DAGLA antibody
Principal name
DAGLA antibody
Alternative names for DAGLA antibody
DGL-alpha, NSDDR, C11orf11, KIAA0659, DAGLALPHA, DAGL, DGLa, Sn1-specific diacylglycerol lipase alpha, Neural stem cell-derived dendrite regulator
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)
Background of DAGLA antibody
- Kit Component:
- - KN214261G1, DAGLA gRNA vector 1 in pCas-Guide vector
- KN214261G2, DAGLA gRNA vector 2 in pCas-Guide vector
- KN214261D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
1. Bisogno T, Howell F, Williams G, Minassi A, Cascio MG, Ligresti A, Matias I, Schiano-Moriello A, Paul P, Williams EJ, Gangadharan U, Hobbs C, Di Marzo V, Doherty P. Cloning of the first sn1-DAG lipases points to the spatial and temporal regulation of endocannabinoid signaling in the brain. J Cell Biol. 2003 Nov 10;163(3):463-8.
PMID: 14610053
DAGLA polyclonal antibody ( Cat # PAB11515 ) ( 0.3 µg/ml ) staining of human liver lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence.
Human Spleen (formalin-fixed, paraffin-embedded) stained with DAGLA antibody AP22525PU-N at 5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Pancreas (formalin-fixed, paraffin-embedded) stained with DAGLA antibody AP22525PU-N at 5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Breast (formalin-fixed, paraffin-embedded) stained with DAGLA antibody AP22525PU-N at 5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Antibody (0.3 µg/ml) staining of Human Liver lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Formalin fixed, paraffin embedded human brain tissue stained with DAGLA Antibody Cat.-No AP51181PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DAGLA antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of DAGLA Antibody Cat.-No AP51181PU-N in NCI-H292 cell line lysates (35ug/lane). This demonstrates the DAGLA antibody detected the DAGLA protein (arrow).
Western blot analysis of DAGLA Antibody Cat.-No AP51181PU-N in Mouse heart tissue lysates (35ug/lane).This demonstrates the DAGLA antibody detected the DAGLA protein (arrow).
TA305875 (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP22525PU-N | DAGLA (1031-1042) antibody |
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 |
Goat | Aff - Purified | Bear, Bov, Elephant, Eq, Hst, Hu, Mky, Ms, Por, Prim, Rb, Rt | E, P, WB |
50 µg /
€470.00
|
||
| Acris Antibodies GmbH | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA305875 | DAGLA (C-term) antibody |
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| Goat Anti-DAGLA Antibody | |||||||
 |
Goat | Aff - Purified | Hu | E, P, WB |
0.1 mg /
€325.00
|
||
| OriGene Technologies, Inc. | |||||||
| AP51181PU-N | DAGLA (Center) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu, Ms | P, WB |
0.4 ml /
€370.00
|
|
| Acris Antibodies GmbH | |||||||
| +2 additional images |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN214261 | DAGLA - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN214261BN | DAGLA - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN214261LP | DAGLA - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN214261RB | DAGLA - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC214261L2V | Lenti ORF particles, DAGLA (mGFP-tagged)-Human diacylglycerol lipase, alpha (DAGLA), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,320.00
|
|
| OriGene Technologies, Inc. | ||
| RC214261L1V | Lenti ORF particles, DAGLA (Myc-DDK-tagged)-Human diacylglycerol lipase, alpha (DAGLA), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,180.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR204355L2V | Lenti ORF particles, Dagla (GFP-tagged ORF) - Rat diacylglycerol lipase, alpha (Dagla), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,180.00
|
|
| OriGene Technologies, Inc. | ||
| RR204355L1V | Lenti ORF particles, Dagla (Myc-DDK-tagged ORF) - Rat diacylglycerol lipase, alpha (Dagla), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,968.00
|
|
| OriGene Technologies, Inc. | ||
