DFFA / ICAD antibody

Principal name

DFFA / ICAD antibody

Alternative names for DFFA / ICAD antibody

DFF1, DFF45, DFF-45, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit, Inhibitor of CAD

SwissProt ID

O00273 (Human), O54786 (Mouse)

Gene ID

1676 (DFFA), 13347 (Dffa), 5167606 (IPR000890)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), ELISA (detection) (E(detection)), EPair for Elisa (EPair)

Background of DFFA / ICAD antibody

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

General readings

Ninios,Y.P., et.al., Apoptosis 15 (2), 128-138 (2010).
Banas,T., et.al., Eur. J. Obstet. Gynecol. Reprod. Biol. 146 (1), 87-91 (2009).
Trynka,G., et.al., Gut 58 (8), 1078-1083 (2009).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DFFA / ICAD antibody

Anti-DFFA mouse monoclonal antibody (TA500061) immunofluorescent staining of HeLa cells transiently transfected by  pCMV6-ENTRY DFFA(RC202879) Mouse IgG1 OTI1B10 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DFFA / ICAD antibody

Immunofluorescent staining of A549 cells using anti-DFFA mouse monoclonal antibody (TA500062). Mouse IgG1 OTI5C4 Purified Hu ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

DFFA / ICAD antibody

Anti-DFFA mouse monoclonal antibody (TA500063) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DFFA(RC202879). Mouse IgG1 OTI3F12 Purified Hu, Mky ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DFFA / ICAD (2-21) antibody

Human Pancreas (formalin-fixed, paraffin-embedded) stained with DFFA antibody AP22848PU-N at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

DFFA / ICAD antibody

DFFA MaxPab rabbit polyclonal antibody. Western Blot analysis of DFFA expression in human pancreas. Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

DFFA / ICAD antibody

Immunofluorescence: ICAD Antibody (5C4) [NBP1-47724] - Immunofluorescent staining of A549 cells using anti-DFFA mouse monoclonal antibody. Mouse 5C4 Aff - Purified Hu ICC/IF, WB
0.1 ml / €620.00
  Novus Biologicals Inc.

DFFA / ICAD antibody

Colon carcinoma stained with Anti-DFF-45 antibody Rabbit Purified Hu P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

DFFA / ICAD antibody

Colon carcinoma stained with Anti-DFF-45 antibody Rabbit Purified Hu P, WB
1 ml / €440.00
  Acris Antibodies GmbH

DFFA / ICAD antibody

Colon carcinoma stained with Anti-DFF-45 antibody Rabbit Purified Hu P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

DFFA / ICAD (C-term) antibody

Western blot analysis of DFF45 in HeLa (H), Jurkat (J), A431 (A), and K562 (K) whole cell lysate with DFF45 antibody at 1:1000 dilution. Rabbit IgG Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DFFA / ICAD (N-term) antibody

Western blot analysis of DFF45 in HeLa, (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with DFF45 antibody at 1:1000 dilution. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DFFA / ICAD (N-term) antibody

Western blot analysis of ICAD in mouse lung tissue lysate with ICAD antibody at 1 ug/ml. Rabbit IgG Aff - Purified Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DFFA / ICAD (C-term) antibody

Western blot analysis of ICAD in mouse lung (L), brain (B), liver (L), and kidney tissue lysate with CAD antibody at 1:1000 dilution. Rabbit IgG Aff - Purified Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DFFA / ICAD antibody

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using DFF45 (Cleaved-Asp224) antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

DFFA / ICAD antibody

Predicted cell location: Cytoplasm . Positive control: Human esophagus and ovarian cancer tissue. Recommended dilution: 1/10-100  The image on the left is immunohistochemistry of paraffin-embedded Human esophagus and ovarian cancer tissue using DFFA antibody at dilution 1/5, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

DFFA / ICAD antibody

Predicted cell location: Cytoplasm, Nucleus. Positive control: Human cervical and thyroid cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human cervical and thyroid cancer tissue using DFFA antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

DFFA / ICAD (1-331) antibody

Western blot analysis of DFF45/ICAD expression in Jurkat (1), Raji (2), A431 (3), MRC-5 (4), ZR-75-1 (5), Hep-G2 (6), NIH/3T3 (7), WR19L (8), P19 (9) and PC12 (10) using AM26591AF-N. Mouse IgG1 6B8 Azide Free Hu WB
0.1 mg / €360.00
  Acris Antibodies GmbH

DFFA / ICAD (C-term) antibody

Immunocytochemical staining of HeLa cells with Rabbit anti Human DFF (AP05802PU-N) Rabbit IgG Purified Hu C, ICC/IF, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

DFFA / ICAD antibody

Immunohistochemistry (IHC) analyzes of ICAD antibody (Cat.-No.: AP06798PU-N) in paraffin-embedded human breast carcinoma tissue Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

DFFA / ICAD antibody

Figure 1.: Immunohistochemical staining of  Formalin-Fixed Paraffin-Embedded (FFPE) prostate tissue using antibody AP07742PU-N Rabbit Purified Hu ICC/IF, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

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