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DGAT1 antibody

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Principal name

DGAT1 antibody

Alternative names for DGAT1 antibody

DGAT-1, AGRP1, DGAT, Diglyceride acyltransferase, Diacylglycerol O-acyltransferase 1, CAT-related gene product 1

SwissProt ID

O75907 (Human), Q8MK44 (Bovin), Q9ERM3 (Rat), Q9GMF1 (Chlae), Q9Z2A7 (Mouse)

Gene ID

8694 (DGAT1), 727765, 13350 (Dgat1)

Ncbi ID

NP_036211.2, NP_036211, 10090, NM_012079

Available reactivities

Hu (Human), Rt (Rat), Bov (Bovine), Gt (Goat), Mky (Monkey), Ms (Mouse), Sh (Sheep), Ze (Zebrafish), Can (Canine), Por (Porcine), Prim (Primate), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP)

Background of DGAT1 antibody

Glucose and insulin are anabolic signals which upregulate the transcriptions of a series of lipogenic enzymes to convert excess carbohydrate into triglycerides for efficient energy storage. DGAT1 is a microsomal enzyme that assists in the synthesis of fatty acids into triglycerides. DGAT catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol (DAG) and fatty acyl CoA as substrates. DGAT plays a fundamental role in the metabolism of cellular diacylglycerol and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism such as intestinal fat absorption, lipoprotein assembly, adipose tissue formation, and lactation. DGAT1 is involved in fat absorption in the intestine and in basal level triglyceride synthesis in adipose tissue, where it is more highly expressed.

General readings

Bagnato C, Igal RA. Overexpression of diacylglycerol acyltransferase-1 reduces phospholipid synthesis, proliferation, and invasiveness in simian virus 40-transformed human lung fibroblasts. J Biol Chem. 2003 Dec 26;278(52):52203-11. Epub 2003 Oct 13. PMID: 14557275

NB100-57086 - DGAT1
Western Blot: DGAT1 Antibody [NB100-57086] - NB100-57086 (0.5ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NB110-41487 - DGAT1
Western Blot: DGAT1 Antibody [NB110-41487] - Detection of DGAT1 in HepG2 lysate.
PAB7180 - DGAT1
DGAT1 polyclonal antibody ( Cat # PAB7180 ) ( 0.5 µg/ml ) staining of human duodenum lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
AP16667PU-N - DGAT1
AP16667PU-N DGAT1 antibody staining of Rat Duodenum lysate at 0.3 µg/ml (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
PAB12449 - DGAT1
Western blot analysis of DGAT1 in HepG2 lysate using DGAT1 polyclonal antibody ( Cat # PAB12449 ).
PAB8674 - DGAT1
Western blot analysis of Dgat1 in lysate from Jurkat cells ( Lane 1, 2 ) , NIH/3T3 cells ( Lane 3 ) and rat kidney tissue lysate ( Lane 4 ) .
AP32857PU-N - DGAT1
AP32857PU-N (0.5µg/ml) staining of Mouse Duodenum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
GTX48577 - DGAT1
Western Blot: DGAT1 Antibody [GTX48577] - Detection of DGAT1 in HepG2 lysate.
GTX48577 - DGAT1
Immunocytochemistry/Immunofluorescence: DGAT1 Antibody [GTX48577] - DGAT1 antibody was tested in Hek293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
GTX89164 - DGAT1
GTX89164 (0.3µg/ml) staining of Rat Duodenum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
GTX89164 - DGAT1
GTX89164 (0.5µg/ml) staining of human duodenum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
11561-1-AP - DGAT1
Immunohistochemical of paraffin-embedded human pancreas cancer using 11561-1-AP(DGAT1 antibody) at dilution of 1:50 (under 10x lens)
11561-1-AP - DGAT1
Immunohistochemistry of paraffin-embedded human hepatocirrhosis tissue slide using 11561-1-AP( DGAT1 Antibody) at dilution of 1:50 (under 10x lens)
11561-1-AP - DGAT1
Immunohistochemistry of paraffin-embedded human hepatocirrhosis tissue slide using 11561-1-AP( DGAT1 Antibody) at dilution of 1:50 (under 40x lens)
11561-1-AP - DGAT1
Mouse heart tissue were subjected to SDS PAGE followed by western blot with 11561-1-AP( DGAT1 Antibody) at dilution of 1:1000
11561-1-AP - DGAT1
IP Result of anti-DGAT1 (IP:11561-1-AP, 3ug; Detection:11561-1-AP 1:300) with mouse heart tissue lysate 4000ug.
bs-2332R - DGAT1
Antigen: bs-2332P, 0.2ug/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;TMB staining;Read the data in MicroplateReader by 450
bs-2332R - DGAT1
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti DGAT1/Diglyceride acyltransferase Polyclonal Antibody, Unconjugated (bs-2332R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
bs-2332R - DGAT1
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti DGAT1/Diglyceride acyltransferase Polyclonal Antibody, Unconjugated (bs-2332R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

