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DBF4 / ASK antibody

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Principal name

DBF4 / ASK antibody

Alternative names for DBF4 / ASK antibody

DBF4A, ZDBF1, Chiffon homolog A, Protein DBF4 homolog A, Activator of S phase kinase, DBF4-type zinc finger-containing protein 1

SwissProt ID

Q9QZ41 (Mouse), Q9UBU7 (Human)

Gene ID

10926 (DBF4), 27214 (Dbf4)

Ncbi ID

9606, NP_006707, NP_006707.1, AAH36045, NM_006716, NP_001177646, NP_001177646.1, NM_001190717, NM_013726

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Por (Porcine), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of DBF4 / ASK antibody

The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7-Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase alpha-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4, and the kinase activity of the complex is regulated throughout the cell cycle mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosome separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire.

H00010926-M01 - DBF4 / ASK
Immunoperoxidase of monoclonal antibody to DBF4 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml]
H00010926-M01 - DBF4 / ASK
Detection limit for recombinant GST tagged DBF4 is approximately 0.1ng/ml as a capture antibody.
H00010926-A01 - DBF4 / ASK
DBF4 polyclonal antibody (A01), Lot # 060614JCS1 Western Blot analysis of DBF4 expression in Jurkat ( Cat # L017V1 ).
H00010926-D01P - DBF4 / ASK
Western Blot analysis of DBF4 expression in transfected 293T cell line (H00010926-T03) by DBF4 MaxPab polyclonal antibody.Lane 1: DBF4 transfected lysate(76.90 KDa).Lane 2: Non-transfected lysate.
AP20463PU-N - DBF4 / ASK
Western blot (WB) analysis of Dbf4 antibody (Cat.-No.: AP20463PU-N) in extracts from NIH-3T3 treated with H2O2 100uM 30'.
C11821-1 - DBF4 / ASK
Western blot analysis of extracts from NIH-3T3 cells, treated with H2O2 100uM 30', COS7 treated with PMA 125ng/ml 30',using DBF4 Antibody. The lane on the right is treated with the synthesized peptide.
CPA2519-100ul - DBF4 / ASK
Immunohistochemical analysis of DBF4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA2519-100ul - DBF4 / ASK
Immunofluorescent analysis of DBF4 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CPA2519-100ul - DBF4 / ASK
Western blot analysis of DBF4 expression in Jurkat (A); HepG2 (B); SW480 (C) whole cell lysates.
TA311409 - DBF4 / ASK
(1ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA315124 - DBF4 / ASK
Western blot analysis of extracts from NIH-3T3 cells treated with H2O2 (100uM, 30mins) and COS-7 cells treated with PMA (125mg/ml, 30mins), using DBF4 antibody.The lane on the right is treated with the synthesized peptide.

20 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DBF4 / ASK antibody
  Goat Anti-Dbf4 (mouse) Antibody  
(1ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Ms, Rt E, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

DBF4 / ASK antibody
  Rabbit polyclonal anti-DBF4 antibody  
Western blot analysis of extracts from NIH-3T3 cells treated with H2O2 (100uM, 30mins) and COS-7 cells treated with PMA (125mg/ml, 30mins), using DBF4 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

DBF4 / ASK antibody
Western blot (WB) analysis of Dbf4 antibody (Cat.-No.: AP20463PU-N) in extracts from NIH-3T3 treated with H2O2 100uM 30'. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

DBF4 / ASK antibody
Western blot analysis of extracts from NIH-3T3 cells, treated with H2O2 100uM 30', COS7 treated with PMA 125ng/ml 30',using DBF4 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €230.00
  Assay Biotech

DBF4 / ASK antibody
Western blot analysis of extracts from NIH-3T3 cells, treated with H2O2 100uM 30', COS7 treated with PMA 125ng/ml 30',using DBF4 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

DBF4 - human gene knockout kit via CRISPR
DBF4 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

DBF4 - human gene knockout kit via CRISPR
DBF4 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

DBF4 - human gene knockout kit via CRISPR
DBF4 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

DBF4 - human gene knockout kit via CRISPR
DBF4 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dbf4 - mouse gene knockout kit via CRISPR
Dbf4 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dbf4 - mouse gene knockout kit via CRISPR
Dbf4 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dbf4 - mouse gene knockout kit via CRISPR
Dbf4 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dbf4 - mouse gene knockout kit via CRISPR
Dbf4 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

DBF4 overexpression lysate
DBF4 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, DBF4 (mGFP-tagged) - Human DBF4 homolog (S. cerevisiae) (DBF4), 200 uL, >10^7 TU/mL
Lenti ORF particles, DBF4 (mGFP-tagged) - Human DBF4 homolog (S. cerevisiae) (DBF4), 200 uL, >10^7 TU/mL  
200 µl / €1,082.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, DBF4 (Myc-DDK tagged) - Human DBF4 homolog (S. cerevisiae) (DBF4) , 200 uL, >10^7 TU/mL
Lenti ORF particles, DBF4 (Myc-DDK tagged) - Human DBF4 homolog (S. cerevisiae) (DBF4) , 200 uL, >10^7 TU/mL  
200 µl / €969.00
  OriGene Technologies, Inc.
 

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Dbf4 (GFP-tagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4), transcript variant 1, 200 uL, >10^7 TU/mL
Lenti ORF particles, Dbf4 (GFP-tagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,381.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Dbf4 (GFP-tagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4), transcript variant 2, 200 uL, >10^7 TU/mL
Lenti ORF particles, Dbf4 (GFP-tagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,381.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Dbf4 (Myc-DDK-tagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4), transcript variant 1, 200 uL, >10^7 TU/mL
Lenti ORF particles, Dbf4 (Myc-DDK-tagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,236.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Dbf4 (Myc-DDK-tagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4), transcript variant 2, 200 uL, >10^7 TU/mL
Lenti ORF particles, Dbf4 (Myc-DDK-tagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,236.00
  OriGene Technologies, Inc.
 

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Available Filters

Product Type
  1. CRISPR (8)
  2. Human Lenti ORF Particle (2)
  3. Lysate (1)
  4. Mouse Lenti ORF Particle (4)
  5. Primary Antibody (5)
Clonality
  1. Polyclonal (5)
Target
  1. DBF4 / ASK (5)
Reactivity
  1. Mouse (Ms) (5)
  2. Human (Hu) (4)
  3. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Enzyme Immunoassay (E) (4)
  3. Paraffin Sections (P) (1)
Presentation
  1. Aff - Purified (4)
  2. Purified (1)
Host
  1. Rabbit (4)
  2. Goat (1)
Images
  1. 1 image (5)
Images: Applications
  1. Western blot / Immunoblot (WB) (5)
Species (CRISPR)
  1. Human (4)
  2. Mouse (4)
Donor Vector
  1. GFP-Puro (2)
  2. Luciferase-Puro (2)
  3. RFP-BSD (2)
  4. mBFP-Neo (2)
Manufacturer
  1. Acris - OriGene (18)
  2. Assay Biotech (2)
Service
  • OriGene EU
  • Acris Antibodies GmbH
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  • D-32052 Herford
  • Germany
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  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
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