DAG kinase zeta antibody
Principal name
DAG kinase zeta antibody
Alternative names for DAG kinase zeta antibody
Diacylglycerol kinase zeta, DGKZ, DAGK6, Diglyceride kinase zeta, DGK-zeta
Ncbi ID
9606, NP_001099010, NP_001099010.1, NM_003646, NP_003637, NM_031143, NM_201533, NM_201532, NM_001105540
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)
Background of DAG kinase zeta antibody
- Kit Component:
- - KN205327G1, DGKZ gRNA vector 1 in pCas-Guide vector
- KN205327G2, DGKZ gRNA vector 2 in pCas-Guide vector
- KN205327D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Formalin-fixed and paraffin-embedded human cancer tissue reacted with DGKZ polyclonal antibody ( Cat # PAB3265 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
The DGKZ polyclonal antibody ( Cat # PAB3265 ) is used in Western blot to detect DGKZ in Ramos cell lysate.
Western Blot analysis of DGKZ expression in transfected 293T cell line (H00008525-T01) by DGKZ MaxPab polyclonal antibody.Lane 1: DGKZ transfected lysate(102.19 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of DGKZ expression in transfected 293T cell line (H00008525-T01) by DGKZ MaxPab polyclonal antibody.Lane 1: DGKZ transfected lysate(102.19 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of DGKZ expression in transfected 293T cell line (H00008525-T02) by DGKZ MaxPab polyclonal antibody.Lane 1: DGKZ transfected lysate(104.10 KDa).Lane 2: Non-transfected lysate.
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
DGKZ Antibody (D1004) (Cat. #TA302108) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the DGKZ antibody detected the DGKZ protein (arrow).
Western blot analysis of DGKZ (arrow) using DGKZ Antibody (C-term) (Cat.#TA302108). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DGKZ gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
SF1 colocalizes with DGK and PA in the nuclei of H295R cells. Cells were plated onto glass coverslips, fixed, permeabilized, and incubated with anti-SF1 and anti-DGKZ (B) for 1 h. Coverslips were washed and incubated with anti-fluorescein isothiocyanate and antirhodamine, and immunofluorescence was detected by confocal microscopy.
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using (DGKZ Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (DGKZ Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302108 | DAG kinase zeta (C-term) antibody |
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| Rabbit Polyclonal Antibody against DGKZ (C-term) | |||||||
 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA350918 | DAG kinase zeta antibody |
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| Rabbit Polyclonal Anti-DGKZ Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AP32262PU-N | DAG kinase zeta antibody |
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 |
Rabbit | IgG | Aff - Purified | Hu | E, P |
50 µl /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN205327 | DGKZ - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205327BN | DGKZ - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205327LP | DGKZ - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205327RB | DGKZ - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305327 | DAG kinase zeta (transcript variant 2) |
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| Recombinant protein of human diacylglycerol kinase, zeta 104kDa (DGKZ), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY418529 | DGKZ overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR212478L2V | Lenti ORF particles, Dgkz (GFP-tagged ORF) - Rat diacylglycerol kinase zeta (Dgkz), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,780.00
|
|
| OriGene Technologies, Inc. | ||
| RR212478L1V | Lenti ORF particles, Dgkz (Myc-DDK-tagged ORF) - Rat diacylglycerol kinase zeta (Dgkz), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,610.00
|
|
| OriGene Technologies, Inc. | ||
