DAPK1 antibody

Principal name

DAPK1 antibody

Alternative names for DAPK1 antibody

Death-associated protein kinase 1, DAP kinase 1, DAPK, DAPK-1, DKFZp781I035

SwissProt ID

P53355 (Human), Q80YE7 (Mouse)

Gene ID

1612 (DAPK1)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Can (Canine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of DAPK1 antibody

Various apoptotic signals use Death Associated Protein Kinase (DAPK) as a downstream effector in different cell types. DAPK is a positive mediator of apoptosis and is widely expressed in many tissues of embryonic and adult origin. DAPK is a Ca2+/calmodulin dependent Ser/Thr kinase that associates with microfilaments. The protein is composed of a multidomain structure. It has a subdomain typical of serine/threonine kinases, a Ca2+/calmodulin regulatory domain, eight ankyrin repeats followed by two P-loop motifs and a typical death domain module. It contains two auto-inhibitory domains one of them Ca2+/calmodulin dependent. In the absence of this latter domain, DAPK is constitutively active. DAPK activity is also regulated by phosphorylation. DAPK was found to be negatively regulated by autophosphorylation at Ser308 which is in the calmodulin regulatory domain. This autophosphorylation, which occurs in cells at the basal state, lowers the affinity of DAPK for calmodulin and thus the kinase is inactive. Under some apoptotic conditions DAPK undergoes dephosphorylation. As a consequence, it binds to calmodulin with higher affinity, becomes activated, phosphorylates its downstream substrate proteins, and mediates apoptosis. Monoclonal antibodies specific for Phospho DAP-Kinase (pSer308) are important tools for studying the mechanism of DAPK activation in apoptosis.

General readings

General / background references:Cohen O et al. DAP-kinase participates in TNF-alpha- and Fas-induced apoptosis and its function requires the death domain. J Cell Biol 146:141-8 (1999). PubMed PMID: 10402466
Cohen O et al. DAP-kinase is a Ca2+/calmodulin-dependent, cytoskeletal-associated protein kinase, with cell death-inducing functions that depend on its catalytic activity. EMBO J 16:998-1008 (1997). PubMed PMID: 9118961
Deiss LP et al. Cathepsin D protease mediates programmed cell death induced by interferon-gamma, Fas/APO-1 and TNF-alpha. EMBO J 15:3861-70 (1996). PubMed PMID: 8670891

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DAPK1 (C-term) antibody

The DAPK1 polyclonal antibody (Cat. #TA324907) is used in Western blot to detect DAPK1 in mouse liver tissue lysate. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

DAPK1 antibody

Western blot analysis of extracts of various cell lines, using DAPK1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

DAPK1 antibody

WB Suggested Anti-DAPK1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Can, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

DAPK1 antibody

ELISA: DAP Kinase 1 Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

DAPK1 antibody

DAPK1 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

DAPK1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between MAPK3 and DAPK1. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-DAPK1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG1 2E7 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

DAPK1 antibody

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with DAPK1 polyclonal antibody ( Cat # PAB2049 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

DAPK1 antibody

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using DAPK1 (Ab-308) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P
50 µg / €205.00
  Assay Biotech

DAPK1 antibody

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using DAPK1 (Ab-308) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P
0.1 mg / €310.00
  Assay Biotech

DAPK1 antibody

Human THP-1; WB Suggested Anti-DAPK1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: THP-1 cell lysate; DAPK1 antibody - N-terminal region (ARP58219_P050) in Human THP-1 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

DAPK1 antibody

ELISA: DAP Kinase 1 Antibody [28480002] Rabbit Hu E
50 µg / €470.00
  Novus Biologicals Inc.

DAPK1 antibody

DAPK1 Mouse IgG1 DKPS308 Aff - Purified Hu E, IP, WB
50 µl / €340.00
  Novus Biologicals Inc.

DAPK1 antibody

DAPK1 Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DAPK1

DAPK1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

DAPK1

DAPK1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

DAPK1

DAPK1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

DAPK1

DAPK1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

DAPK1

DAPK1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

DAPK1

DAPK1 Human
0.1 mg / €1,260.00
  Abnova Taiwan Corp.

DAPK1

DAPK1 Human
5 µg / €520.00
  Abnova Taiwan Corp.

20 Item(s)

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