DCP1A antibody

Principal name

DCP1A antibody

Alternative names for DCP1A antibody

SMIF, mRNA-decapping enzyme 1A, Transcription factor SMIF, Smad4-interacting transcriptional co-activator

SwissProt ID

Q91YD3 (Mouse), Q9NPI6 (Human)

Gene ID

55802 (DCP1A), 1055802 (Q8CRC6)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of DCP1A antibody

Signal transduction of TGF-beta superfamily members is regulated by Smad proteins. In particular, Smads influence specific gene transcription by relaying signals from the cell membrane to the nucleus. Smad4 plays an essential role in TGF-beta-induced transcriptional activation wherein phosphorylated receptor-associated Smads associate with Smad4. Furthermore, SMIF (Smad4- intereacting protein) and Smad4 complex with TGF-beta and BMP4. An increase in Smad4 concentration increases the translocation of this complex to the nucleus. SMIF and Smad4 interact directly through a EVH1/WH1 domain on SMIF and a proline-rich activation domain on Smad4. Smad4 is essential to nuclear translocation of SMIF as deletion of the Smad4-interacting domain (located in the N-terminal 100 amino acids) of SMIF eliminates TGF-beta-induced nuclear translocation of SMIF (1). The human SMIF gene is ubiquitously expressed and encodes a protein with a relative molecular mass of 70 kDa.

General readings

Cougot N, Babajko S, Seraphin B. Cytoplasmic foci are sites of mRNA decay in human cells. J Cell Biol. 2004 Apr;165(1):31-40. Epub 2004 Apr 5. PMID: 15067023

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DCP1A antibody

TA303086 (2µg/ml) staining of A431 cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DCP1A antibody

Western blot analysis of HeLa cell lysates with anti-Dcp1a antibody (1:500). Rabbit Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DCP1A antibody

Western blot analysis of extracts from Jurkat cells, using DCP1A antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DCP1A antibody

WB Suggested Anti-DCP1A Antibody Titration: 2.5 ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysateDCP1A is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DCP1A antibody

WB Suggested Anti-DCP1A Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate; DCP1A is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DCP1A antibody

Western blot analysis on Jurkat cell lysate using DCP1A Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DCP1A antibody

Western blot (WB) analysis of SMIF antibody (Cat.-No.: AP06755PU-N) in extracts from Jurkat cells. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

DCP1A antibody

DCP1A polyclonal antibody (A01), Lot # 060428JCS1. Western Blot analysis of DCP1A expression in Raw 264.7. Mouse Serum Hu, Ms E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

DCP1A antibody

DCP1A MaxPab polyclonal antibody. Western Blot analysis of DCP1A expression in rat brain. Mouse Purified Hu, Rt E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

DCP1A antibody

Immunoprecipitation of DCP1A transfected lysate using anti-DCP1A MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with DCP1A MaxPab mouse polyclonal antibody (B01) (H00055802-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

DCP1A antibody

Western Blot analysis of DCP1A expression in transfected 293T cell line by DCP1A monoclonal antibody (M01), clone 2D12.

Lane 1: DCP1A transfected lysate(63.3 KDa).
Lane 2: Non-transfected lysate. Mouse IgG1 2D12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DCP1A antibody

DCP1A monoclonal antibody (M02), clone 2F11. Western Blot analysis of DCP1A expression in IMR-32 ( Cat # L008V1 ). Mouse IgG1 2F11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DCP1A antibody

Immunofluorescence of monoclonal antibody to DCP1A on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 3G4 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DCP1A antibody

DCP1A monoclonal antibody (M07), clone 2G10. Western Blot analysis of DCP1A expression in IMR-32 ( Cat # L008V1 ). Mouse IgG1 2G10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DCP1A antibody

DCP1A polyclonal antibody ( Cat # PAB6970 ) ( 2 µg/ml ) staining of A-431 cell lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

DCP1A antibody

Western blot analysis of extracts from Jurkat cells, using DCP1A Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

DCP1A antibody

Western blot analysis of extracts from Jurkat cells, using DCP1A Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

DCP1A antibody

Human Jurkat; WB Suggested Anti-DCP1A Antibody Titration: 2.5ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate. DCP1A is supported by BioGPS gene expression data to be expressed in Jurkat.; DCP1A antibody - N-terminal region (ARP34582_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

DCP1A antibody

Human Jurkat; WB Suggested Anti-DCP1A Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate. DCP1A is supported by BioGPS gene expression data to be expressed in Jurkat.; DCP1A antibody - N-terminal region (ARP39353_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

DCP1A antibody

Western Blot: DCP1A Antibody [NB100-2830] - NB100-2830 (2ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

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