DAP10 / HCST antibody

Principal name

DAP10 / HCST antibody

Alternative names for DAP10 / HCST antibody

Hematopoietic cell signal transducer, KAP10, PIK3AP, Transmembrane adapter protein KAP10, DNAX-activation protein 10, Membrane protein DAP10

SwissProt ID

Q1XF11 (Bovin), Q6X9T8 (Rat), Q9GJR5 (Pig), Q9QUJ0 (Mouse), Q9UBK5 (Human)

Gene ID

10870 (HCST), 23900 (Hcst), 474146

Available reactivities

Hu (Human)

Available hosts

Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB)

Background of DAP10 / HCST antibody

Kit Component:
- KN307613G1, Hcst gRNA vector 1 in pCas-Guide vector
- KN307613G2, Hcst gRNA vector 2 in pCas-Guide vector
- KN307613D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

1. Upshaw JL, Arneson LN, Schoon RA, Dick CJ, Billadeau DD, Leibson PJ. NKG2D-mediated signaling requires a DAP10-bound Grb2-Vav1 intermediate and phosphatidylinositol-3-kinase in human natural killer cells. Nat Immunol. 2006 May;7(5):524-32. Epub 2006 Apr 2.
PMID: 16582911

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DAP10 / HCST (C-term) antibody

  Goat Polyclonal Antibody against HCST  
TA303159 (0.2µg/ml) staining of human spleen lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

HCST - human gene knockout kit via CRISPR

HCST - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

HCST - human gene knockout kit via CRISPR

HCST - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

HCST - human gene knockout kit via CRISPR

HCST - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

HCST - human gene knockout kit via CRISPR

HCST - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Hcst - mouse gene knockout kit via CRISPR

Hcst - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Hcst - mouse gene knockout kit via CRISPR

Hcst - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Hcst - mouse gene knockout kit via CRISPR

Hcst - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Hcst - mouse gene knockout kit via CRISPR

Hcst - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DAP10 / HCST (transcript variant 2)

  Recombinant protein of human hematopoietic cell signal transducer (HCST), transcript variant 2  
DAP10 / HCST Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

HCST overexpression lysate

HCST overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

HCST overexpression lysate

HCST overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, HCST (mGFP-tagged) - Human hematopoietic cell signal transducer (HCST), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, HCST (mGFP-tagged) - Human hematopoietic cell signal transducer (HCST), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, HCST (mGFP-tagged) - Human hematopoietic cell signal transducer (HCST), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, HCST (mGFP-tagged) - Human hematopoietic cell signal transducer (HCST), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, HCST (Myc-DDK tagged) - Human hematopoietic cell signal transducer (HCST), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, HCST (Myc-DDK tagged) - Human hematopoietic cell signal transducer (HCST), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Lenti ORF particles, HCST (Myc-DDK tagged) - Human hematopoietic cell signal transducer (HCST), transcript variant 2 , 200 uL, >10^7 TU/mL

Lenti ORF particles, HCST (Myc-DDK tagged) - Human hematopoietic cell signal transducer (HCST), transcript variant 2 , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Hcst (GFP-tagged) - Mouse hematopoietic cell signal transducer (cDNA clone MGC:73601 IMAGE:640698), 200 uL, >10^7 TU/mL

Lenti ORF particles, Hcst (GFP-tagged) - Mouse hematopoietic cell signal transducer (cDNA clone MGC:73601 IMAGE:640698), 200 uL, >10^7 TU/mL  
200 µl / €790.00
  OriGene Technologies, Inc.

Lenti ORF particles, Hcst (GFP-tagged) - Mouse hematopoietic cell signal transducer (Hcst), 200 uL, >10^7 TU/mL

Lenti ORF particles, Hcst (GFP-tagged) - Mouse hematopoietic cell signal transducer (Hcst), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Hcst (Myc-DDK-tagged) - Mouse hematopoietic cell signal transducer (cDNA clone MGC:73601 IMAGE:640698), 200 uL, >10^7 TU/mL

Lenti ORF particles, Hcst (Myc-DDK-tagged) - Mouse hematopoietic cell signal transducer (cDNA clone MGC:73601 IMAGE:640698), 200 uL, >10^7 TU/mL  
200 µl / €700.00
  OriGene Technologies, Inc.

Lenti ORF particles, Hcst (Myc-DDK-tagged) - Mouse hematopoietic cell signal transducer (Hcst), 200 uL, >10^7 TU/mL

Lenti ORF particles, Hcst (Myc-DDK-tagged) - Mouse hematopoietic cell signal transducer (Hcst), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

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