DIAPH2 antibody

Principal name

DIAPH2 antibody

Alternative names for DIAPH2 antibody

DIA, diaphanous homolog 2, Diaphanous-related formin-2, DRF

SwissProt ID

O60879 (Human), O70566 (Mouse)

Gene ID

1730 (DIAPH2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of DIAPH2 antibody

Dia 1 (also designated p140mDia) and Dia 2 (also designated p134mDia 2), mammalian homologs of the Drosophila diaphanous gene, belong to a family of formin homology (FH) proteins which are characterized by having tandemly aligned FH1 and FH2 domains in their carboxy terminal regions. The amino terminus of inactivated Dia 1 binds to the FH1 and FH2 domains and is activated when GTP-bound Rho disrupts the intramolecular interaction of Dia 1 and converts it to an open conformation. Once activated, Dia 1 co-localizes with Profilin and RhoA in dynamic plasma membrane structures and induces the formation of stress fibers in conjunction with the ROCK-mediated signaling pathway. However, Dia 1 and ROCK activities antagonize each other in order to determine the thickness and density of the stress fibers formed. Dia 1 has also been implicated as the gene responsible for non-syndromic deafness.

General readings

Olsen, J.V., et al. Cell 127(3):635-648 (2006).
Olsen, J.V., et al. Cell 127(3):635-648 (2006).
Yasuda, S., et al. Nature 428(6984):767-771 (2004).
Gasman, S., et al. Nat. Cell Biol. 5(3):195-204 (2003).
Satoh, S., et al. J. Biol. Chem. 276(42):39290-39294 (2001).

9 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DIAPH2 (Isoform 2: DIA-156) antibody

WB using Anti-DIA-2 antibody shows detection of a 132-kDa band corresponding to DIA-2 in a lysate prepared from human derived HEK293 cells. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-DIA-2 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DIAPH2 (Center) antibody

Formalin-fixed and paraffin-embedded human testis tissue reacted with DIAPH2 Antibody Cat.-No AP51260PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DIAPH2 antibody

Western blot using DIAPH2 polyclonal antibody ( Cat # PAB10201 ) shows detection of a 132 kDa band corresponding to DIAPH2 in a lysate prepared from human derived HEK293 cells.
Approximately 20 µg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of DIAPH2 polyclonal antibody.
Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature.
Achemiluminescence system was used forsignal detection using a 1 min exposure time. Hu, Ms
0.1 mg / €560.00
  Abnova Taiwan Corp.

DIAPH2 antibody

Western blot analysis of DIAPH2 expression in A431 (A); HCT116 (B); SP2/0 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

DIAPH2 antibody

Western blot analysis of DIAPH2 expression in A431 (A); HCT116 (B); SP2/0 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

DIAPH2 antibody

Western blot analysis of DIAPH2 expression in A431 (A); HCT116 (B); SP2/0 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

DIAPH2 antibody

Detection of human diaphanous 2 in HEK293 whole cell lysates (50 ug) using NB 600-280. Rabbit Aff - Purified Hu WB
0.1 mg / €440.00
  Novus Biologicals Inc.

DIAPH2 antibody

Immunohistochemistry-Paraffin: DIAPH2 Antibody [NBP1-85217] - Immunohistochemical staining of human testis shows cytoplasmic positivity in cells of seminiferus ducts. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Lysates (Overexpressed)

Catalog No.    

DIAPH2 overexpression lysate

DIAPH2 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

9 Item(s)

per page
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