DIAPH2 antibody

Principal name

DIAPH2 antibody

Alternative names for DIAPH2 antibody

DIA, diaphanous homolog 2, Diaphanous-related formin-2, DRF

SwissProt ID

O60879 (Human), O70566 (Mouse)

Gene ID

1730 (DIAPH2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Flow Cytometry (F), Enzyme Immunoassay (E)

Background of DIAPH2 antibody

DIA-2 (also called DIA drome, Dia2, DIAPH2, Diaphanous 2, Diaphanous related formin 2, Diaphorase 2, DRF2, POF and POF2) may play a role in the development and normal function of the ovaries. Mutations of this gene have been linked to premature ovarian failure. Alternative splicing results in two protein isoforms. Furthermore, each splice variant undergoes additional splicing in the 3' UTR. Overall 4 splice variants have been described. DIA-2 is expressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes and can be found from E16 in ovary and testis and during P6-P16 during differentiation of ovarian follicles. Isoform 1 is referred to as DIA-156, whereas isoform 2 is called DIA-12C.

General readings

Olsen, J.V., et al. Cell 127(3):635-648 (2006).
Olsen, J.V., et al. Cell 127(3):635-648 (2006).
Yasuda, S., et al. Nature 428(6984):767-771 (2004).
Gasman, S., et al. Nat. Cell Biol. 5(3):195-204 (2003).
Satoh, S., et al. J. Biol. Chem. 276(42):39290-39294 (2001).

10 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DIAPH2 (Isoform 2: DIA-156) antibody

WB using Anti-DIA-2 antibody shows detection of a 132-kDa band corresponding to DIA-2 in a lysate prepared from human derived HEK293 cells. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-DIA-2 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DIAPH2 (Center) antibody

Formalin-fixed and paraffin-embedded human testis tissue reacted with DIAPH2 Antibody Cat.-No AP51260PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DIAPH2 antibody

Western blot using DIAPH2 polyclonal antibody ( Cat # PAB10201 ) shows detection of a 132 kDa band corresponding to DIAPH2 in a lysate prepared from human derived HEK293 cells.
Approximately 20 µg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of DIAPH2 polyclonal antibody.
Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature.
Achemiluminescence system was used forsignal detection using a 1 min exposure time. Hu, Ms
0.1 mg / €560.00
  Abnova Taiwan Corp.

DIAPH2 antibody

Western blot analysis of DIAPH2 expression in A431 (A); HCT116 (B); SP2/0 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

DIAPH2 antibody

Western blot analysis of DIAPH2 expression in A431 (A); HCT116 (B); SP2/0 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

DIAPH2 antibody

Western blot analysis of DIAPH2 expression in A431 (A); HCT116 (B); SP2/0 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Lysates

Catalog No.    

DIAPH2 overexpression lysate

DIAPH2 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, DIAPH2 (mGFP-tagged)-Human diaphanous homolog 2 (Drosophila) (DIAPH2), transcript variant 156, 200 uL, >10^7 TU/mL

Lenti ORF particles, DIAPH2 (mGFP-tagged)-Human diaphanous homolog 2 (Drosophila) (DIAPH2), transcript variant 156, 200 uL, >10^7 TU/mL  
200 µl / €2,200.00
  OriGene Technologies, Inc.

Lenti ORF particles, DIAPH2 (Myc-DDK-tagged)-Human diaphanous homolog 2 (Drosophila) (DIAPH2), transcript variant 12C, 200 uL, >10^7 TU/mL

Lenti ORF particles, DIAPH2 (Myc-DDK-tagged)-Human diaphanous homolog 2 (Drosophila) (DIAPH2), transcript variant 12C, 200 uL, >10^7 TU/mL  
200 µl / €1,980.00
  OriGene Technologies, Inc.

Lenti ORF particles, DIAPH2 (Myc-DDK-tagged)-Human diaphanous homolog 2 (Drosophila) (DIAPH2), transcript variant 156, 200 uL, >10^7 TU/mL

Lenti ORF particles, DIAPH2 (Myc-DDK-tagged)-Human diaphanous homolog 2 (Drosophila) (DIAPH2), transcript variant 156, 200 uL, >10^7 TU/mL  
200 µl / €1,980.00
  OriGene Technologies, Inc.

10 Item(s)

per page
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