DGCR8 antibody

Principal name

DGCR8 antibody

Alternative names for DGCR8 antibody

C22orf12, DGCRK6, LP4941, DiGeorge syndrome critical region 8, Microprocessor complex subunit DGCR8

SwissProt ID

Q8WYQ5 (Human), Q9EQM6 (Mouse), Q9H6T7 (Human)

Gene ID

54487 (DGCR8), 94223 (Dgcr8)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Rt (Rat), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)

Background of DGCR8 antibody

DGCR8 (UniProtKB/Swiss-Prot entry Q8WYQ5) is a component of the microprocessor complex that acts as a R- and heme-binding protein and is involved in the initial step of microR (miR) biogenesis. This microprocessor complex that is required to process primary miR transcripts (pri-miRs) to release precursor miR (pre-miR) in the nucleus. Within the microprocessor complex, DGCR8 functions as a molecular anchor necessary for the recognition of pri-miR at dsR-ssR junction and directs DROSHA to cleave 11 bp away form the junction. This releases hairpin-shaped pre-miRs that are subsequently cut by the cytoplasmic DICER to generate mature miRs. The heme-bound DGCR8 dimer binds pri-miRs as a cooperative trimer (of dimers) and is active in triggering pri-miR cleavage, whereas the heme-free DGCR8 monomer binds pri-miRs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miR are required for its binding. DGCR8 is involved in the silencing of embryonic stem cells self-renewal.

General readings

Shiohama,A., (2007) Exp. Cell Res. 313 (20), 4196-4207

19 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DGCR8 antibody

  Rabbit anti-DGCR8 polyclonal antibody  
Western blot analysis of rat and human testis lysates with anti-DGCR8 antibody (1:500). Rabbit Purified Hu, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DGCR8 antibody

  Rabbit Polyclonal Anti-DGCR8 Antibody  
WB Suggested Anti-DGCR8 Antibody; Positive Control: Lane 1: Cytoplasmic fraction of mouse back skin lysate Lane 2: Nuclear fraction of mouse back skin lysate Lane 3: Cytoplasmic fraction of mouse back skin lysate Lane 4: Nuclear fraction of mouse back ski Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

DGCR8 antibody

  Rabbit Polyclonal DGCR8 Antibody  
Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against mouse DGCR8. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:8,100. Rabbit IgG Purified Ms E
0.1 ml / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

DGCR8 - human gene knockout kit via CRISPR

DGCR8 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

DGCR8 - human gene knockout kit via CRISPR

DGCR8 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

DGCR8 - human gene knockout kit via CRISPR

DGCR8 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

DGCR8 - human gene knockout kit via CRISPR

DGCR8 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dgcr8 - mouse gene knockout kit via CRISPR

Dgcr8 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Dgcr8 - mouse gene knockout kit via CRISPR

Dgcr8 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dgcr8 - mouse gene knockout kit via CRISPR

Dgcr8 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dgcr8 - mouse gene knockout kit via CRISPR

Dgcr8 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DGCR8

  Purified recombinant protein of Homo sapiens DiGeorge syndrome critical region gene 8 (DGCR8)  
DGCR8 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

DGCR8 overexpression lysate

DGCR8 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, DGCR8 (mGFP-tagged) - Human DiGeorge syndrome critical region gene 8 (DGCR8), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, DGCR8 (mGFP-tagged) - Human DiGeorge syndrome critical region gene 8 (DGCR8), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,680.00
  OriGene Technologies, Inc.

Lenti ORF particles, DGCR8 (mGFP-tagged)-Human DiGeorge syndrome critical region gene 8 (DGCR8), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, DGCR8 (mGFP-tagged)-Human DiGeorge syndrome critical region gene 8 (DGCR8), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, DGCR8 (Myc-DDK tagged) - Human DiGeorge syndrome critical region gene 8 (DGCR8), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, DGCR8 (Myc-DDK tagged) - Human DiGeorge syndrome critical region gene 8 (DGCR8), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €1,510.00
  OriGene Technologies, Inc.

Lenti ORF particles, DGCR8 (Myc-DDK-tagged)-Human DiGeorge syndrome critical region gene 8 (DGCR8), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, DGCR8 (Myc-DDK-tagged)-Human DiGeorge syndrome critical region gene 8 (DGCR8), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Dgcr8 (GFP-tagged) - Mouse DiGeorge syndrome critical region gene 8 (Dgcr8), 200 uL, >10^7 TU/mL

Lenti ORF particles, Dgcr8 (GFP-tagged) - Mouse DiGeorge syndrome critical region gene 8 (Dgcr8), 200 uL, >10^7 TU/mL  
200 µl / €1,480.00
  OriGene Technologies, Inc.

Lenti ORF particles, Dgcr8 (Myc-DDK-tagged) - Mouse DiGeorge syndrome critical region gene 8 (Dgcr8), 200 uL, >10^7 TU/mL

Lenti ORF particles, Dgcr8 (Myc-DDK-tagged) - Mouse DiGeorge syndrome critical region gene 8 (Dgcr8), 200 uL, >10^7 TU/mL  
200 µl / €1,330.00
  OriGene Technologies, Inc.

19 Item(s)

per page
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