DAP3 / MRPS29 antibody

Principal name

DAP3 / MRPS29 antibody

Alternative names for DAP3 / MRPS29 antibody

Death-associated protein 3, DAP-3, MRP-S29, 28S ribosomal protein S29 mitochondrial, Ionizing radiation resistance conferring protein

SwissProt ID

P51398 (Human), P82922 (Bovin), Q9ER88 (Mouse)

Gene ID

7818 (DAP3), 65111 (Dap3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of DAP3 / MRPS29 antibody

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Splice variants that differ in the 5' UTR have been found for this gene; both variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq]. Transcript Variant: This variant (1) is longer than variant 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Kissil JL et al. Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death. J Biol Chem 270:27932-6 (1995). PubMed PMID: 7499268

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DAP3 / MRPS29 (C-term) antibody

TA302460 staining (3µg/ml) of HeLa lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DAP3 / MRPS29 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DAP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802927) Mouse IgG1 OTI1B12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DAP3 / MRPS29 antibody

Predicted band size: 46 kDa . Positive control: 293T and Jurkat cell, human lymphoma tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lane1: 293T cell lysate Lane2: Jurkat cell lysate Lane3: Human Lymphoma tissue lysate Lysates: 30 ug per lane Primary antibody: 1/150 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DAP3 / MRPS29 antibody

Predicted band size: 46 kDa . Positive control: Human lymphoma tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 30 ug Primary antibody: 1/150 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DAP3 / MRPS29 antibody

Predicted band size: 45 kDa . Positive control: Hepg2 and Jurkat cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: Hepg2 cell lysate Lane2: Jurkat cell lysate Lysates: 30 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DAP3 / MRPS29 antibody

Predicted band size: 45 kDa . Positive control: Hepg2 and Hela cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: Hepg2 cell lysate Lane2: Hela cell lysate Lysates: 30 ug per lane Primary antibody: 1/900 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DAP3 / MRPS29 antibody

Western Blot: DAP3 Antibody (42C617.1.2) [TA336396] - Analysis of DAP-3 in A) human brain, B) Jurkat and C) NIH 3T3 lysate using this antibody at 5 ug/ml. Mouse IgG 42C617.1.2 Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DAP3 / MRPS29 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-DAP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802787) Mouse IgG1 OTI1A12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DAP3 / MRPS29 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP3 (RC223182, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAP3. Mouse IgG1 OTI2D4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DAP3 / MRPS29 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP3 (RC223182, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAP3. Mouse IgG1 OTI6E11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DAP3 / MRPS29 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP3 (RC223182, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAP3. Mouse IgG1 OTI10B10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DAP3 / MRPS29 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP3 (RC223182, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAP3. Mouse IgG1 OTI3H6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DAP3 / MRPS29 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP3 (RC223182, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAP3. Mouse IgG1 OTI1B5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DAP3 / MRPS29 antibody

DAP3 / MRPS29 Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

DAP3 / MRPS29 antibody

DAP3 / MRPS29 Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

DAP3 / MRPS29 antibody

DAP3 / MRPS29 Mouse IgG2a 3B7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DAP3 / MRPS29 antibody

Western blot analysis of 10 µg of total cell lysate from Jurkat cells with Dap3 polyclonal antibody ( Cat # PAB0169 ) at 1 µg/ml. Rabbit Hu, Ms, Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

DAP3 / MRPS29 antibody

HEK293 overexpressing DAP3 and probed with DAP3 polyclonal antibody ( Cat # PAB6163 ) ( mock transfection in first lane ), tested by Origene. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

DAP3 / MRPS29 (C-term) antibody

Human U937; WB Suggested Anti-DAP3 Antibody. . Titration: 1.0 ug/ml. . Positive Control: U937 Whole Cell; DAP3 antibody - C-terminal region (ARP61638_P050) in Human U937 cells using Western Blot Rabbit Aff - Purified Hu
50 µg / €350.00
  AVIVA Systems Biology

DAP3 / MRPS29 antibody

NB 100-1032 staining (3µg/ml) of HeLa lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

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