DBI / ACBP antibody

Principal name

DBI / ACBP antibody

Alternative names for DBI / ACBP antibody

Diazepam-binding inhibitor, Endozepine, Acyl-CoA-binding protein

SwissProt ID

P07107 (Bovin), P07108 (Human), P11030 (Rat), P31786 (Mouse)

Gene ID

1622 (DBI)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine), Rb (Rabbit), Can (Canine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Frozen Sections (C), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of DBI / ACBP antibody

DBI is diazepam binding inhibitor. The protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. DBI is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene. This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene.

General readings

Folli F., et al. (2010) PLoS One. 5(3):e9923.
Manjithaya R., et al. (2010) J Cell Biol. 188(4):537-46.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DBI / ACBP antibody

WB Suggested Anti-DBI Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

DBI / ACBP antibody

Western blot analysis of extracts of various cell lines, using DBI antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

DBI / ACBP antibody

DBI polyclonal antibody (A01), Lot # 060613JCS1 Western Blot analysis of DBI expression in SJCRH30 ( Cat # L027V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

DBI / ACBP antibody

Western Blot analysis of DBI expression in transfected 293T cell line (H00001622-T02) by DBI MaxPab polyclonal antibody.

Lane 1: DBI transfected lysate(11.80 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

DBI / ACBP antibody

Immunoprecipitation analysis of HepG2 cell lysates. Lane 1 : Input and lane 2 : Precipitates. Immunoblotting with DBI monoclonal antibody, clone 20B10 ( Cat # MAB0720 ). Mouse IgG1 20B10 Purified Hu E, IP
0.1 ml / €390.00
  Abnova Taiwan Corp.

DBI / ACBP antibody

Immunoblot analysis of HepG2 cell lysate. Mouse IgG2b 27C9 Purified Hu C, E, IP, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

DBI / ACBP antibody

Immunoblot analysis of U87 MG cell lysate. Rabbit Purified Hu IP, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

DBI / ACBP antibody

Human Brain; Immunohistochemistry with Brain, cerebellum tissue at an antibody concentration of 5ug/ml using anti-DBI antibody (ARP33135_P050); DBI antibody - N-terminal region (ARP33135_P050) in Human Brain cells using Immunohistochemistry Rabbit Aff - Purified Bov, Hu, Ms, Por, Rb, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

DBI / ACBP antibody

Western Blot: diazepam binding inhibitor Antibody [NBP1-54806] - This Anti-DBI antibody was used in Western Blot of Fetal Liver tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu P, WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DBI / ACBP (transcript variant 1)

DBI / ACBP Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

DBI / ACBP (1-87, His-tag)

DBI / ACBP Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €670.00
  Acris Antibodies GmbH

DBI / ACBP (1-87, His-tag)

DBI / ACBP Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €250.00
  Acris Antibodies GmbH

DBI / ACBP

DBI / ACBP Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

DBI / ACBP

DBI / ACBP Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

DBI / ACBP

DBI / ACBP Human
2 µg / €300.00
  Abnova Taiwan Corp.

DBI / ACBP

DBI / ACBP Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

DBI / ACBP

DBI / ACBP Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

DBI / ACBP

SDS-Page: Diazepam Binding Inhibitor Protein [NBP1-44453] - ACBP, 12.2 kDa (107aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE Human Purified
0.1 mg / €340.00
  Novus Biologicals Inc.

DBI / ACBP

DBI / ACBP Human Purified
0.1 mg / €340.00
  Novus Biologicals Inc.

DBI / ACBP

DBI / ACBP Human Purified
0.5 mg / €760.00
  Novus Biologicals Inc.

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