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DDB2 antibody

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Principal name

DDB2 antibody

Alternative names for DDB2 antibody

DNA damage-binding protein 2, UV-DDB2, DDB p48 subunit, DDBb, UV-damaged DNA-binding protein 2, Damage-specific DNA-binding protein 2

SwissProt ID

Q0VBY8 (Bovin), Q5ZJL7 (Chick), Q92466 (Human), Q99J79 (Mouse)

Gene ID

1643 (DDB2), 107986 (Ddb2)

Ncbi ID

NP_000098.1, 9606, NP_000098, NM_000107, NP_082395.2

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (detection) (E(detection))

Background of DDB2 antibody

Kit Component:
- KN304359G1, Ddb2 gRNA vector 1 in pCas-Guide vector
- KN304359G2, Ddb2 gRNA vector 2 in pCas-Guide vector
- KN304359D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Jentsch S, Pyrowolakis G. (2000) Ubiquitin and its kin: how close are the family ties? Trends Cell Biol. 10(8):335-42.
Inoki,T., Yamagami,S., Inoki,Y., Tsuru,T., Hamamoto,T., Kagawa,Y., Mori,T. and Endo,H. (2004) Human DDB2 splicing variants are dominant negative inhibitors of UV-damaged DNA repair. Biochem. Biophys. Res. Commun. 314 (4), 1036-1043.
Fitch,M.E., Nakajima,S., Yasui,A. and Ford,J.M. (2003) In vivo recruitment of XPC to UV-induced cyclobutane pyrimidine dimers by the DDB2 gene product. J. Biol. Chem. 278 (47), 46906-46910.
Itoh,T., O Shea,C. and Linn,S. (2003) Impaired regulation of tumor suppressor p53 caused by mutations in the xeroderma pigmentosum DDB2 gene: mutual regulatory interactions between p48(DDB2) and p53. Mol. Cell. Biol. 23 (21), 7540-7553.
Rapic-Otrin,V., Navazza,V., Nardo,T., Botta,E., McLenigan,M., Bisi,D.C., Levine,A.S. and Stefanini,M. (2003) True XP group E patients have a defective UV-damaged DNA binding protein complex and mutations in DDB2 which reveal the functional domains of its p48 product. Hum.
Mol. Genet. 12 (13), 1507-1522.
Groisman,R., Polanowska,J., Kuraoka,I., Sawada,J., Saijo,M., Drapkin,R., Kisselev,A.F., Tanaka,K. and Nakatani,Y. (2003) The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage. Cell 113(3), 357-367.

R1517 - DDB2
Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid).Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins.Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullinsby RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin.Data contributed by S. Jentsch, see references above.
H00001643-D01P - DDB2
Western Blot analysis of DDB2 expression in transfected 293T cell line (H00001643-T04) by DDB2 MaxPab polyclonal antibody.Lane 1: DDB2 transfected lysate(47.90 KDa).Lane 2: Non-transfected lysate.
ARP60163_P050 - DDB2
Human Placenta; WB Suggested Anti-DDB2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Placenta; DDB2 antibody - C-terminal region (ARP60163_P050) in Human Placenta cells using Western Blot
ARP60163_P050 - DDB2
Hum. Fetal Brain; Host:Rabbit. Target Name:DDB2. Sample Tissue:Human Fetal Brain. Antibody Dilution: 1.0ug/ml.; DDB2 antibody - C-terminal region (ARP60163_P050) in Hum. Fetal Brain cells using Western Blot
TA327198 - DDB2
Western blot analysis of extracts of MCF-7 cell line, using DDB2 antibody.
TA327198 - DDB2
Immunohistochemistry of paraffin-embedded human kidney using DDB2 antibody at dilution of 1:200 (200x lens).
TA327198 - DDB2
Immunohistochemistry of paraffin-embedded human kidney cancer using DDB2 antibody at dilution of 1:200 (200x lens).
TA327198 - DDB2
Immunohistochemistry of paraffin-embedded human kidney cancer using DDB2 antibody at dilution of 1:200 (400x lens).
TA327198 - DDB2
Immunofluorescence analysis of MCF7 cell using DDB2 antibody. Blue: DAPI for nuclear staining.
TA342865 - DDB2
WB Suggested Anti-DDB2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Placenta
TA342865 - DDB2
Host: Rabbit; Target Name: DDB2; Sample Tissue: Human Fetal Brain; Antibody Dilution: 1.0 ug/ml
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DDB2 antibody
Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S. Jentsch, see references above. Rabbit Serum Hu, Ms E, IP, WB
0.1 ml / €450.00
  Acris Antibodies GmbH
 

Published recently!

DDB2 antibody
  DDB2 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of MCF-7 cell line, using DDB2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.
+4 additional images

Highly validated antibody!

DDB2 antibody
  Rabbit Polyclonal Anti-DDB2 Antibody  
WB Suggested Anti-DDB2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.
+1 additional image

CRISPR

Catalog No. Species Donor Vector  

DDB2 - human gene knockout kit via CRISPR
DDB2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

DDB2 - human gene knockout kit via CRISPR
DDB2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

DDB2 - human gene knockout kit via CRISPR
DDB2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

DDB2 - human gene knockout kit via CRISPR
DDB2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ddb2 - mouse gene knockout kit via CRISPR
Ddb2 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Ddb2 - mouse gene knockout kit via CRISPR
Ddb2 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ddb2 - mouse gene knockout kit via CRISPR
Ddb2 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ddb2 - mouse gene knockout kit via CRISPR
Ddb2 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

DDB2 overexpression lysate
DDB2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, DDB2 (mGFP-tagged) - Human damage-specific DNA binding protein 2, 48kDa (DDB2), 200 uL, >10^7 TU/mL
Lenti ORF particles, DDB2 (mGFP-tagged) - Human damage-specific DNA binding protein 2, 48kDa (DDB2), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, DDB2 (Myc-DDK tagged) - Human damage-specific DNA binding protein 2, 48kDa (DDB2) , 200 uL, >10^7 TU/mL
Lenti ORF particles, DDB2 (Myc-DDK tagged) - Human damage-specific DNA binding protein 2, 48kDa (DDB2) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.
 

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Target
  1. DDB2 (3)
Reactivity
  1. Human (Hu) (3)
  2. Mouse (Ms) (3)
  3. Rat (Rt) (2)
  4. Bovine (Bov) (1)
  5. Canine (Can) (1)
  6. Equine (Eq) (1)
  7. Guinea Pig (GP) (1)
  8. Porcine (Por) (1)
  9. Rabbit (Rb) (1)
Applications
  1. Western blot / Immunoblot (WB) (2)
  2. Enzyme Immunoassay (E) (1)
  3. Immunoprecipitation (IP) (1)
Presentation
  1. Purified (2)
  2. Serum (1)
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Images
  1. 1 image (3)
  2. 3+ images (1)
  3. 5+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (2)
  2. Immunofluorescence (IF) (1)
  3. IHC (1)
Species (CRISPR)
  1. Human (4)
  2. Mouse (4)
Donor Vector
  1. GFP-Puro (2)
  2. Luciferase-Puro (2)
  3. RFP-BSD (2)
  4. mBFP-Neo (2)
Manufacturer
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