DIAPH1 antibody

Principal name

DIAPH1 antibody

Alternative names for DIAPH1 antibody

DIAP1, Diaphanous homolog 1, Diaphanous-1, DRF1, Diaphanous-related formin-1

SwissProt ID

O08808 (Mouse), O60610 (Human)

Gene ID

1729 (DIAPH1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection))

Background of DIAPH1 antibody

Acts in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DIAPH1 antibody

Sample (30 ug of whole cell lysate). A: H1299. B: Raji. 7.5% SDS PAGE. TA308571 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DIAPH1 antibody

(0.3ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DIAPH1 antibody

Western blot analysis of extracts of various cell lines, using DIAPH1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

DIAPH1 antibody

DIAPH1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

DIAPH1 antibody

Western Blot analysis of DIAPH1 expression in transfected 293T cell line (H00001729-T01) by DIAPH1 MaxPab polyclonal antibody.

Lane 1: DIAPH1 transfected lysate(44.44 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

DIAPH1 antibody

DIAPH1 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

DIAPH1 antibody

Western Blot analysis of DIAPH1 expression in transfected 293T cell line (H00001729-T02) by DIAPH1 MaxPab polyclonal antibody.

Lane 1: DIAPH1 transfected lysate(44.44 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

DIAPH1 antibody

Detection limit for recombinant GST tagged DIAPH1 is approximately 0.1ng/ml as a capture antibody. Mouse IgG2b 5A8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DIAPH1 antibody

DIAPH1 Mouse IgG2b 1A8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DIAPH1 antibody

Western Blot: Diaphanous 1 Antibody [NB600-230] - Detection of Human Diaphanous 1 by Western blot.  Samples: Whole cell lysate (10 or 50 mcg/lane) from untreated or siRNA treated 293T cells.  NB600-230 used at 0.1 mcg and 0.04 mcg/ml. Rabbit Purified Hu WB
0.1 ml / €440.00
  Novus Biologicals Inc.

DIAPH1 antibody

Immunohistochemistry-Paraffin: DIAPH1 Antibody [NB600-231] - Please see notes section Rabbit Purified Hu ICC/IF, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

DIAPH1 antibody

DIAPH1 Goat Aff - Purified Ms E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DIAPH1 (transcript variant 2)

DIAPH1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

DIAPH1 (transcript variant 1)

DIAPH1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

DIAPH1

DIAPH1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

DIAPH1

DIAPH1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

DIAPH1 293T Cell Transient Overexpression Lysate(Denatured)

DIAPH1 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

DIAPH1 overexpression lysate

DIAPH1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

18 Item(s)

per page
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