DFFB / CAD antibody

Principal name

DFFB / CAD antibody

Alternative names for DFFB / CAD antibody

DFF2, CPAN, DFF-40, DFF40, DNA fragmentation factor subunit beta, DNA fragmentation factor 40 kDa subunit, Caspase-activated deoxyribonuclease, Caspase-activated DNase, Caspase-activated nuclease

SwissProt ID

O54788 (Mouse), O76075 (Human), Q58CZ0 (Bovin), Q99N34 (Rat)

Gene ID

13368 (Dffb), 1677 (DFFB), 790 (CAD)

Available reactivities

Ms (Mouse), Rt (Rat), Hu (Human)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of DFFB / CAD antibody

DFFB is a nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. DFFB degrades naked DNA and induces apoptotic morphology.Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

General / background references:Enari M et al. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 391:43-50 (1998). PubMed PMID: 9422506
Sakahira H et al. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 391:96-9 (1998). PubMed PMID: 9422513
Liu X et al. The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci U S A 95:8461-6 (1998). PubMed PMID: 9671700
Halenbeck R et al. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45. Curr Biol 8:537-40 (1998). PubMed PMID: 9560346

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DFFB / CAD antibody

Western blot analysis of CAD in mouse kidney tissue lysate with CAD antibody at 2 ug/ml. Rabbit IgG Aff - Purified Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DFFB / CAD (C-term) antibody

Western blot analysis of CAD in mouse lung (L) and kidney (K) tissue lysates with CAD antibody at 1:500 dilution. Rabbit IgG Aff - Purified Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DFFB / CAD antibody

Western blot analysis of DFF40 in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with DFF40 antibody (I18) at 1:500 dilution. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DFFB / CAD (N-term) antibody

Western blot analysis of DFF40 in Jurkat (J) and K562 (K) whole cell lysate with DFF40 antibody at 1:500 dilution. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DFFB / CAD antibody

Western blot analysis of DFF40 in Jurkat cell lysate with DFF40 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DFFB / CAD antibody

Predicted cell location: Cytoplasm. Positive control: Human esophagus cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using DFFB antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

DFFB / CAD antibody

WB Suggested Anti-DFFB Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: HT1080 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

DFFB / CAD (205-222) antibody

Immunohistochemical stainning of mouse kidney with Rabbit anti Mouse CAD antibody Cat.-No AP05811PU-N. Rabbit IgG Purified Ms P, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

DFFB / CAD antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Kidney. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

DFFB / CAD Control Peptide

DFFB / CAD
50 µg / €160.00
  Acris Antibodies GmbH

DFFB / CAD Control Peptide

DFFB / CAD
50 µg / €160.00
  Acris Antibodies GmbH

DFFB / CAD Control Peptide

DFFB / CAD
50 µg / €160.00
  Acris Antibodies GmbH

DFFB / CAD Control Peptide

DFFB / CAD
50 µg / €160.00
  Acris Antibodies GmbH

DFFB / CAD Control Peptide

DFFB / CAD
50 µg / €160.00
  Acris Antibodies GmbH

DFFB / CAD (N-term) antibody

Formalin fixed and paraffin embedded prostate carcinoma reacted with DFFB / CAD Antibody (N-term) Cat.-No AP51245PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DFFB / CAD antibody

DFFB polyclonal antibody (A01), Lot # 050928JC01. Western Blot analysis of DFFB expression in Daoy. Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

DFFB / CAD antibody

DFFB MaxPab rabbit polyclonal antibody. Western Blot analysis of DFFB expression in mouse liver. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

DFFB / CAD antibody

DFFB / CAD Mouse IgG2a 4B8 Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

DFFB / CAD antibody

Immunoblot analysis of cell lysates. Lane 1 : 293T cell lysate. Lane 2 : Jurkat T cell lysate. Lane 3 : A-431 cell lysate. Rabbit Hu WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

DFFB / CAD antibody

DFFB / CAD Rabbit Hu, Ms, Rt C, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

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