DIABLO / SMAC antibody

Principal name

DIABLO / SMAC antibody

Alternative names for DIABLO / SMAC antibody

Diablo homolog mitochondrial, Second mitochondria-derived activator of caspase, Direct IAP-binding protein with low pI

SwissProt ID

Q9JIQ3 (Mouse), Q9NR28 (Human)

Gene ID

56616 (DIABLO), 66593 (Diablo)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Mky (Monkey)

Available hosts

Rabbit, Mouse, Rat

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), ELISA (detection) (E(detection))

Background of DIABLO / SMAC antibody

Apoptosis is a conserved cell suicide program essential for the development and homeostasis of multicellular organisms. Abnormal inhibition of apoptosis is a hallmark of cancer and autoimmune diseases, whereas excessive cell death is found in neurodegenerative disorders such as Alzheimers disease. Executioners of the apoptotic program are cysteine proteases termed caspases that exist as inactive zymogens in living cells and are activated during apoptosis. Active caspases cleave key intracellular protein substrates, resulting in the characteristic morphological changes associated with apoptosis. The release of cytochrome c from the mitochondria triggers the oligomerization of Apaf-1 in an ATP/dATP-dependent manner and induces the autoactivation of caspase-9. Active caspase-9 in turn activates downstream effector caspases including caspase -3, -6 and -7.
Given the potentially irreversible cascade triggered by the activation of initiator caspases, it is crucial that activation of caspases be tightly regulated. A number of cellular proteins have been shown to modulate caspase activation and activity. These include members of the Inhibitor of the Apoptosis Protein (IAP) family, such as XIAP, c-IAP-1 and c-IAP-2, which block cell death by inhibiting the activity of the effector caspases -3 and -7 and the initiator caspase-9. Smac/DIABLO (Second Mitochondria-derived Activator of Caspase) is a mitochondrial protein of 27 kDa that is released from mitochondria during apoptosis. Smac/DIABLO promotes caspase activation through binding and neutralizing IAPs.

General readings

McNeish. I.A., et al. (2003) Exp Cell Res 286(2): 186–198.
Yu. J., et al. (2007) Oncogene 26(29): 4189–4198.
Vucic. D., et al. (2002) J Biol Chem 277(14): 12275–12279.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DIABLO / SMAC antibody

Western blot analysis of extracts of various cell lines, using DIABLO antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

DIABLO / SMAC antibody

Immunohistochemistry: AM20170PU-N DIABLO antibody staining of Formalin-Fixed, Paraffin-Embedded Human Tonsil followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG2b 79-1-83 Aff - Purified Hu, Mky P, WB
50 µg / €470.00
  Acris Antibodies GmbH

DIABLO / SMAC antibody

Western blot analysis of Smac in human heart tissue lysate in the absence (A) or presence (B) of blocking peptide (2409P) with Smac antibody at 1 ug /ml. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DIABLO / SMAC (C-term) antibody

Western blot analysis of Smac in mouse heart tissue lysate in the absence (A) or presence (B) of blocking peptide and in rat heart tissue lysate with Smac antibody at 1 ug /ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DIABLO / SMAC antibody

Western Blot: Smac Antibody - Western blot analysis of Smac on HeLa whole cell extract lysates. Mouse IgG3 78-1-118 Purified Hu, Ms, Rt IP, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

DIABLO / SMAC (56-239) antibody

WB using anti-Smac detects a 26 kDa band when 1 µg of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 µg of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 µg each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 µM etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. The primary antibody was used at 1:1000. HRP Goat-a-Rabbit antibody was 1:1000. Rabbit IgG Serum Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DIABLO / SMAC antibody

Predicted band size: 27 kDa . Positive control: Lncap, SKOV3, MCF7 and 293T cell lysate. Recommended dilution: 1/1000-5000. (Gel: 12%SDS-PAGE Lane1: Lncap cell lysate Lane2: SKOV3 cell lysate Lane3: MCF7 cell lysate Lane4: 293T cell lysate Lysates: 40 ug per lane Primary antibody: 1/1000 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 second) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DIABLO / SMAC antibody

Predicted band size: 27 kDa . Positive control: Lncap, SKOV3, MCF7 and 293T cell lysate. Recommended dilution: 1/1000-5000. (Gel: 12%SDS-PAGE Lane1: Lncapcell lysate Lane2: SKOV3 cell lysate Lane3: MCF7 cell lysate Lane4: 293T cell lysate Lysates: 40 ug per lane Primary antibody: 1/800 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 second) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DIABLO / SMAC antibody

WB Suggested Anti-DIABLO Antibody Titration: 0.3125 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DIABLO / SMAC antibody

Western Blot: Smac Antibody [TA336333] - Analysis of Smac (DIABLO) in HeLa lysate using Smac antibody at 2 ug/ml. Rabbit Purified Hu ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DIABLO / SMAC antibody

DIABLO / SMAC Mouse IgG2b 2D12 Purified Hu E, WB
0.1 mg / €410.00
  Acris Antibodies GmbH

DIABLO / SMAC antibody

The cell lysate of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SMAC antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2a AT19F2 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

DIABLO / SMAC antibody

The cell lysate of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SMAC antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2a AT19F2 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

DIABLO / SMAC antibody

Testis: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

DIABLO / SMAC Control Peptide

DIABLO / SMAC
50 µg / €160.00
  Acris Antibodies GmbH

DIABLO / SMAC (225-239) antibody

Smac/DIABLO – SP1308 staining of human ovary tissue with anti-Smac/DIABLO (CT) at 5
µg /ml. Formalin fixed paraffin processed tissue. Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

DIABLO / SMAC (222-237) antibody

Imunohistochemical staining of mouse spleen with DIABLO / SMAC antibody Cat.-No SP1309. Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

DIABLO / SMAC antibody

DIABLO MaxPab polyclonal antibody. Western Blot analysis of DIABLO expression in human kidney. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

DIABLO / SMAC antibody

DIABLO / SMAC Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

DIABLO / SMAC antibody

Western Blot analysis of DIABLO expression in transfected 293T cell line (H00056616-T01) by DIABLO MaxPab polyclonal antibody.

Lane 1: DIABLO transfected lysate(27.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

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