DBF4 / ASK antibody
Principal name
DBF4 / ASK antibody
Alternative names for DBF4 / ASK antibody
DBF4A, ZDBF1, Chiffon homolog A, Protein DBF4 homolog A, Activator of S phase kinase, DBF4-type zinc finger-containing protein 1
Ncbi ID
9606, NP_006707, NP_006707.1, NP_001177646, AAH36045, NM_006716, NP_001177646.1
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of DBF4 / ASK antibody
The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7-Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase alpha-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4, and the kinase activity of the complex is regulated throughout the cell cycle mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosome separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire.
Immunoperoxidase of monoclonal antibody to DBF4 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml]
Detection limit for recombinant GST tagged DBF4 is approximately 0.1ng/ml as a capture antibody.
DBF4 polyclonal antibody (A01), Lot # 060614JCS1 Western Blot analysis of DBF4 expression in Jurkat ( Cat # L017V1 ).
Western Blot: DBF4 Antibody [NB100-88115] - Detection of Human DBF4 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DBF4 antibody NB100-88115 used for IP at 3 mcg/mg lysate. DBF4 was also immunoprecipitated by rabbit anti-DBF4 antibody NB100-88114, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.
Formalin-fixed and paraffin-embedded human testis tissue reacted with ASK antibody (N-term) (Cat#AP14024PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-ASK Pab (Cat#AP14024PU-N) in HepG2 cell line lysates (35ug/lane). ASK(arrow) was detected using the purified Pab (1:60 dilution).
Western blot analysis of anti-ASK (C-term) Pab (Cat#AP14025PU-N) in K562 cell line lysates (35ug/lane). ASK(arrow) was detected using the purified Pab (1:60 dilution).
Western Blot analysis of DBF4 expression in transfected 293T cell line (H00010926-T03) by DBF4 MaxPab polyclonal antibody.Lane 1: DBF4 transfected lysate(76.90 KDa).Lane 2: Non-transfected lysate.
Western Blot: DBF4 Lysate [NBL1-09726] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for DBF4
Western Blot: Dbf4 Antibody [NBP1-19618] - extracts from NIH-3T3 treated with H2O2 100uM 30'.
Western blot (WB) analysis of Dbf4 antibody (Cat.-No.: AP20463PU-N) in extracts from NIH-3T3 treated with H2O2 100uM 30'.
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13076-1-AP(DBF4 antibody) at dilution of 1:300
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13076-1-AP(DBF4 antibody) at dilution of 1:300
AP31104PU-N DBF4 antibody staining of Mouse Testis lysate at 1 µg/ml (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: DBF4 Antibody [NBP1-78282] - (1ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemical analysis of DBF4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of DBF4 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of DBF4 expression in Jurkat (A); HepG2 (B); SW480 (C) whole cell lysates.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA311409 | DBF4 / ASK antibody |
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| Goat Anti-Dbf4 (mouse) Antibody | |||||||
 |
Goat | Aff - Purified | Ms, Rt | E, WB |
0.1 mg /
€325.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA315124 | DBF4 / ASK antibody |
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| Rabbit polyclonal anti-DBF4 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP20463PU-N | DBF4 / ASK antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms | P, WB |
0.1 mg /
€350.00
|
||
| Acris Antibodies GmbH | |||||||
| C11821-1 | DBF4 / ASK antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms | E, WB |
50 µg /
€230.00
|
||
| Assay Biotech | |||||||
| C11821-2 | DBF4 / ASK antibody |
||||||
|
Rabbit | Aff - Purified | Hu, Ms | E, WB |
0.1 mg /
€330.00
|
||
| Assay Biotech | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN206120 | DBF4 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN206120BN | DBF4 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN206120LP | DBF4 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN206120RB | DBF4 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN304287 | Dbf4 - mouse gene knockout kit via CRISPR |
||
|
Mouse | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN304287BN | Dbf4 - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN304287LP | Dbf4 - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN304287RB | Dbf4 - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Lysates
| Catalog No. | ||
|---|---|---|
| LY402007 | DBF4 overexpression lysate |
|
|
0.1 mg /
€280.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC206120L2V | Lenti ORF particles, DBF4 (mGFP-tagged) - Human DBF4 homolog (S. cerevisiae) (DBF4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,050.00
|
|
| OriGene Technologies, Inc. | ||
| RC206120L1V | Lenti ORF particles, DBF4 (Myc-DDK tagged) - Human DBF4 homolog (S. cerevisiae) (DBF4) , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€940.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR222369L2V | Lenti ORF particles, Dbf4 (GFP-tagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,340.00
|
|
| OriGene Technologies, Inc. | ||
| MR222368L2V | Lenti ORF particles, Dbf4 (GFP-tagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,340.00
|
|
| OriGene Technologies, Inc. | ||
| MR222369L1V | Lenti ORF particles, Dbf4 (Myc-DDK-tagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,200.00
|
|
| OriGene Technologies, Inc. | ||
| MR222368L1V | Lenti ORF particles, Dbf4 (Myc-DDK-tagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,200.00
|
|
| OriGene Technologies, Inc. | ||
