DDX6 antibody

Principal name

DDX6 antibody

Alternative names for DDX6 antibody

DDX-6, HLR2, RCK, Oncogene RCK, DEAD box protein 6, ATP-dependent RNA helicase DDX6, ATP-dependent RNA helicase p54

SwissProt ID

P26196 (Human), P54823 (Mouse), P54824 (Xenla), Q5ZKB9 (Chick), Q9WTM2 (Cavpo)

Gene ID

1656 (DDX6), 13209 (Ddx6)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), African clawed frog, Eq (Equine), GP (Guinea Pig), Gt (Goat), Por (Porcine), Rb (Rabbit), Ye (Yeast), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of DDX6 antibody

Kit Component:
- KN304411G1, Ddx6 gRNA vector 1 in pCas-Guide vector
- KN304411G2, Ddx6 gRNA vector 2 in pCas-Guide vector
- KN304411D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Broytman, O., et al. Neurobiol. Aging 30(12):1962-1974 (2009).
Han, J.W., et al. Nat. Genet. 41(11):1234-1237 (2009).
Minshall, N., et al. Mol. Biol. Cell 20(9):2464-2472 (2009).
Tritschler, F., et al. Mol. Cell 33(5):661-668 (2009).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DDX6 antibody

  Goat Polyclonal Antibody against DEAD-box protein 6  
TA302462 (0.3 ug/ml) staining of Daudi (A), Jurkat (B) and HepG2 (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DDX6 antibody

  Rabbit Polyclonal Anti-DDX6 Antibody  
WB Suggested Anti-DDX6 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: OVCAR-3 cell lysateDDX6 is supported by BioGPS gene expression data to be expressed in OVCAR3 Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

DDX6 antibody

  Rabbit Polyclonal Anti-DDX6 Antibody  
WB Suggested Anti-DDX6 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DDX6 antibody

  Mouse Monoclonal anti-DDX6 Antibody  
Total cell lysate from Jurkat cells (lane 1), HeLa cells (lane 2), and mouse NIH-3T3 cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-DDX6 clone (11G10B39) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. Mouse 11G10B39 Purified Hu, Ms ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DDX6 antibody

  DDX6 mouse monoclonal antibody, clone OTI2D6 (formerly 2D6)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-DDX6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804323) Mouse IgG2a OTI2D6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DDX6 antibody

  Purified DDX6 mouse monoclonal antibody, clone OTI3B7 (formerly 3B7)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX6 (RC209431, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX6. Mouse IgG2b OTI3B7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DDX6 antibody

  Purified DDX6 mouse monoclonal antibody, clone OTI3E2 (formerly 3E2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX6 (RC209431, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX6. Mouse IgG1 OTI3E2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DDX6 (Center) antibody

Formalin fixed, paraffin embedded breast carcinoma reacted with DDX6 Antibody (Center) Cat.-No AP51226PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

DDX6 - human gene knockout kit via CRISPR

DDX6 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

DDX6 - human gene knockout kit via CRISPR

DDX6 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

DDX6 - human gene knockout kit via CRISPR

DDX6 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

DDX6 - human gene knockout kit via CRISPR

DDX6 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ddx6 - mouse gene knockout kit via CRISPR

Ddx6 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Ddx6 - mouse gene knockout kit via CRISPR

Ddx6 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ddx6 - mouse gene knockout kit via CRISPR

Ddx6 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ddx6 - mouse gene knockout kit via CRISPR

Ddx6 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DDX6

  Recombinant protein of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 (DDX6)  
DDX6 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, DDX6 (mGFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 (DDX6), 200 uL, >10^7 TU/mL

Lenti ORF particles, DDX6 (mGFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 (DDX6), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, DDX6 (Myc-DDK tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 (DDX6) , 200 uL, >10^7 TU/mL

Lenti ORF particles, DDX6 (Myc-DDK tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 (DDX6) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Ddx6 (GFP-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 (Ddx6), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, Ddx6 (GFP-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 (Ddx6), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,100.00
  OriGene Technologies, Inc.

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