14 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DGAT1 (67-79) antibody
AP32857PU-N (0.5µg/ml) staining of Mouse Duodenum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH
 

Acris recommends this product for Western blotting.

DGAT1 antibody
TA303180 (0.5µg/ml) staining of human duodenum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DGAT1 antibody
Western Blot: DGAT1 Antibody - Detection of DGAT1 in HepG2 lysate. Rabbit Purified Bov, Gt, Hu, Ms, Prim, Rt, Sh, Ze WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DGAT1 antibody
Western blot analysis of DGAT-1 in lysates from Jurkat cells (Lane 1, 2), 3T3 cells (Lane 3), and rat kidney tissue lysate (Lane 4). Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DGAT1 antibody
Host: Rabbit; Target Name: Dgat1; Sample Tissue: Mouse Heart lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

DGAT1 antibody
DGAT1 polyclonal antibody ( Cat # PAB7180 ) ( 0.5 µg/ml ) staining of human duodenum lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu, Rt E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

DGAT1 antibody
Western blot analysis of Dgat1 in lysate from Jurkat cells ( Lane 1, 2 ) , NIH/3T3 cells ( Lane 3 ) and rat kidney tissue lysate ( Lane 4 ) . Rabbit Hu, Ms, Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

DGAT1 antibody
Western blot analysis of DGAT1 in HepG2 lysate using DGAT1 polyclonal antibody ( Cat # PAB12449 ). Rabbit IgG Bov, Gt, Hu, Ms, Rt, Sh WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

DGAT1 antibody
Western Blot: DGAT1 Antibody [NB100-57086] - NB100-57086 (0.5ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu, Rt E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

DGAT1 antibody
DGAT1 Rabbit Biotin Bov, Hu, Ms, Rt, Ze WB
0.1 ml / €360.00
  Novus Biologicals Inc.

DGAT1 antibody
DGAT1 Rabbit DyLight488 Bov, Hu, Ms, Rt, Ze WB
0.1 ml / €390.00
  Novus Biologicals Inc.

DGAT1 antibody
DGAT1 Rabbit HRP Bov, Hu, Ms, Rt, Ze WB
0.1 ml / €390.00
  Novus Biologicals Inc.

DGAT1 antibody
DGAT1 Rabbit Aff - Purified Bov, Gt, Hu, Mky, Ms, Rt, Sh, Ze ICC/IF, WB
25 µl / €200.00
  Novus Biologicals Inc.

Lysates (Overexpressed)

Catalog No.    

DGAT1 overexpression lysate
DGAT1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

14 Item(s)

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Product Type
  1. Lysate (1)
  2. Primary Antibody (13)
Clonality
  1. Polyclonal (13)
Target
  1. DGAT1 (13)
Property
  1. 67-79 (1)
Reactivity
  1. Human (Hu) (13)
  2. Rat (Rt) (13)
  3. Mouse (Ms) (11)
  4. Bovine (Bov) (8)
  5. Zebrafish (Ze) (6)
  6. Goat (Gt) (4)
  7. Sheep (Sh) (4)
  8. Canine (Can) (3)
  9. Porcine (Por) (2)
  10. Equine (Eq) (1)
  11. Guinea Pig (GP) (1)
  12. Monkey (Mky) (1)
  13. Prim (1)
  14. Rabbit (Rb) (1)
Applications
  1. Western blot / Immunoblot (WB) (13)
  2. Enzyme Immunoassay (E) (4)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  4. Paraffin Sections (P) (1)
Presentation
  1. Aff - Purified (4)
  2. Purified (3)
  3. Biotin (1)
  4. DyLight488 (1)
  5. HRP (1)
Host
  1. Rabbit (9)
  2. Goat (4)
Images
  1. 1 image (9)
Images: Applications
  1. Western blot / Immunoblot (WB) (9)
Manufacturer
  1. Acris - OriGene (6)
  2. Novus Biologicals Inc. (5)
  3. Abnova Taiwan Corp. (3)
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