DDX58 antibody

Principal name

DDX58 antibody

Alternative names for DDX58 antibody

RIG-1, RIG-I, DKFZp434J1111, DKFZp686N19181, FLJ13599, DEAD box protein 58, Probable ATP-dependent RNA helicase DDX58, Retinoic acid-inducible gene 1 protein

SwissProt ID

O95786 (Human), Q6Q899 (Mouse), Q9GLV6 (Pig)

Gene ID

23586 (DDX58), 230073 (Ddx58)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Prim (Primate), Eq (Equine)

Available hosts

Rabbit, Mouse, Goat, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C)

Background of DDX58 antibody

Antiviral inte immunity depends on the combition of parallel pathways triggered by virus detecting proteins in the Toll-like receptor (TLR) family and R helicases, such as Rig-I (retinoic acid-inducible gene I) and MDA-5 (melanoma differentiation-associated antigen 5), which promote the transcription of type I interferons (IFN) and antiviral enzymes. TLRs and helicase proteins contain sites that recognize the molecular patterns of different virus types, including D, single-stranded R (ssR), double-stranded R (dsR), and glycoproteins. These antiviral proteins are found in different cell compartments; TLRs (i.e. TLR3, TLR7, TLR8, and TLR9) are expressed on endosomal membranes and helicases are localized to the cytoplasm. Rig-I expression is induced by retinoic acid, LPS, IFN, and viral infection. Both Rig-I and MDA-5 share a DExD/H-box helicase domain that detects viral dsR and two amino-termil caspase recruitment domains (CARD) that are required for triggering downstream sigling. Rig-I binds both dsR and viral ssR that contains a 5'-triphosphate end not seen in host R. Though structurally related, Rig-I and MDA-5 detect a distinct set of viruses . The CARD domain of the helicases, which is sufficient to generate sigling and IFN production, is recruited to the CARD domain of the MAVS/VISA/Cardif/IPS-1 mitochondrial protein, which triggers activation of NF-?B, TBK1/IKKe, and IRF-3/IRF-7.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DDX58 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DDX58 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506141) Mouse IgG2a OTI6C1 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

DDX58 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-DDX58 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506202) Mouse IgG1 OTI8D2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DDX58 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DDX58 mouse monoclonal antibody. (TA506160) Mouse IgG2b OTI7D5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DDX58 antibody

Western blot analysis of RIG-1 in C2C12 cell lysate with RIG-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DDX58 antibody

Predicted band size: 107 kDa . Positive control: Jurkat, HepG2 and MCF7 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Jurkat cell lysate Lane2: HepG2 cell lysate Lane3: MCF7 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DDX58 antibody

Western blot analysis of extracts of various cell lines, using DDX58 antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

DDX58 (C-term) antibody

Western blot analysis of extracts from Raw 264.7 cells starved for 20h (lane 1) and starved for 20h and treated with 1 ug/ml LPS for 9h (lane 2) using anti-RIG-1 antibody (clone SS1A). Rat IgG2a SS1A Purified Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DDX58 antibody

Western Blot: DDX58 Antibody (2M6F10) [TA337138] - Western blot analysis of RIG-I in Jurkat cell lysate in the A) absence and B) presence of immunizing peptide and C) HEK 293 cell lysate using TA337138 at 10 ug/ml. Mouse IgG1 2M6F10 Purified Hu, Ms, Prim C, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DDX58 antibody

WB Suggested Anti-DDX58 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: OVCAR-3 cell lysate Rabbit IgG Purified Eq, Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

DDX58 (C-term) antibody

Western blot analysis of extracts from Raw 264.7 cells starved for 20h (lane 1) and starved for 20h and treated with 1 ug/ml LPS for 9h (lane 2) using anti-RIG-1 antibody (clone SS1A). Rat IgG2a SS1A Purified Ms WB
25 µg / €220.00
  Acris Antibodies GmbH

DDX58 (C-term) antibody

24-hour post infection immunoblots of whole cell lysates from primary murine microglia cells (2x10e6) untreated (0) or exposed to vesicular stomatitis virus at a range of viral particle/cell ratios. Data courtesy of Dr. Samantha Furr, University of North Carolina at Charlotte. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DDX58 antibody

Western blot analysis of DDX58 Antibody Cat.-No AP11678PU-N in 293 cell line lysates (35ug/lane). DDX58 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DDX58 antibody

Spleen, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bov, Elephant, Hu, Mky, Ms, Prim, Rt P
50 µg / €470.00
  Acris Antibodies GmbH

DDX58 antibody

Spleen, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Hu, Mky, Prim P
50 µg / €470.00
  Acris Antibodies GmbH

DDX58 antibody

Western Blot analysis of DDX58 expression in transfected 293T cell line (H00023586-T01) by DDX58 MaxPab polyclonal antibody.

Lane 1: DDX58 transfected lysate(101.75 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

DDX58 antibody

DDX58 Rabbit Hu, Ms E
0.4 ml / €390.00
  Abnova Taiwan Corp.

DDX58 antibody

DDX58 Rabbit Hu E
0.4 ml / €390.00
  Abnova Taiwan Corp.

DDX58 antibody

Immunohistochemistry of DDX58 in human heart tissue with DDX58 polyclonal antibody ( Cat # PAB12973 ) at 5 µg/ml. Rabbit Purified Hu, Ms, Rt P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

DDX58 antibody

Human OVCAR-3; WB Suggested Anti-DDX58 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: OVCAR-3 cell lysate; DDX58 antibody - middle region (ARP36416_P050) in Human OVCAR-3 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

DDX58 antibody

DDX58 Goat Aff - Purified Hu P
50 µg / €480.00
  Novus Biologicals Inc.

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