DGCR8 antibody

Principal name

DGCR8 antibody

Alternative names for DGCR8 antibody

C22orf12, DGCRK6, LP4941, DiGeorge syndrome critical region 8, Microprocessor complex subunit DGCR8

SwissProt ID

Q8WYQ5 (Human), Q9EQM6 (Mouse), Q9H6T7 (Human)

Gene ID

54487 (DGCR8)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of DGCR8 antibody

DGCR8 (UniProtKB/Swiss-Prot entry Q8WYQ5) is a component of the microprocessor complex that acts as a R- and heme-binding protein and is involved in the initial step of microR (miR) biogenesis. This microprocessor complex that is required to process primary miR transcripts (pri-miRs) to release precursor miR (pre-miR) in the nucleus. Within the microprocessor complex, DGCR8 functions as a molecular anchor necessary for the recognition of pri-miR at dsR-ssR junction and directs DROSHA to cleave 11 bp away form the junction. This releases hairpin-shaped pre-miRs that are subsequently cut by the cytoplasmic DICER to generate mature miRs. The heme-bound DGCR8 dimer binds pri-miRs as a cooperative trimer (of dimers) and is active in triggering pri-miR cleavage, whereas the heme-free DGCR8 monomer binds pri-miRs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miR are required for its binding. DGCR8 is involved in the silencing of embryonic stem cells self-renewal.

General readings

Yeom KH, Lee Y, Han J, Suh MR, Kim VN. Characterization of DGCR8/Pasha, the essential cofactor for Drosha in primary miRNA processing.Nucleic Acids Res. 2006;34(16):4622-9. Epub 2006 Sep 8. PMID: 16963499

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DGCR8 antibody

Western blot analysis of rat and human testis lysates with anti-DGCR8 antibody (1:500). Rabbit Purified Hu, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DGCR8 antibody

WB Suggested Anti-DGCR8 Antibody; Positive Control: Lane 1: Cytoplasmic fraction of mouse back skin lysate Lane 2: Nuclear fraction of mouse back skin lysate Lane 3: Cytoplasmic fraction of mouse back skin lysate Lane 4: Nuclear fraction of mouse back ski Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

DGCR8 antibody

Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against mouse DGCR8. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:8,100. Rabbit IgG Purified Ms E
0.1 ml / €325.00
  OriGene Technologies, Inc.

DGCR8 antibody

DGCR8 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

DGCR8 antibody

Immunofluorescence of purified MaxPab antibody to DGCR8 on HeLa cell. [antibody concentration 20 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

DGCR8 antibody

DGCR8 Rabbit Serum Hu, Ms E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

DGCR8 antibody

DGCR8 MaxPab rabbit polyclonal antibody. Western Blot analysis of DGCR8 expression in mouse lung. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

DGCR8 antibody

DGCR8 Mouse IgG2b 4G3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DGCR8 antibody

Human Liver; Liver; DGCR8 antibody - N-terminal region (ARP40984_P050) in Human Liver cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

DGCR8 antibody

DGCR8 Goat IgG Aff - Purified Ms E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

DGCR8 antibody

WB and IP: DGCR8 Antibody [NBP1-30115] - Whole cell lysate from HeLa and 293T cells. DGCR8 was also IPed by rabbit anti-DGCR8 antibody NBP1-30116. For blotting immunoprecipitated DGCR8, the ReliaBLOT®  Reagents and Procedures were used. Rabbit Aff - Purified Hu ICC/IF, IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

DGCR8 antibody

WB of IP: STK11IP  Antibody [NBP1-30116] - Whole cell lysate from HeLa cells. NBP1-30116 used for IP at 3 mcg/mg lysate. STK11IP was also IPed by rabbit STK11IP antibody NBP1-30115. For blotting IPed STK11IP , NBP1-30115 was used at 1 mcg/ml. Rabbit Aff - Purified Hu ICC/IF, IP, P
0.1 ml / €440.00
  Novus Biologicals Inc.

DGCR8 antibody

Western Blot: DGCR8 Antibody [NBP1-57261] - This Anti-DGCR8 antibody was used in Western Blot of Hela tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DGCR8

DGCR8 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

DGCR8

DGCR8 Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

DGCR8

DGCR8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

DGCR8

DGCR8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

DGCR8 (Human) IP-WB Antibody Pair

DGCR8 (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

DGCR8 293T Cell Transient Overexpression Lysate(Denatured)

DGCR8 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

DGCR8 overexpression lysate

DGCR8 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

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