DDEFL1 antibody

Principal name

DDEFL1 antibody

Alternative names for DDEFL1 antibody

ASAP3, UPLC1, ACAP4, CENTB6, Development and differentiation-enhancing factor-like 1, Protein up-regulated in liver cancer 1, Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3

SwissProt ID

Q5U464 (Mouse), Q8TDY4 (Human)

Gene ID

55616 (ASAP3), 230837 (Asap3)

Available reactivities

Hst (Hamster), Hu (Human), Ms (Mouse), Rt (Rat), Prim (Primate), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Frozen Sections (C), Enzyme Immunoassay (E)

Background of DDEFL1 antibody

UPLC1 (up-regulated in liver cancer 1), also named DDEFL1 (development and differentiation-enhancing factor-like 1) or ASAP3, is a member of the AZAP family of proteins. These proteins catalyze the hydrolysis of GTP bound to ADP-ribosylation factor (Arf) proteins, thereby causing Arf inactivation. For this reason, the ASAPs are generally called ArfGAPs. The activity of ArfGAPs is dependent on the presence of phosphoinositides and is implicated in cellular processes such as membrane trafficking and remodeling of the actin cytoskeleton. Both ASAP1 and ASAP3 have recently been implicated in the development of human cancers. Abnormal activity of ArfGAPs may lead to the alterations characteristic of malignant cells, such as the high rate of proliferation and invasion. More specifically, ASAP3 has been found to be up-regulated in 80% of the hepatocellular carcinomas examined. Initial biochemical characterization reveals that ASAP3 shows class-specific GAP activity on Arf proteins, preferring Arf5 over Arf1 and Arf6.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DDEFL1 antibody

  Rabbit polyclonal Uplc1/Asap3 antibody  
Western blot using protein A purified anti-UPLC1/ASAP3 antibody shows detection of UPLC1/ASAP3 in NIH/3T3 cells over-expressing the protein. Cell extracts (5 ug) were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with anti-UPLC1/ASAP3 at a 1:10,000 dilution. Personal Communication, Vi Luan HA, CCR-NCI, Bethesda, MD. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DDEFL1 antibody

  Rabbit Polyclonal ACAP4 Antibody  
Immunohistochemistry-Paraffin: ACAP4 Antibody [TA337029] - Analysis of ASAP3 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml. Rabbit Purified Hu, Prim P
0.1 mg / €325.00
  OriGene Technologies, Inc.

DDEFL1 antibody

  Rabbit Polyclonal Anti-ASAP3 Antibody - N-terminal region  
WB Suggested Anti-ASAP3 Antibody Titration: 0.2-1 ug/ml; Positive Control: MCF7 cell lysate Rabbit IgG Purified Bov, Eq, GP, Hu, Ms, Rb, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

DDEFL1 (N-term) antibody

ASAP3 antibody (N-term) (Cat. #AP50266PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ASAP3 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hst, Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DDEFL1 antibody

Western Blot analysis of ASAP3 expression in transfected 293T cell line (H00055616-T01) by ASAP3 MaxPab polyclonal antibody.

Lane 1: DDEFL1 transfected lysate(99.33 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

DDEFL1 antibody

Western blot using ASAP3 polyclonal antibody ( Cat # PAB11289 ) shows detection of ASAP3 in NIH/3T3 cells over-expressing the protein.
Cell extracts (5 µg) were resolved by SDS PAGE.
The membrane was probed with ASAP3 polyclonal antibody at a 1 : 10,000 dilution.
Personal Communication, Vi Luan HA, CCR-NCI, Bethesda, MD. Rabbit Hu, Ms, Rt C, E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

DDEFL1 antibody

Human MCF-7; WB Suggested Anti-ASAP3 Antibody Titration: 0.2-1 ug/ml. Positive Control: MCF7 cell lysate; ASAP3 antibody - N-terminal region (ARP57013_P050) in Human MCF-7 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

ASAP3 - human gene knockout kit via CRISPR

ASAP3 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ASAP3 - human gene knockout kit via CRISPR

ASAP3 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Asap3 - mouse gene knockout kit via CRISPR

Asap3 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Asap3 - mouse gene knockout kit via CRISPR

Asap3 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Asap3 - mouse gene knockout kit via CRISPR

Asap3 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Asap3 - mouse gene knockout kit via CRISPR

Asap3 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DDEFL1 (transcript variant 1)

  Recombinant protein of human ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (ASAP3), transcript variant 1  
DDEFL1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

DDEFL1

DDEFL1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

DDEFL1

DDEFL1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

DDEFL1 293T Cell Transient Overexpression Lysate(Denatured)

DDEFL1 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, ASAP3 (mGFP-tagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (ASAP3), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, ASAP3 (mGFP-tagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (ASAP3), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,880.00
  OriGene Technologies, Inc.

Lenti ORF particles, ASAP3 (Myc-DDK-tagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (ASAP3), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, ASAP3 (Myc-DDK-tagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (ASAP3), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,690.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Asap3 (GFP-tagged) - Mouse ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (Asap3), 200 uL, >10^7 TU/mL

Lenti ORF particles, Asap3 (GFP-tagged) - Mouse ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (Asap3), 200 uL, >10^7 TU/mL  
200 µl / €1,660.00
  OriGene Technologies, Inc.

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