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DGAT1 antibody

Principal name

DGAT1 antibody

Alternative names for DGAT1 antibody

DGAT-1, AGRP1, DGAT, Diglyceride acyltransferase, Diacylglycerol O-acyltransferase 1, CAT-related gene product 1

SwissProt ID

O75907 (Human), Q8MK44 (Bovin), Q9ERM3 (Rat), Q9GMF1 (Chlae), Q9Z2A7 (Mouse)

Gene ID

8694 (DGAT1), 13350 (Dgat1), 84497

Ncbi ID

NP_036211.2, NP_036211, 10090, NM_012079, NM_010046, NM_053437, NP_034176.1

Available reactivities

Hu (Human), Rt (Rat), Bov (Bovine), Gt (Goat), Ms (Mouse), Sh (Sheep), Can (Canine), Por (Porcine), Prim (Primate), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of DGAT1 antibody

Glucose and insulin are anabolic signals which upregulate the transcriptions of a series of lipogenic enzymes to convert excess carbohydrate into triglycerides for efficient energy storage. DGAT1 is a microsomal enzyme that assists in the synthesis of fatty acids into triglycerides. DGAT catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol (DAG) and fatty acyl CoA as substrates. DGAT plays a fundamental role in the metabolism of cellular diacylglycerol and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism such as intestinal fat absorption, lipoprotein assembly, adipose tissue formation, and lactation. DGAT1 is involved in fat absorption in the intestine and in basal level triglyceride synthesis in adipose tissue, where it is more highly expressed.

DGAT1 polyclonal antibody ( Cat # PAB7180 ) ( 0.5 µg/ml ) staining of human duodenum lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.

PAB7180

Western blot analysis of DGAT1 in HepG2 lysate using DGAT1 polyclonal antibody ( Cat # PAB12449 ).

PAB12449

Western blot analysis of Dgat1 in lysate from Jurkat cells ( Lane 1, 2 ) , NIH/3T3 cells ( Lane 3 ) and rat kidney tissue lysate ( Lane 4 ) .

PAB8674

AP32857PU-N (0.5µg/ml) staining of Mouse Duodenum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

AP32857PU-N

TA303180 (0.5µg/ml) staining of human duodenum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA303180

Western Blot: DGAT1 Antibody - Detection of DGAT1 in HepG2 lysate.

TA309624

Western blot analysis of DGAT-1 in lysates from Jurkat cells (Lane 1, 2), 3T3 cells (Lane 3), and rat kidney tissue lysate (Lane 4).

TA318979

Host: Rabbit; Target Name: Dgat1; Sample Tissue: Mouse Heart lysates; Antibody Dilution: 1.0 ug/ml

TA343309

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
AP32857PU-N	DGAT1 (67-79) antibody
	Goat		Aff - Purified	Bov, Can, Ms, Por, Rt	E, WB	0.1 mg / €350.00
						Acris Antibodies GmbH
+1 additional image	Acris recommends this product for Western blotting.
TA303180	DGAT1 antibody
	Goat Polyclonal Antibody against DGAT1
	Goat		Aff - Purified	Can, Hu, Ms, Rt	E, P, WB	0.1 mg / €325.00
						OriGene Technologies, Inc.
TA309624	DGAT1 antibody
	Rabbit Polyclonal DGAT1 Antibody
	Rabbit		Purified	Bov, Gt, Hu, Ms, Prim, Rt, Sh, Ze	WB	0.1 ml / €325.00
						OriGene Technologies, Inc.
TA318979	DGAT1 antibody
	Rabbit polyclonal anti-DGAT1 antibody
	Rabbit		Purified	Hu, Ms, Rt	WB	0.1 mg / €325.00
						OriGene Technologies, Inc.
TA343309	DGAT1 antibody
	Rabbit Polyclonal Anti-DGAT1 Antibody
	Rabbit	IgG	Purified	Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Sh, Ze	WB	50 µg / €325.00
						OriGene Technologies, Inc.
TA351137	DGAT1 antibody
	Rabbit Polyclonal Anti-DGAT1 Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / €325.00
						OriGene Technologies, Inc.

CRISPR

Catalog No.	Species	Donor Vector
KN304520LP	Dgat1 - mouse gene knockout kit via CRISPR
	Mouse	Luciferase-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN304520RB	Dgat1 - mouse gene knockout kit via CRISPR
	Mouse	RFP-BSD	1 kit / €1,290.00
			OriGene Technologies, Inc.

Lysates

Catalog No.
LY402142	DGAT1 overexpression lysate
		0.1 mg / €280.00
		OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.
RC220595L2V	Lenti ORF particles, DGAT1 (mGFP-tagged)-Human diacylglycerol O-acyltransferase 1 (DGAT1), 200 uL, >10^7 TU/mL
		200 µl / €1,050.00
		OriGene Technologies, Inc.

RC220595L1V	Lenti ORF particles, DGAT1 (Myc-DDK-tagged)-Human diacylglycerol O-acyltransferase 1 (DGAT1), 200 uL, >10^7 TU/mL
		200 µl / €940.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR225809L2V	Lenti ORF particles, Dgat1 (GFP-tagged) - Mouse diacylglycerol O-acyltransferase 1 (Dgat1), 200 uL, >10^7 TU/mL
		200 µl / €1,120.00
		OriGene Technologies, Inc.

MR225809L1V	Lenti ORF particles, Dgat1 (Myc-DDK-tagged) - Mouse diacylglycerol O-acyltransferase 1 (Dgat1), 200 uL, >10^7 TU/mL
		200 µl / €1,000.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR211358L2V	Lenti ORF particles, Dgat1 (GFP-tagged ORF) - Rat diacylglycerol O-acyltransferase homolog 1 (mouse) (Dgat1), 200 uL, >10^7 TU/mL
		200 µl / €1,120.00
		OriGene Technologies, Inc.

RR211358L1V	Lenti ORF particles, Dgat1 (Myc-DDK-tagged ORF) - Rat diacylglycerol O-acyltransferase homolog 1 (mouse) (Dgat1), 200 uL, >10^7 TU/mL
		200 µl / €999.00
		OriGene Technologies, Inc.

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