DAXX antibody

Principal name

DAXX antibody

Alternative names for DAXX antibody

BING2, DAP6, EAP1, Death domain-associated protein 6, Fas death domain-associated protein, ETS1-associated protein 1

SwissProt ID

O35613 (Mouse), Q5TJE1 (Canfa), Q8VIB2 (Rat), Q9UER7 (Human)

Gene ID

1616 (DAXX)

Available reactivities

Hu (Human), Ms (Mouse), Mky (Monkey), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C), Dot blot (Dot)

Background of DAXX antibody

Acts as an adapter protein in a MDM2-DAXX-USP7 complex by regulating the RING-finger E3 ligase MDM2 ubiquitination activity. Under non-stress condition, in association with the deubiquitinating USP7, prevents MDM2 self-ubiquitination and enhances the intrinsic E3 ligase activity of MDM2 towards TP53, thereby promoting TP53 ubiquitination and subsequent proteasomal degradation. Upon DNA damage, its association with MDM2 and USP7 is disrupted, resulting in increased MDM2 autoubiquitination and consequently, MDM2 degradation, which leads to TP53 stabilization. Proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. Down-regulates basal and activated transcription. Seems to act as a transcriptional corepressor and inhibits PAX3 and ETS1 through direct protein-protein interaction. Modulates PAX5 activity. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively.

General readings

1. Meinecke, I., et al., PNAS 104, 5073-5078 (2007)
2. Luciani, J. J., et al., J. Cell Sci. 119, 2518-2531 (2006)
3. Vassiliki, S., et al., J. Biol. Chem. 277, 19783-19791 (2002)
4. Cermak, L., et al., J. Biol. Chem. 277, 7955-7961 (2002)
5. Torii, S., et al., EMBO 18, 6037-6049 (1999)
Clone DAXX-01 is used in references 1 and 2.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DAXX antibody

Confocal immunofluorescence analysis of PANC-1 cells using DAXX mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Mouse IgG1 7A11 Aff - Purified Hu E, ICC/IF, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

DAXX antibody

Immunofluorescence: DAXX Antibody (7A11) [NBP1-47453] - Confocal immunofluorescence analysis of PANC-1 cells using anti-DAXX mAb (green). Blue: DRAQ5 fluorescent DNA dye. Mouse IgG1 7A11 Aff - Purified Hu E, ICC/IF, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

DAXX (C-term) antibody

Western blot analysis of Daxx in 293 total cell lysate with Dax antibody at 1 mg/mL. Rabbit IgG Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DAXX antibody

Western blot analysis of extracts from COS7 cells, treated with Serum (10%, 30mins), using Daxx (Ab-668) Antibody (#TA311875).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DAXX antibody

Western blot analysis of extracts from 293 cells and A549 cells, using DAXX antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DAXX (261-274) antibody

Western blot using affinity purified anti-DAXX antibody shows detection of DAXX in RWPE-1 cell extracts. The membrane was probed with the primary antibody diluted to 1:200, and further probed with peroxidase conjugated anti-Rabbit IgG at a 1:25,000 dilution. Personal Communication, Jie LIU, CCR-NCI, Bethesda, MD. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DAXX antibody

Host: Rabbit; Target Name: DAXX; Sample Tissue: HepG2 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Can, Eq, Hu, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

DAXX antibody

DAXX Mouse IgG1 DAXX-03 Aff - Purified Hu ICC/IF, IP, WB
0.1 mg / €230.00
  Acris Antibodies GmbH

DAXX (C-term) antibody

Immunocytochemical staining of HeLa cells with Rabbit anti DAXX (AP05800PU-N) Rabbit IgG Purified Hu, Mky, Ms ICC/IF, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

DAXX antibody

Immunohistochemistry (IHC) analyzes of Daxx antibody (Cat.-No.: AP06384PU-N) in paraffin-embedded human ovary tissue. Rabbit Aff - Purified Hu P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

DAXX antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

DAXX pSer213 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DAXX antibody

Western blot (WB) analysis of Daxx antibody (Cat.-No.: AP20484PU-N) in extracts from 293 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

DAXX Control Peptide

DAXX
50 µg / €160.00
  Acris Antibodies GmbH

DAXX (C-term) antibody

Western blot anaylsis of Daxx expression in Raji cells (Lane 1), Jurkat cells (Lane 2), WR19L cells (Lane 3) and PC12 cells (Lane 4) using SM1821A Daxx antibody Mouse IgG1 DAXX-01 Azide Free Hu ICC/IF, WB
0.1 mg / €310.00
  Acris Antibodies GmbH

DAXX (C-term) antibody

Figure 2: Immunofluorescence staining of Daxx in transfected HeLa Human cervix carcinoma cell line. Myc Daxx (green) was stained with anti-Human Daxx (DAXX-01), nuclei were stained with DAPI (blue).
HeLa cells were co-transfected with pCDNA3-MycDaxx and pCDNA3-ASK1HA, which led to translocation of Daxx from nucleus to cytoplasm. Mouse IgG1 DAXX-01 Purified Hu, Ms ICC/IF, WB
0.1 mg / €230.00
  Acris Antibodies GmbH

DAXX (561- 661) antibody

DAXX Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

DAXX antibody

Western Blot analysis of DAXX expression in transfected 293T cell line (H00001616-T02) by DAXX MaxPab polyclonal antibody.

Lane 1: DAXX transfected lysate(81.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

DAXX antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between TGFB1 and DAXX HeLa cells were stained with anti-TGFB1 rabbit purified polyclonal 1:1200 and anti-DAXX mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2b 4C2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DAXX antibody

Immunofluorescence staining of DAXX in transfected HeLa human cervix carcinoma cell line. DAXX ( green ) was stained with DAXX monoclonal antibody, clone DAXX - 01 ( Cat # MAB0937 ) , nuclei were stained with DAPI ( blue ) . A : Nuclear localization of DAXX in HeLa cells transfected with pCDNA3 - MycDaxx. B : HeLa cells were co - transfected with pCDNA3 - MycDaxx and pCDNA3 - ASK1HA, which led to translocation of DAXX from nucleus to cytoplasm. Mouse IgG1 DAXX-01 Hu ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

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