DARPP32 antibody

Principal name

DARPP32 antibody

Alternative names for DARPP32 antibody

PPP1R1B, DARPP-32, FLJ20940, Protein phosphatase 1 regulatory subunit 1B, Dopamine- and cAMP-regulated neuronal phosphoprotein

SwissProt ID

P07516 (Bovin), Q29277 (Pig), Q60829 (Mouse), Q6J4I0 (Rat), Q9UD71 (Human)

Gene ID

84152 (PPP1R1B), 360616 (Ppp1r1b), 19049

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Mky (Monkey), Xen (Xenopus), Ze (Zebrafish), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Frozen Sections (C)

Background of DARPP32 antibody

Kit Component:
- KN313743G1, Ppp1r1b gRNA vector 1 in pCas-Guide vector
- KN313743G2, Ppp1r1b gRNA vector 2 in pCas-Guide vector
- KN313743D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DARPP32 (N-term) antibody

Western blot of a rat hippocampal lysate showing specific immunolabeling of the ~32k DARPP protein. Rabbit IgG Aff - Purified Bov, Can, Chk, Hu, Ms, Rt, Xen, Ze C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DARPP32 pThr34 antibody

Figure 1. Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr34 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr34 DARPP-32. The immunolabeling is completely eliminated by treatment with lambda-Ptase. Rabbit IgG Aff - Purified Bov, Can, Chk, Hu, Ms, non-Human Primates, Rt WB
0.1 ml / €380.00
  Acris Antibodies GmbH

DARPP32 pThr75 antibody

Figure 1. Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr75 DARPP-32. The immunolabeling is completely eliminated by treatment with lambda-Ptase. Rabbit IgG Aff - Purified Bov, Can, Chk, Hu, Ms, non-Human Primates, Rt WB
0.1 ml / €380.00
  Acris Antibodies GmbH

DARPP32 antibody

Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr34 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr34 DARPP-32. The immunolabeling is completely eliminated by treatment with λ-Ptase. Rabbit IgG Purified Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DARPP32 antibody

Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr75 DARPP-32. The immunolabeling is completely eliminated by treatment with λ-Ptase. Rabbit IgG Purified Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DARPP32 antibody

Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr137 (Control). Phosphospecificity is shown in the second lane where immunolabeling is blocked by preadsorption of the phospho-peptide used as antigen (peptide) but not by the corresponding dephosphopeptide (not shown). Rabbit IgG Purified Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DARPP32 antibody

Western blot analysis of extracts from COS7 cells treated with Forskolin (40nM, 30mins), using DARPP-32 (phospho-Thr75) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DARPP32 antibody

Western blot analysis of extracts from 3T3 cells, treated with PMA (125ng/ml, 30mins), using DARPP-32 (Phospho-Thr34) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DARPP32 antibody

Western blot analysis of extracts from 293 cells treated with EGF (200ng/ml, 30min), using DARPP-32 (Ab-75) antibody (#TA312428, Line 1 and 2).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DARPP32 antibody

Western blot analysis of DARPP-32 expression in EGF treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DARPP32 antibody

Western blot analysis of DARPP-32 expression in forskolin treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DARPP32 antibody

Western blot analysis of DARPP-32 phosphorylation expression in PMA treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DARPP32 antibody

Western blot analysis of DARPP-32 phosphorylation expression in Forskolin treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DARPP32 antibody

DARPP32 Rabbit IgG Purified Bov, Can, Chk, Hu, Ms, Rt, Xen, Ze ICC/IF, WB
0.1 ml / €510.00
  Acris Antibodies GmbH

DARPP32 pThr34 antibody

DARPP32 Rabbit IgG Purified Bov, Can, Chk, Hu, Mky, Ms, Rt WB
0.1 ml / €510.00
  Acris Antibodies GmbH

DARPP32 pThr75 antibody

DARPP32 Rabbit IgG Purified Bov, Can, Chk, Hu, Mky, Ms, Rt WB
0.1 ml / €510.00
  Acris Antibodies GmbH

DARPP32 antibody

Immunohistochemistry (IHC) analyzes of DARPP-32 antibody (Cat.-No.: AP06383PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

DARPP32 pThr75 antibody

Immunofluorescence staining of methanol-fixed HeLa cells using DARPP-32 (Phospho-Thr75) Antibody Rabbit Aff - Purified Hu, Ms, Rt ICC/IF
0.1 ml / €320.00
  Acris Antibodies GmbH

DARPP32 pThr75 antibody

Immunofluorescence staining of methanol-fixed HeLa cells using DARPP-32 (Phospho-Thr75) Antibody Rabbit Aff - Purified Hu, Ms, Rt ICC/IF
50 µl / €240.00
  Acris Antibodies GmbH

DARPP32 pSer137 antibody

Western blot of rat caudate lysate showing specific immunolabeling of the ~32k
DARPP-32 phosphorylated at Thr137 (Control). Phosphospecificity is shown in the second lane where immunolabeling is blocked by preadsorption of the phospho-peptide used as antigen (peptide) but not by the corresponding dephosphopeptide (not shown). Rabbit IgG Aff - Purified Bov, Can, Chk, Hu, Ms, non-Human Primates, Rt WB
0.1 ml / €380.00
  Acris Antibodies GmbH

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