DAXX antibody

Principal name

DAXX antibody

Alternative names for DAXX antibody

BING2, DAP6, EAP1, Death domain-associated protein 6, Fas death domain-associated protein, ETS1-associated protein 1

SwissProt ID

O35613 (Mouse), Q5TJE1 (Canfa), Q8VIB2 (Rat), Q9UER7 (Human)

Gene ID

1616 (DAXX), 13163 (Daxx)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Bov (Bovine), Can (Canine), Eq (Equine)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), Frozen Sections (C), ELISA (detection) (E(detection))

Background of DAXX antibody

DAXX(death-domain associated protein) also known as DAP6(Death-associated protein 6) or BING2, was first discovered through its cytoplasmic interaction with the classical death receptor Fas. Human DAXX encodes a 740-amino acid polypeptide containing a nuclear localization signal. Functional analyses by Yang et al. (1997) demonstrated that Daxx binds to the Fas death domain and enhances Fas-mediated apoptosis. The authors suggested that DAXX and FADD define 2 distinct apoptotic pathways downstream of Fas. The DAXX gene is mapped to human chromosome 6p21.3 by somatic cell hybrid panels and fluorescence in situ hybridization, a region containing the HLA and putative autoimmune disease genes. MSP58 overexpression relieved DAXX-mediated transcriptional repression. Immunoprecipitation and Western blot analysis with DAXX mutants showed that the N terminus of DAXX interacts with the C terminus of DMAP. Transient expression of DAXX or DMAP1 caused repression of glucocorticoid receptor-mediated transcription.

General readings

1. Meinecke, I., et al., PNAS 104, 5073-5078 (2007)
2. Luciani, J. J., et al., J. Cell Sci. 119, 2518-2531 (2006)
3. Vassiliki, S., et al., J. Biol. Chem. 277, 19783-19791 (2002)
4. Cermak, L., et al., J. Biol. Chem. 277, 7955-7961 (2002)
5. Torii, S., et al., EMBO 18, 6037-6049 (1999)
Clone DAXX-01 is used in references 1 and 2.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DAXX antibody

Confocal immunofluorescence analysis of PANC-1 cells using DAXX mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Mouse IgG1 7A11 Aff - Purified Hu E, ICC/IF, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

DAXX (C-term) antibody

Western blot analysis of Daxx in 293 total cell lysate with Dax antibody at 1 mg/mL. Rabbit IgG Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DAXX (261-274) antibody

Western blot using affinity purified anti-DAXX antibody shows detection of DAXX in RWPE-1 cell extracts. The membrane was probed with the primary antibody diluted to 1:200, and further probed with peroxidase conjugated anti-Rabbit IgG at a 1:25,000 dilution. Personal Communication, Jie LIU, CCR-NCI, Bethesda, MD. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DAXX antibody

Host: Rabbit; Target Name: DAXX; Sample Tissue: HepG2 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Can, Eq, Hu, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

DAXX antibody

DAXX Mouse IgG1 DAXX-03 Aff - Purified Hu ICC/IF, IP, WB
0.1 mg / €230.00
  Acris Antibodies GmbH

DAXX (C-term) antibody

Immunocytochemical staining of HeLa cells with Rabbit anti DAXX (AP05800PU-N) Rabbit IgG Purified Hu, Mky, Ms ICC/IF, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

DAXX antibody

Immunohistochemistry (IHC) analyzes of Daxx antibody (Cat.-No.: AP06384PU-N) in paraffin-embedded human ovary tissue. Rabbit Aff - Purified Hu P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

DAXX antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

DAXX pSer213 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DAXX antibody

Western blot (WB) analysis of Daxx antibody (Cat.-No.: AP20484PU-N) in extracts from 293 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

DAXX Control Peptide

DAXX
50 µg / €160.00
  Acris Antibodies GmbH

DAXX (C-term) antibody

Western blot anaylsis of Daxx expression in Raji cells (Lane 1), Jurkat cells (Lane 2), WR19L cells (Lane 3) and PC12 cells (Lane 4) using SM1821A Daxx antibody Mouse IgG1 DAXX-01 Azide Free Hu ICC/IF, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

DAXX (C-term) antibody

Figure 2: Immunofluorescence staining of Daxx in transfected HeLa Human cervix carcinoma cell line. Myc Daxx (green) was stained with anti-Human Daxx (DAXX-01), nuclei were stained with DAPI (blue).
HeLa cells were co-transfected with pCDNA3-MycDaxx and pCDNA3-ASK1HA, which led to translocation of Daxx from nucleus to cytoplasm. Mouse IgG1 DAXX-01 Purified Hu, Ms ICC/IF, WB
0.1 mg / €230.00
  Acris Antibodies GmbH

DAXX (561- 661) antibody

DAXX Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

DAXX antibody

Western Blot analysis of DAXX expression in transfected 293T cell line (H00001616-T02) by DAXX MaxPab polyclonal antibody.

Lane 1: DAXX transfected lysate(81.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

DAXX antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between TGFB1 and DAXX HeLa cells were stained with anti-TGFB1 rabbit purified polyclonal 1:1200 and anti-DAXX mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2b 4C2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DAXX antibody

Immunofluorescence staining of DAXX in transfected HeLa human cervix carcinoma cell line. DAXX ( green ) was stained with DAXX monoclonal antibody, clone DAXX - 01 ( Cat # MAB0937 ) , nuclei were stained with DAPI ( blue ) . A : Nuclear localization of DAXX in HeLa cells transfected with pCDNA3 - MycDaxx. B : HeLa cells were co - transfected with pCDNA3 - MycDaxx and pCDNA3 - ASK1HA, which led to translocation of DAXX from nucleus to cytoplasm. Mouse IgG1 DAXX-01 Hu ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

DAXX (272-291) antibody

Immunohistochemical analysis of DAXX in paraffin-embedded formalin-fixed human mammary gland ( female breast ) using DAXX polyclonal antibody ( Cat # PAB0254 ) at 1 : 2000. A, normal breast tissue. B, ductal carcinoma in situ ( DCIS ) . C. invasive neoplasia. DAXX expressed increased sucessively with tumor progression. Hematoxylin-eosin counterstain. Rabbit Bov, Hu, Mky, Rt C, IP, P
50 µl / €390.00
  Abnova Taiwan Corp.

DAXX pSer213 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with DAXX (phospho S213) polyclonal antibody ( Cat # PAB0448 ) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu P, WB
0.4 ml / €400.00
  Abnova Taiwan Corp.

DAXX antibody

Western blot using DAXX polyclonal antibody ( Cat # PAB10002 ) shows detection of DAXX in RWPE-1 cell extracts.
The membrane was probed with the primary antibody diluted to 1 : 200, and further probed with peroxidase conjugated anti-Rabbit IgG at a 1 : 25,000 dilution.
Personal Communication, Jie LIU, CCR-NCI, Bethesda, MD. Hu, Ms, Rt
0.1 mg / €560.00
  Abnova Taiwan Corp.

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