DBF4 / ASK antibody

Principal name

DBF4 / ASK antibody

Alternative names for DBF4 / ASK antibody

DBF4A, ZDBF1, Chiffon homolog A, Protein DBF4 homolog A, Activator of S phase kinase, DBF4-type zinc finger-containing protein 1

SwissProt ID

Q9QZ41 (Mouse), Q9UBU7 (Human)

Gene ID

10926 (DBF4), 216 (ALDH1A1), 1092663 (P65413), 27214 (Dbf4)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of DBF4 / ASK antibody

The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7-Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase alpha-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4, and the kinase activity of the complex is regulated throughout the cell cycle mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosome separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DBF4 / ASK antibody

(1ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DBF4 / ASK antibody

Western blot analysis of extracts from NIH-3T3 cells treated with H2O2 (100uM, 30mins) and COS-7 cells treated with PMA (125mg/ml, 30mins), using DBF4 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DBF4 / ASK antibody

Western blot (WB) analysis of Dbf4 antibody (Cat.-No.: AP20463PU-N) in extracts from NIH-3T3 treated with H2O2 100uM 30'. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

DBF4 / ASK antibody

DBF4 polyclonal antibody (A01), Lot # 060614JCS1 Western Blot analysis of DBF4 expression in Jurkat ( Cat # L017V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

DBF4 / ASK antibody

Western Blot analysis of DBF4 expression in transfected 293T cell line (H00010926-T03) by DBF4 MaxPab polyclonal antibody.

Lane 1: DBF4 transfected lysate(76.90 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

DBF4 / ASK antibody

Immunoperoxidase of monoclonal antibody to DBF4 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml] Mouse IgG1 6G9 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DBF4 / ASK antibody

Western blot analysis of extracts from NIH-3T3 cells, treated with H2O2 100uM 30', COS7 treated with PMA 125ng/ml 30',using DBF4 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

DBF4 / ASK antibody

Western blot analysis of extracts from NIH-3T3 cells, treated with H2O2 100uM 30', COS7 treated with PMA 125ng/ml 30',using DBF4 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

DBF4 / ASK antibody

Immunohistochemical analysis of DBF4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Por ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

DBF4 / ASK antibody

Immunohistochemical analysis of DBF4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Por ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

DBF4 / ASK antibody

Immunohistochemical analysis of DBF4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Por ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

DBF4 / ASK antibody

Western Blot: DBF4 Antibody [NB100-88115] - Detection of Human DBF4 by Western Blot of  Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP,  20% of IP loaded) from HeLa cells. Antibodies: Affinity purified  rabbit anti-DBF4 antibody NB100-88115 used for IP at 3 mcg/mg  lysate. DBF4 was also immunoprecipitated by rabbit anti-DBF4  antibody NB100-88114, which recognizes an upstream epitope. Detection: Chemiluminescence with  an exposure time of 30 seconds. Rabbit IgG Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

DBF4 / ASK antibody

Western Blot: Dbf4 Antibody [NBP1-19618] - extracts from NIH-3T3 treated with H2O2 100uM 30'. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

DBF4 / ASK antibody

DBF4 / ASK Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

DBF4 / ASK antibody

DBF4 / ASK Rabbit IgG Aff - Purified Hu, Ms E, WB
50 µg / €290.00
  Novus Biologicals Inc.

DBF4 / ASK antibody

Western Blot: DBF4 Antibody [NBP1-78282] - (1ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Ms, Rt E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DBF4 / ASK

DBF4 / ASK Human
2 µg / €300.00
  Abnova Taiwan Corp.

DBF4 / ASK

DBF4 / ASK Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

DBF4 / ASK

DBF4 / ASK Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

DBF4 / ASK

DBF4 / ASK Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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