in extracts from K562 cells. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

DAG kinase epsilon antibody

Western blot analysis of extracts from K562 cells, using DGKE Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

DAG kinase epsilon antibody

Western blot analysis of extracts from K562 cells, using DGKE Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

DGKE - human gene knockout kit via CRISPR

DGKE - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

DGKE - human gene knockout kit via CRISPR

DGKE - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

DGKE - human gene knockout kit via CRISPR

DGKE - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

DGKE - human gene knockout kit via CRISPR

DGKE - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dgke - mouse gene knockout kit via CRISPR

Dgke - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Dgke - mouse gene knockout kit via CRISPR

Dgke - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dgke - mouse gene knockout kit via CRISPR

Dgke - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dgke - mouse gene knockout kit via CRISPR

Dgke - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DAG kinase epsilon

  Recombinant protein of human diacylglycerol kinase, epsilon 64kDa (DGKE)  
DAG kinase epsilon Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, DGKE (mGFP-tagged) - Human diacylglycerol kinase, epsilon 64kDa (DGKE), 200 uL, >10^7 TU/mL

Lenti ORF particles, DGKE (mGFP-tagged) - Human diacylglycerol kinase, epsilon 64kDa (DGKE), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, DGKE (Myc-DDK tagged) - Human diacylglycerol kinase, epsilon 64kDa (DGKE) , 200 uL, >10^7 TU/mL

Lenti ORF particles, DGKE (Myc-DDK tagged) - Human diacylglycerol kinase, epsilon 64kDa (DGKE) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

16 Item(s)

per page
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