DDB1 / XAP1 antibody

Principal name

DDB1 / XAP1 antibody

Alternative names for DDB1 / XAP1 antibody

DDBa, UV-DDB 1, XAP-1, XPCe, XPE-BF, DNA damage-binding protein 1, Damage-specific DNA-binding protein 1, DNA damage-binding protein a, UV-damaged DNA-binding factor, HBV X-associated protein 1, Xeroderma pigmentosum group E-complementing protein

SwissProt ID

A1A4K3 (Bovin), Q16531 (Human), Q3U1J4 (Mouse), Q805F9 (Chick), Q9ESW0 (Rat)

Gene ID

100290337 (LOC100290337), 1642 (DDB1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of DDB1 / XAP1 antibody

DDB1 is also known as damage-specific DNA binding protein 1, DDB p127 subunit, DDBa, UV damaged DNA-binding protein 1, UV-DDB 1, Xeroderma pigmentosum group E complementing protein, XPCe, X-associated protein 1 and XAP-1. The DDB1 gene encodes the large subunit (p127) of DNA damage-binding protein, which is a heterodimer, composed of a large and a small subunit (p48 DDB2). This nuclear protein functions in nucleotide-excision repair resulting from UV-damaged DNA by binding to pyrimidine dimers. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). XP-E is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients.

General readings

1. Li T, Robert EI, van Breugel PC, Strubin M, Zheng N.
A promiscuous alpha-helical motif anchors viral hijackers and substrate receptors to the CUL4-DDB1 ubiquitin ligase machinery. Nat Struct Mol Biol. 2010 Jan;17(1):105-11.
PMID: 19966799
2. Westbrook TF, Martin ES, Schlabach MR, Leng Y, Liang AC, Feng B, Zhao JJ, Roberts TM, Mandel G, Hannon GJ, Depinho RA, Chin L, Elledge SJ. A genetic screen for candidate tumor suppressors identifies REST. Cell. 2005 Jun 17;121(6):837-48. PMID: 15960972
3. Leung-Pineda V, Huh J, Piwnica-Worms H. DDB1 targets Chk1 to the Cul4 E3 ligase complex in normal cycling cells and in cells experiencing replication stress Cancer Res. 2009 Mar 15;69(6):2630-7. PMID: 19276361
4. Alekseev S, Luijsterburg MS, Pines A, Geverts B, Mari PO, Giglia-Mari G, Lans H, Houtsmuller AB, Mullenders LH, Hoeijmakers JH, Vermeulen W. Cellular concentrations of DDB2 regulate dynamic binding of DDB1 at UV-induced DNA damage. Mol Cell Biol. 2008 Dec;28(24):7402-13. PMID: 18936169
5. Luijsterburg MS, Goedhart J, Moser J, Kool H, Geverts B, Houtsmuller AB, Mullenders LH, Vermeulen W, van Driel R.
Dynamic in vivo interaction of DDB2 E3 ubiquitin ligase with UV-damaged DNA is independent of damage-recognition protein XPC. J Cell Sci. 2007 Aug 1;120(Pt 15):2706-16.
PMID: 17635991

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DDB1 / XAP1 antibody

Sample (50 ug of whole cell lysate). A: Mouse brain. 5% SDS PAGE. TA308812 diluted at 1:5000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DDB1 / XAP1 antibody

Immunohistochemistry: DDB1 Antibody [NBP1-33061] - Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using NBP1-33061. Rabbit IgG Hu, Ms ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

DDB1 / XAP1 (C-term) antibody

TA302707 (1 µg/ml) staining of NSO lysate (1E5 cells per lane).  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
20 µg / €200.00
  Acris Antibodies GmbH

DDB1 / XAP1 (C-term) antibody

TA302707 (1 µg/ml) staining of NSO lysate (1E5 cells per lane).  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DDB1 / XAP1 antibody

Predicted band size: 127kDa. Positive control: Human fetal small intestine, human liver cancer and human lymphoma tissue lysate,293T and A549 cell lysate . Recommended dilution: 1/200-500 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DDB1 / XAP1 antibody

Predicted band size: 127kDa. Positive control: MCF-7, Hela and HepG2 cell lysate . Recommended dilution: 1/200-1000 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DDB1 / XAP1 antibody

Western blot detection of DDB1 in Hela,MCF7,COS7,C6 and 3T3 cell lysates using DDB1 mouse mAb (1:1000 diluted),with Super ECL.Predicted band size:127KDa.Observed band size:127KDa. Mouse IgG2b 2D6-B5-E6 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DDB1 / XAP1 (198-213) antibody

Brain, cortex: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Hu E, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

DDB1 / XAP1 antibody

Figure 1. Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid).
Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins.
Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins
by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in
ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier
is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin.
Data contributed by S. Jentsch, see references above. Rabbit Serum Hu, Ms E, IP, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

DDB1 / XAP1 antibody

Western blot analysis of DDB1 in NSO cell lysate with SP1341P. Goat Purified Hu WB
0.1 mg / €550.00
  Acris Antibodies GmbH

DDB1 / XAP1 antibody

DDB1 polyclonal antibody (A01), Lot # 060109JC01 Western Blot analysis of DDB1 expression in SJCRH30 ( Cat # L027V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

DDB1 / XAP1 antibody

DDB1 / XAP1 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

DDB1 / XAP1 antibody

Immunoperoxidase of monoclonal antibody to DDB1 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 3 ug/ml] Mouse IgG2a 4C5 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DDB1 / XAP1 antibody

DDB1 polyclonal antibody ( Cat # PAB6008 ) staining ( 1 µg/ml ) of NSO lysate ( 1E5 cells per lane ) . Detected by western blot using chemiluminescence. Goat Hu, Ms E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

DDB1 / XAP1 antibody

NB 100-777 (1 ug/ml) staining of NSO lysate (1E5 cells per lane). Detected by western blot using chemiluminescence Goat IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

DDB1 / XAP1 antibody

DDB1 / XAP1 Rabbit Hu E, IP, P, WB
50 µl / €480.00
  Novus Biologicals Inc.

DDB1 / XAP1 antibody

DDB1 / XAP1 Rabbit Purified Hu E, WB
0.1 ml / €510.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DDB1 / XAP1

DDB1 / XAP1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

DDB1 / XAP1

DDB1 / XAP1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

DDB1 / XAP1

DDB1 / XAP1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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