DDB2 antibody

Principal name

DDB2 antibody

Alternative names for DDB2 antibody

DNA damage-binding protein 2, UV-DDB2, DDB p48 subunit, DDBb, UV-damaged DNA-binding protein 2, Damage-specific DNA-binding protein 2

SwissProt ID

Q0VBY8 (Bovin), Q5ZJL7 (Chick), Q92466 (Human), Q99J79 (Mouse)

Gene ID

1643 (DDB2), 107986 (Ddb2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (detection) (E(detection))

Background of DDB2 antibody

Kit Component:
- KN304359G1, Ddb2 gRNA vector 1 in pCas-Guide vector
- KN304359G2, Ddb2 gRNA vector 2 in pCas-Guide vector
- KN304359D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Jentsch S, Pyrowolakis G. (2000) Ubiquitin and its kin: how close are the family ties? Trends Cell Biol. 10(8):335-42.
Inoki,T., Yamagami,S., Inoki,Y., Tsuru,T., Hamamoto,T., Kagawa,Y., Mori,T. and Endo,H. (2004) Human DDB2 splicing variants are dominant negative inhibitors of UV-damaged DNA repair. Biochem. Biophys. Res. Commun. 314 (4), 1036-1043.
Fitch,M.E., Nakajima,S., Yasui,A. and Ford,J.M. (2003) In vivo recruitment of XPC to UV-induced cyclobutane pyrimidine dimers by the DDB2 gene product. J. Biol. Chem. 278 (47), 46906-46910.
Itoh,T., O Shea,C. and Linn,S. (2003) Impaired regulation of tumor suppressor p53 caused by mutations in the xeroderma pigmentosum DDB2 gene: mutual regulatory interactions between p48(DDB2) and p53. Mol. Cell. Biol. 23 (21), 7540-7553.
Rapic-Otrin,V., Navazza,V., Nardo,T., Botta,E., McLenigan,M., Bisi,D.C., Levine,A.S. and Stefanini,M. (2003) True XP group E patients have a defective UV-damaged DNA binding protein complex and mutations in DDB2 which reveal the functional domains of its p48 product. Hum.
Mol. Genet. 12 (13), 1507-1522.
Groisman,R., Polanowska,J., Kuraoka,I., Sawada,J., Saijo,M., Drapkin,R., Kisselev,A.F., Tanaka,K. and Nakatani,Y. (2003) The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage. Cell 113(3), 357-367.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DDB2 antibody

Conjugation  pathways for  ubiquitin and ubiquitin-like modifiers (UBLs).  
Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid).
Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins.
Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins
by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin.
Data contributed by  S. Jentsch, see references above. Rabbit Serum Hu, Ms E, IP, WB
0.1 ml / €450.00
  Acris Antibodies GmbH

DDB2 antibody

Western blot analysis of extracts of MCF-7 cell line, using DDB2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

DDB2 antibody

WB Suggested Anti-DDB2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

DDB2 antibody

Western Blot analysis of DDB2 expression in transfected 293T cell line (H00001643-T04) by DDB2 MaxPab polyclonal antibody.

Lane 1: DDB2 transfected lysate(47.90 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

DDB2 antibody

DDB2 Mouse IgG2b 1F11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DDB2 (C-term) antibody

DDB2 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DDB2

DDB2 Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

DDB2

DDB2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

DDB2

DDB2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

DDB2

DDB2 Human Purified
10 µg / €380.00
  Abnova Taiwan Corp.

DDB2

DDB2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

DDB2

DDB2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

DDB2 overexpression lysate

DDB2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

13 Item(s)

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