Dickkopf-4 (DKK4) antibody

Principal name

Dickkopf-4 (DKK4) antibody

Alternative names for Dickkopf-4 (DKK4) antibody

Dickkopf-related protein 4, Dkk-4

SwissProt ID

Q8VEJ3 (Mouse), Q9UBT3 (Human)

Gene ID

27121 (DKK4), 5742 (PTGS1), 234130 (Dkk4)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Por (Porcine)

Available hosts

Rabbit, Goat, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Enzyme Immunoassay (E)

Background of Dickkopf-4 (DKK4) antibody

DKK4, like DKK1, DKK2, and DKK3, possesses an N terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T cell, esophagus, and lung cDNA libraries. DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors.
DKK4, like DKK1, DKK2, and DKK3, possesses an N terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T cell, esophagus, and lung cDNA libraries. DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors.

General readings

Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853 (2009).
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049 (2009).
Hirata, H., et al. Cancer 115(19):4488-4503 (2009).
Matsui, A., et al. Cancer Sci. 100(10):1923-1930 (2009).
Baehs, S., et al. Cancer Lett. 276(2):152-159 (2009).

19 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Dickkopf-4 (DKK4) (C-term) antibody

TA302466 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Dickkopf-4 (DKK4) antibody

Western blot analysis of Dkk4 expression in Jurkat cell lysate. Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Dickkopf-4 (DKK4) (N-term) antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with DKK4 antibody (N-term) (AP11556PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Dickkopf-4 (DKK4) (C-term) antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Dickkopf-4 (DKK4) (C-term) antibody

Western blot analysis of DKK4 Antibody (C-term) Cat. AP51270PU-N in K562,HL-60, MDA-MB453 cell line lysates (35ug/lane). This demonstrates the DKK4 antibody detected the DKK4 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Dickkopf-4 (DKK4) antibody

Dickkopf-4 (DKK4) Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Dickkopf-4 (DKK4) antibody

Western Blot analysis of DKK4 expression in transfected 293T cell line (H00027121-T02) by DKK4 MaxPab polyclonal antibody.

Lane 1: DKK4 transfected lysate(24.90 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Dickkopf-4 (DKK4) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with DKK4 polyclonal antibody ( Cat # PAB3573 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

Dickkopf-4 (DKK4) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with DKK4 polyclonal antibody ( Cat # PAB3574 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

Dickkopf-4 (DKK4) antibody

DKK4 polyclonal antibody ( Cat # PAB6130 ) staining ( 1 µg/ml ) of Jurkat lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Dickkopf-4 (DKK4) antibody

Western blot analysis of Dkk4 expression in Jurkat cell lysate. Rabbit Hu, Ms, Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Dickkopf-4 (DKK4) antibody

NB 100-1000 staining (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Dickkopf-4 (DKK4) antibody

Western Blot: DKK4 Antibody [NBP1-45523] - Western blot analysis of Dkk4 expression in Jurkat cell lysate. Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Dickkopf-4 (DKK4)

Dickkopf-4 (DKK4) Human
2 µg / €300.00
  Abnova Taiwan Corp.

Dickkopf-4 (DKK4)

Dickkopf-4 (DKK4) Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Dickkopf-4 (DKK4)

Dickkopf-4 (DKK4) Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

ELISA Kits

Catalog No.    

DKK4 (Human) ELISA Kit

DKK4 (Human) ELISA Kit  
1 kit / €590.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

DKK4 293T Cell Transient Overexpression Lysate(Denatured)

DKK4 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

DKK4 overexpression lysate

DKK4 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

19 Item(s)

per page
  • LinkedIn