DCI antibody

Principal name

DCI antibody

Alternative names for DCI antibody

Dodecenoyl-CoA isomerase, Delta(3), Delta(2)-enoyl-CoA isomerase, 3,2-trans-enoyl-CoA isomerase mitochondrial

SwissProt ID

P23965 (Rat), P42125 (Mouse), P42126 (Human)

Gene ID

1632 (ECI1)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of DCI antibody

ECI1/DCI(Dodecenoyl-CoA Delta Isomerase), also known as 3,2-trans-enoyl-CoA isomerase, is an enzyme that catalyzes the conversion of cis-or trans-double bonds of fatty acids at gamma-carbon(position 3) to trans double bonds at beta-carbon (position 2). It plays a particularly important role in the metabolism of unsaturated fatty acids. All classes of enoyl-CoA isomerases belong to a family of enzymes, the hydratase/isomerase or crotonase superfamily, and when examined with x-ray crystallography, exhibit a common structural feature of the family, the N-terminal core with a spiral fold composed of four turns, each turn consisting of two beta-sheets and one alpha-helix. Dodecenoyl-CoA Delta Isomerase is involved in the beta-oxidation, one of the most frequently used pathways in fatty acid degradation, of unsaturated fatty acids with double bonds at odd-numbered carbon positions. It does so by shifting the position of the double bonds in the acyl-CoA intermediates and converting 3-cis or trans-enoyl-CoA to 2-trans-enoyl-CoA.

General readings

1. Janssen, U., Fink, T., Lichter, P., Stoffel, W.Human mitochondrial 3,2-trans-enoyl-CoA isomerase
(DCI): gene structure and localization to chromosome 16p13.3.Genomics 23: 223-228, 1994.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DCI antibody

  Anti-ECI1 Rabbit Polyclonal Antibody  
Predicted band size: 33 kDa . Positive control: RT-4 and U-251MG cell, liver and tonail tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: RT-4 cell lysate Lane2: U-251MG cell lysate Lane3: Liver tissue lysate Lane4: Tonail tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DCI antibody

  ECI1 Rabbit Polyclonal Antibody  
Western blot analysis of extracts from COS-7 cells, treated with UV (15mins), using IGF2R (Phospho-Ser2409) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

DCI antibody

  Rabbit Polyclonal Anti-DCI Antibody  
WB Suggested Anti-DCI Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human brain Rabbit IgG Purified Bov, Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

DCI (C-term) antibody

(LEFT) Western blot analysis of DCI Antibody (C-term) (AP17983PU-N) in MCF-7 cell line lysates (35ug/lane). DCI (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of DCI Antibody (C-term) (AP17983PU-N) in mouse heart tissue lysates (35ug/lane). DCI (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

ECI1 - human gene knockout kit via CRISPR

ECI1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ECI1 - human gene knockout kit via CRISPR

ECI1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ECI1 - human gene knockout kit via CRISPR

ECI1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ECI1 - human gene knockout kit via CRISPR

ECI1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DCI (42-302, His-tag)

DCI Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €730.00
  Acris Antibodies GmbH

DCI (42-302, His-tag)

DCI Human Purified > 90 % by SDS - PAGE E. coli
20 µg / €300.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

ECI1 overexpression lysate

ECI1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, ECI1 (mGFP-tagged)-Human enoyl-CoA delta isomerase 1 (ECI1), nuclear gene encoding mitochondrial protein, transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, ECI1 (mGFP-tagged)-Human enoyl-CoA delta isomerase 1 (ECI1), nuclear gene encoding mitochondrial protein, transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.

Lenti ORF particles, ECI1 (mGFP-tagged) - Human enoyl-CoA delta isomerase 1 (ECI1), nuclear gene encoding mitochondrial protein, transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, ECI1 (mGFP-tagged) - Human enoyl-CoA delta isomerase 1 (ECI1), nuclear gene encoding mitochondrial protein, transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.

Lenti ORF particles, ECI1 (Myc-DDK-tagged)-Human enoyl-CoA delta isomerase 1 (ECI1), nuclear gene encoding mitochondrial protein, transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, ECI1 (Myc-DDK-tagged)-Human enoyl-CoA delta isomerase 1 (ECI1), nuclear gene encoding mitochondrial protein, transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €860.00
  OriGene Technologies, Inc.

Lenti ORF particles, ECI1 (Myc-DDK tagged) - Human enoyl-CoA delta isomerase 1 (ECI1), nuclear gene encoding mitochondrial protein, transcript variant 2 , 200 uL, >10^7 TU/mL

Lenti ORF particles, ECI1 (Myc-DDK tagged) - Human enoyl-CoA delta isomerase 1 (ECI1), nuclear gene encoding mitochondrial protein, transcript variant 2 , 200 uL, >10^7 TU/mL  
200 µl / €860.00
  OriGene Technologies, Inc.

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