DHFR / DHFRP1 antibody

Principal name

DHFR / DHFRP1 antibody

Alternative names for DHFR / DHFRP1 antibody

Dihydrofolate reductase

SwissProt ID

P00374 (Human), P00375 (Mouse), P00376 (Bovin), P00377 (Pig), P00378 (Chick), Q920D2 (Rat)

Gene ID

1719 (DHFR), 13361 (Dhfr), 24312

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E)

Background of DHFR / DHFRP1 antibody

Kit Component:
- KN200089G1, DHFR gRNA vector 1 in pCas-Guide vector
- KN200089G2, DHFR gRNA vector 2 in pCas-Guide vector
- KN200089D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Chen MJ., et al. (1984) J Biol Chem. 259(6): 3933-3943. Cody V., et al. (2009) Biochemistry. 48(8): 1702-1711.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DHFR / DHFRP1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-DHFR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500517, Dilution 1:50) Mouse IgG2b OTI3A11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DHFR / DHFRP1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-DHFRmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500543, Dilution 1:50) Mouse IgG1 OTI6G7 Purified Can, Hu, Mky ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

DHFR / DHFRP1 antibody

Immunofluorescence of monoclonal antibody to DHFR on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 2B10 Purified Hu, Rt E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DHFR / DHFRP1 antibody

AP15522PU DHFR antibody staining of Human Breast Carcinoma. Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

DHFR / DHFRP1 antibody

AP15522PU DHFR antibody staining of Human Breast Carcinoma. Rabbit Purified Hu P
1 ml / €530.00
  Acris Antibodies GmbH

DHFR / DHFRP1 antibody

AP15522PU DHFR antibody staining of Human Breast Carcinoma. Rabbit Purified Hu P
0.1 ml / €200.00
  Acris Antibodies GmbH

DHFR / DHFRP1 antibody

Predicted band size: 21 kDa . Positive control: Hela and K562 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: K562 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DHFR / DHFRP1 antibody

Western blot analysis of extracts of various cell lines, using DHFR antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

DHFR / DHFRP1 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DHFR antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : HeLa cell lysate 
Lane 2. : Jurkat cell lysate
Lane 3 : 293T cell lysate Mouse IgG1 AT5B2 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

DHFR / DHFRP1 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DHFR antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : HeLa cell lysate 
Lane 2. : Jurkat cell lysate
Lane 3 : 293T cell lysate Mouse IgG1 AT5B2 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

DHFR / DHFRP1 (C-term) antibody

Western blot analysis of DHFR antibody (C-term) (AP17565PU-N) in mouse liver tissue lysates (35ug/lane). KLF4 (arrow) was detected using the purified Pab. Rabbit Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DHFR / DHFRP1 antibody

Immunofluorescence of purified MaxPab antibody to DHFR on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

DHFR / DHFRP1 antibody

Western Blot analysis of DHFR expression in transfected 293T cell line (H00001719-T01) by DHFR MaxPab polyclonal antibody.

Lane 1: DHFR transfected lysate(21.50 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DHFR / DHFRP1

DHFR / DHFRP1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

DHFR / DHFRP1 (1-187, His-tag)

Recombinant human Dihydrofolate reductase (DHFR), 1-187 aa,  His-tagged: 15% SDS-PAGE (3 µg) Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €540.00
  Acris Antibodies GmbH

DHFR / DHFRP1 (1-187, His-tag)

Recombinant human Dihydrofolate reductase (DHFR), 1-187 aa,  His-tagged: 15% SDS-PAGE (3 µg) Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €230.00
  Acris Antibodies GmbH

DHFR / DHFRP1 (1-187, His-tag)

DHFR / DHFRP1 Mouse Purified > 95 % E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

DHFR / DHFRP1 (1-187, His-tag)

DHFR / DHFRP1 Mouse Purified > 95 % E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

DHFR / DHFRP1

DHFR / DHFRP1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

DHFR / DHFRP1

DHFR / DHFRP1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

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