DCI antibody

Principal name

DCI antibody

Alternative names for DCI antibody

Dodecenoyl-CoA isomerase, Delta(3), Delta(2)-enoyl-CoA isomerase, 3,2-trans-enoyl-CoA isomerase mitochondrial

SwissProt ID

P23965 (Rat), P42125 (Mouse), P42126 (Human)

Gene ID

1632 (ECI1)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of DCI antibody

ECI1/DCI(Dodecenoyl-CoA Delta Isomerase), also known as 3,2-trans-enoyl-CoA isomerase, is an enzyme that catalyzes the conversion of cis-or trans-double bonds of fatty acids at gamma-carbon(position 3) to trans double bonds at beta-carbon (position 2). It plays a particularly important role in the metabolism of unsaturated fatty acids. All classes of enoyl-CoA isomerases belong to a family of enzymes, the hydratase/isomerase or crotonase superfamily, and when examined with x-ray crystallography, exhibit a common structural feature of the family, the N-terminal core with a spiral fold composed of four turns, each turn consisting of two beta-sheets and one alpha-helix. Dodecenoyl-CoA Delta Isomerase is involved in the beta-oxidation, one of the most frequently used pathways in fatty acid degradation, of unsaturated fatty acids with double bonds at odd-numbered carbon positions. It does so by shifting the position of the double bonds in the acyl-CoA intermediates and converting 3-cis or trans-enoyl-CoA to 2-trans-enoyl-CoA.

General readings

1. Janssen, U., Fink, T., Lichter, P., Stoffel, W.Human mitochondrial 3,2-trans-enoyl-CoA isomerase
(DCI): gene structure and localization to chromosome 16p13.3.Genomics 23: 223-228, 1994.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DCI antibody

  Anti-ECI1 Rabbit Polyclonal Antibody  
Predicted band size: 33 kDa . Positive control: RT-4 and U-251MG cell, liver and tonail tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: RT-4 cell lysate Lane2: U-251MG cell lysate Lane3: Liver tissue lysate Lane4: Tonail tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DCI antibody

  ECI1 Rabbit Polyclonal Antibody  
Western blot analysis of extracts from COS-7 cells, treated with UV (15mins), using IGF2R (Phospho-Ser2409) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

DCI antibody

  Rabbit Polyclonal Anti-DCI Antibody  
WB Suggested Anti-DCI Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human brain Rabbit IgG Purified Bov, Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

DCI (C-term) antibody

(LEFT) Western blot analysis of DCI Antibody (C-term) (AP17983PU-N) in MCF-7 cell line lysates (35ug/lane). DCI (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of DCI Antibody (C-term) (AP17983PU-N) in mouse heart tissue lysates (35ug/lane). DCI (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DCI antibody

DCI polyclonal antibody (A01), Lot # 051220JC01 Western Blot analysis of DCI expression in HeLa ( Cat # L013V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

DCI antibody

DCI MaxPab polyclonal antibody. Western Blot analysis of DCI expression in human liver. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

DCI antibody

DCI Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

DCI antibody

Human Brain; WB Suggested Anti-DCI Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human brain; DCI antibody - N-terminal region (ARP48556_P050) in Human Brain cells using Western Blot Rabbit Aff - Purified Bov, Hu WB
50 µg / €350.00
  AVIVA Systems Biology

DCI antibody

DCI Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

CRISPR

Catalog No. Species Donor Vector  

ECI1 - human gene knockout kit via CRISPR

ECI1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ECI1 - human gene knockout kit via CRISPR

ECI1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ECI1 - human gene knockout kit via CRISPR

ECI1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ECI1 - human gene knockout kit via CRISPR

ECI1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DCI (42-302, His-tag)

DCI Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €730.00
  Acris Antibodies GmbH

DCI (42-302, His-tag)

DCI Human Purified > 90 % by SDS - PAGE E. coli
20 µg / €300.00
  Acris Antibodies GmbH

DCI

DCI Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

DCI

DCI Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

DCI

DCI Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

DCI

DCI Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

DCI 293T Cell Transient Overexpression Lysate(Denatured)

DCI 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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