DARPP32 antibody

Principal name

DARPP32 antibody

Alternative names for DARPP32 antibody

PPP1R1B, DARPP-32, FLJ20940, Protein phosphatase 1 regulatory subunit 1B, Dopamine- and cAMP-regulated neuronal phosphoprotein

SwissProt ID

P07516 (Bovin), Q29277 (Pig), Q60829 (Mouse), Q6J4I0 (Rat), Q9UD71 (Human)

Gene ID

84152 (PPP1R1B), 360616 (Ppp1r1b)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Mky (Monkey), Xen (Xenopus), Ze (Zebrafish), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of DARPP32 antibody

Dopaminergic signaling pathways, which are essential for multiple brain functions, are abnormal in several neurological disorders, such as schizophrenia, Parkinson’s disease and drug abuse. DARPP-32 (for dopamine and adenosine 3’,5’-monophosphate-regulated phosphoprotein of 32 kDa) is abundant in neurons that receive dopaminergic input. Activation of PKA and the consequent phosphorylation of DARPP-32 on threonine occurs in response to dopamine acting upon D1-like receptors. Dopamine interaction with D2-like receptors results in the inhibition of PKA activation, the activation of protein phosphatase 2B and the consequent dephosphorylation of DARPP-32. Neurotransmitters other than dopamine may also be able to stimulate the phosphorylation or dephosphorylation of DARPP-32. Phosphorylated DARPP-32 is a potent inhibitor of PP-1.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DARPP32 (N-term) antibody

Western blot of a rat hippocampal lysate showing specific immunolabeling of the ~32k DARPP protein. Rabbit IgG Aff - Purified Bov, Can, Chk, Hu, Ms, Rt, Xen, Ze C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DARPP32 pThr34 antibody

Figure 1. Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr34 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr34 DARPP-32. The immunolabeling is completely eliminated by treatment with lambda-Ptase. Rabbit IgG Aff - Purified Bov, Can, Chk, Hu, Ms, non-Human Primates, Rt WB
0.1 ml / €380.00
  Acris Antibodies GmbH

DARPP32 pThr75 antibody

Figure 1. Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr75 DARPP-32. The immunolabeling is completely eliminated by treatment with lambda-Ptase. Rabbit IgG Aff - Purified Bov, Can, Chk, Hu, Ms, non-Human Primates, Rt WB
0.1 ml / €380.00
  Acris Antibodies GmbH

DARPP32 antibody

Western blot analysis of extracts from 293 cells treated with EGF (200ng/ml, 30min), using DARPP-32 (Ab-75) antibody (#TA312428, Line 1 and 2).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DARPP32 antibody

Western blot analysis of DARPP-32 expression in EGF treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DARPP32 antibody

Western blot analysis of DARPP-32 expression in forskolin treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DARPP32 antibody

DARPP32 Rabbit IgG Purified Bov, Can, Chk, Hu, Ms, Rt, Xen, Ze ICC/IF, WB
0.1 ml / €510.00
  Acris Antibodies GmbH

DARPP32 pThr34 antibody

DARPP32 Rabbit IgG Purified Bov, Can, Chk, Hu, Mky, Ms, Rt WB
0.1 ml / €510.00
  Acris Antibodies GmbH

DARPP32 pThr75 antibody

DARPP32 Rabbit IgG Purified Bov, Can, Chk, Hu, Mky, Ms, Rt WB
0.1 ml / €510.00
  Acris Antibodies GmbH

DARPP32 antibody

Immunohistochemistry (IHC) analyzes of DARPP-32 antibody (Cat.-No.: AP06383PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

DARPP32 pThr75 antibody

Immunofluorescence staining of methanol-fixed HeLa cells using DARPP-32 (Phospho-Thr75) Antibody Rabbit Aff - Purified Hu, Ms, Rt ICC/IF
0.1 ml / €320.00
  Acris Antibodies GmbH

DARPP32 pThr75 antibody

Immunofluorescence staining of methanol-fixed HeLa cells using DARPP-32 (Phospho-Thr75) Antibody Rabbit Aff - Purified Hu, Ms, Rt ICC/IF
50 µl / €240.00
  Acris Antibodies GmbH

DARPP32 pSer137 antibody

Western blot of rat caudate lysate showing specific immunolabeling of the ~32k
DARPP-32 phosphorylated at Thr137 (Control). Phosphospecificity is shown in the second lane where immunolabeling is blocked by preadsorption of the phospho-peptide used as antigen (peptide) but not by the corresponding dephosphopeptide (not shown). Rabbit IgG Aff - Purified Bov, Can, Chk, Hu, Ms, non-Human Primates, Rt WB
0.1 ml / €380.00
  Acris Antibodies GmbH

DARPP32 antibody

Western Blot analysis of PPP1R1B expression in transfected 293T cell line (H00084152-T01) by PPP1R1B MaxPab polyclonal antibody.

Lane 1: PPP1R1B transfected lysate(22.44 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

DARPP32 antibody

Western Blot analysis of PPP1R1B expression in transfected 293T cell line (H00084152-T01) by PPP1R1B MaxPab polyclonal antibody.

Lane 1: PPP1R1B transfected lysate(22.44 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

DARPP32 antibody

DARPP32 Mouse IgG2b 3G11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DARPP32 antibody

DARPP32 Mouse IgG1 1A3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DARPP32 antibody

Immunochemical staining of cultured mouse caudate neurons with Ppp1r1b polyclonal antibody ( Cat # PAB9626 ). Cells and photo courtesy of QBM Cell Science. Rabbit Bov, Chk, Hu, Ms, Rt ICC/IF, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

DARPP32 pThr34 antibody

Western blot of rat caudate lysate showing specific immunolabeling of the ~32k Ppp1r1b phosphorylated at Thr34 (Control, lane 1). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase, lane 2). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Ppp1r1b (phospho T34) polyclonal antibody ( Cat # PAB9669 ). The immunolabeling is completely eliminated by treatment with l-Ptase. Rabbit Bov, Chk, Hu, Ms, Rt WB
0.1 ml / €510.00
  Abnova Taiwan Corp.

DARPP32 pThr75 antibody

Western blot of rat caudate lysate showing specific immunolabeling of the ~32k Ppp1r1b phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Ppp1r1b (phospho T75) polyclonal antibody ( Cat # PAB9670 ). The immunolabeling is completely eliminated by treatment with l-Ptase. Rabbit Bov, Chk, Hu, Ms, Rt WB
0.1 ml / €510.00
  Abnova Taiwan Corp.

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