DFFB / CAD antibody

Principal name

DFFB / CAD antibody

Alternative names for DFFB / CAD antibody

DFF2, CPAN, DFF-40, DFF40, DNA fragmentation factor subunit beta, DNA fragmentation factor 40 kDa subunit, Caspase-activated deoxyribonuclease, Caspase-activated DNase, Caspase-activated nuclease

SwissProt ID

O54788 (Mouse), O76075 (Human), Q58CZ0 (Bovin), Q99N34 (Rat)

Gene ID

1677 (DFFB), 13368 (Dffb), 84359

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C), Flow Cytometry (F)

Background of DFFB / CAD antibody

DFFB is a nuclease that induces D fragmentation and chromatin condensation during apoptosis. DFFB degrades ked D and induces apoptotic morphology.Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal D into nucleosomal units. D fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers D fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both D fragmentation and chromatin condensation during apoptosis. Altertively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications.

General readings

Hanus, J., et al. Apoptosis 13(3):377-382 (2008).
Kalinowska-Herok, M., et al. Acta Biochim. Pol. 55(1):21-26 (2008).
Neimanis, S., et al. J. Biol. Chem. 282(49):35821-35830 (2007).
Hristoskova, S., et al. J. Cell. Physiol. 213(2):490-494 (2007).
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DFFB / CAD antibody

  Rabbit Polyclonal CAD Antibody  
Western blot analysis of CAD in mouse kidney tissue lysate with CAD antibody at 2 ug/ml. Rabbit IgG Aff - Purified Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DFFB / CAD (C-term) antibody

  Rabbit Polyclonal CAD Antibody  
Western blot analysis of CAD in mouse lung (L) and kidney (K) tissue lysates with CAD antibody at 1:500 dilution. Rabbit IgG Aff - Purified Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DFFB / CAD antibody

  Rabbit Polyclonal DFF40 Antibody  
Western blot analysis of DFF40 in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with DFF40 antibody (I18) at 1:500 dilution. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DFFB / CAD (N-term) antibody

  Rabbit Polyclonal DFF40 Antibody  
Western blot analysis of DFF40 in Jurkat (J) and K562 (K) whole cell lysate with DFF40 antibody at 1:500 dilution. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DFFB / CAD antibody

  Rabbit Polyclonal DFF40 Antibody  
Western blot analysis of DFF40 in Jurkat cell lysate with DFF40 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DFFB / CAD antibody

  Anti-DFFB Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human esophagus cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using DFFB antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

DFFB / CAD antibody

  Rabbit Polyclonal Anti-DFFB Antibody  
WB Suggested Anti-DFFB Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: HT1080 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

DFFB / CAD (205-222) antibody

Immunohistochemical stainning of mouse kidney with Rabbit anti Mouse CAD antibody Cat.-No AP05811PU-N. Rabbit IgG Purified Ms P, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

DFFB / CAD antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Kidney. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

DFFB / CAD (N-term) antibody

Formalin fixed and paraffin embedded prostate carcinoma reacted with DFFB / CAD Antibody (N-term) Cat.-No AP51245PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

DFFB - human gene knockout kit via CRISPR

DFFB - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

DFFB - human gene knockout kit via CRISPR

DFFB - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

DFFB - human gene knockout kit via CRISPR

DFFB - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

DFFB - human gene knockout kit via CRISPR

DFFB - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dffb - mouse gene knockout kit via CRISPR

Dffb - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Dffb - mouse gene knockout kit via CRISPR

Dffb - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dffb - mouse gene knockout kit via CRISPR

Dffb - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dffb - mouse gene knockout kit via CRISPR

Dffb - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Dffb (GFP-tagged) - Mouse DNA fragmentation factor, beta subunit (Dffb), 200 uL, >10^7 TU/mL

Lenti ORF particles, Dffb (GFP-tagged) - Mouse DNA fragmentation factor, beta subunit (Dffb), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Dffb (Myc-DDK-tagged) - Mouse DNA fragmentation factor, beta subunit (Dffb), 200 uL, >10^7 TU/mL

Lenti ORF particles, Dffb (Myc-DDK-tagged) - Mouse DNA fragmentation factor, beta subunit (Dffb), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

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