DAP3 / MRPS29 antibody

Principal name

DAP3 / MRPS29 antibody

Alternative names for DAP3 / MRPS29 antibody

Death-associated protein 3, DAP-3, MRP-S29, 28S ribosomal protein S29 mitochondrial, Ionizing radiation resistance conferring protein

SwissProt ID

P51398 (Human), P82922 (Bovin), Q9ER88 (Mouse)

Gene ID

7818 (DAP3), 65111 (Dap3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of DAP3 / MRPS29 antibody

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (med DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kise which carries ankyrin repeats and a death domain (DAP-kise), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5). DAP-3 has an ATP/GTP binding motive (P-loop) and has been isolated from human and mouse, and med as hDAP-3 and mDAP-3 (Death Associated Protein-3), respectively. Sequence alysis revealed an 82% amino acid identity between human and mouse protein. In situ hybridization and northern blot alysis showed an abundant mR expression with a pronounced expression in highly proliferative epithelial compartments. Overexpression of DAP-3 leads to cytochrome c release and induction of cell death. DAP-3 mediated apoptosis was shown to depend on a functiol P-loop. mDAP-3 is localized to the mitochondrial matrix and in contrast to cytochrome c, retained its mitochondrial localization during apoptosis induction.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DAP3 / MRPS29 (C-term) antibody

TA302460 staining (3µg/ml) of HeLa lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DAP3 / MRPS29 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DAP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802927) Mouse IgG1 OTI1B12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DAP3 / MRPS29 antibody

Predicted band size: 46 kDa . Positive control: 293T and Jurkat cell, human lymphoma tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lane1: 293T cell lysate Lane2: Jurkat cell lysate Lane3: Human Lymphoma tissue lysate Lysates: 30 ug per lane Primary antibody: 1/150 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DAP3 / MRPS29 antibody

Predicted band size: 46 kDa . Positive control: Human lymphoma tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 30 ug Primary antibody: 1/150 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DAP3 / MRPS29 antibody

Predicted band size: 45 kDa . Positive control: Hepg2 and Jurkat cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: Hepg2 cell lysate Lane2: Jurkat cell lysate Lysates: 30 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DAP3 / MRPS29 antibody

Predicted band size: 45 kDa . Positive control: Hepg2 and Hela cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: Hepg2 cell lysate Lane2: Hela cell lysate Lysates: 30 ug per lane Primary antibody: 1/900 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DAP3 / MRPS29 antibody

Western Blot: DAP3 Antibody (42C617.1.2) [TA336396] - Analysis of DAP-3 in A) human brain, B) Jurkat and C) NIH 3T3 lysate using this antibody at 5 ug/ml. Mouse IgG 42C617.1.2 Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DAP3 / MRPS29 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-DAP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802787) Mouse IgG1 OTI1A12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DAP3 / MRPS29 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP3 (RC223182, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAP3. Mouse IgG1 OTI2D4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DAP3 / MRPS29 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP3 (RC223182, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAP3. Mouse IgG1 OTI6E11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DAP3 / MRPS29 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP3 (RC223182, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAP3. Mouse IgG1 OTI10B10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DAP3 / MRPS29 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP3 (RC223182, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAP3. Mouse IgG1 OTI3H6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DAP3 / MRPS29 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP3 (RC223182, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAP3. Mouse IgG1 OTI1B5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DAP3 / MRPS29 antibody

DAP3 / MRPS29 Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

DAP3 / MRPS29 antibody

DAP3 / MRPS29 Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

DAP3 / MRPS29 antibody

DAP3 / MRPS29 Mouse IgG2a 3B7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DAP3 / MRPS29 antibody

Western blot analysis of 10 µg of total cell lysate from Jurkat cells with Dap3 polyclonal antibody ( Cat # PAB0169 ) at 1 µg/ml. Rabbit Hu, Ms, Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

DAP3 / MRPS29 antibody

HEK293 overexpressing DAP3 and probed with DAP3 polyclonal antibody ( Cat # PAB6163 ) ( mock transfection in first lane ), tested by Origene. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

DAP3 / MRPS29 (C-term) antibody

Human U937; WB Suggested Anti-DAP3 Antibody. . Titration: 1.0 ug/ml. . Positive Control: U937 Whole Cell; DAP3 antibody - C-terminal region (ARP61638_P050) in Human U937 cells using Western Blot Rabbit Aff - Purified Hu
50 µg / €350.00
  AVIVA Systems Biology

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DAP3 / MRPS29 (transcript variant 1)

DAP3 / MRPS29 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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