DEK antibody

Principal name

DEK antibody

Alternative names for DEK antibody

Protein DEK

SwissProt ID

P35659 (Human), Q6AXS3 (Rat), Q7TNV0 (Mouse)

Gene ID

7913 (DEK), 110052 (Dek), 306817

Available reactivities

Bov (Bovine), Hu (Human), Rt (Rat), Chk (Chicken), Ms (Mouse), Fe (Feline), Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P)

Background of DEK antibody

Eukaryotic mRNAs exist in vivo as ribonucleoprotein particles (mRNPs). The protein components of mRNPs have important functions in mRNA metabolism, including effects on subcellular localization, translational efficiency and mRNA half-life. The pre-mRNA splicing can alter mRNP structure and thereby affect downstream mRNA metabolism. The spliceosome stably deposits several proteins on mRNAs as a single complex of approximately 335 kDa. This complex contain several components including the five components that make up the splicing-associated factors are SRm160, DEK and RNPS1, the mRNA-associated shuttling protein Y14 and the mRNA export factor REF (1). Human Daxx (360 kDa) is a protein that functions, in part, as a transcriptional co-repressor through its interaction with a growing number of nuclear, DNA-associated proteins including DEK, a chromatin-associated protein reported to change the topology of DNA in chromatin in vitro and negatively regulate the transcriptional activity.
Discrimination between splice sites and similar, non-splice sequences is essential for correct intron removal and messenger RNA formation in eukaryotes. DEK, a chromatin- and RNA-associated protein mutated or over-expressed in certain cancers, enforces 3’ splice site discrimination by 35 and 65 kDa subunits of U2AF. DEK phosphorylated at serines 19 and 32 associates with U2AF35, facilitates the U2AF35-AG interaction and prevents binding of U2AF65 to pyrimidine tracts not followed by AG. DEK and its phosphorylation are required for intron removal, but not for splicing complex assembly, which indicates that proofreading of early 3’ splice site recognition influences catalytic activation of the spliceosome (3). The DEK proto-oncogene has been associated with human carcinogenesis-either as a fusion with the CAN nucleoporin protein or when transcriptionally upregulated. Mechanisms of intracellular DEK functions, however, have remained relatively unexplored. The chromatin-associated protein DEK was first identified as a fusion protein in patients with a subtype of acute myelogenous leukemia and has been seen associated with a number of other ailments including cancer and autoimmune diseases. The C-terminal region in the Dek (309-375) can reverse the characteristic abnormal DNA-mutagen sensitivity in fibroblast form ataxia-telangiectasia (A-T) patients (2). Dek plays a critical role in cell survival; over-expression of DEK resulted in significant life span extension of primary human keratinocytes. DEK expression protects cancer and primary human cells from apoptotic cell death. Cell death in response to DEK depletion was accompanied by increased protein stability and transcriptional activity of the p53 tumor suppressor and consequent up-regulation of known p53 target genes such as p21CIP and Bax (4).

General readings

Orlic,M., et al., (2006) Genes Chromosomes Cancer 45 (1), 72-82

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DEK antibody

  Rabbit Polyclonal DEK Antibody  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DEK Rabbit Polyclonal antibody. (TA590653) Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DEK (C-term) antibody

DEK Antibody (C-term) (Cat. #AP51234PU-N) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DEK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu F, ICC/IF, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DEK antibody

  Rabbit Polyclonal Anti-DEK Antibody  
WB Suggested Anti-DEK Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

DEK antibody

  Rabbit Polyclonal Anti-DEK Antibody  
WB Suggested Anti-DEK Antibody Titration: 1.25ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

DEK - human gene knockout kit via CRISPR

DEK - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

DEK - human gene knockout kit via CRISPR

DEK - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

DEK - human gene knockout kit via CRISPR

DEK - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DEK (transcript variant 1)

  Recombinant protein of human DEK oncogene (DEK), transcript variant 1  
DEK Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Dek (GFP-tagged) - Mouse DEK oncogene (DNA binding) (Dek), 200 uL, >10^7 TU/mL

Lenti ORF particles, Dek (GFP-tagged) - Mouse DEK oncogene (DNA binding) (Dek), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Dek (Myc-DDK-tagged) - Mouse DEK oncogene (DNA binding) (Dek), 200 uL, >10^7 TU/mL

Lenti ORF particles, Dek (Myc-DDK-tagged) - Mouse DEK oncogene (DNA binding) (Dek), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Dek (GFP-tagged ORF) - Rat DEK oncogene (Dek), 200 uL, >10^7 TU/mL

Lenti ORF particles, Dek (GFP-tagged ORF) - Rat DEK oncogene (Dek), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Dek (Myc-DDK-tagged ORF) - Rat DEK oncogene (Dek), 200 uL, >10^7 TU/mL

Lenti ORF particles, Dek (Myc-DDK-tagged ORF) - Rat DEK oncogene (Dek), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

12 Item(s)

per page
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