DISC1 antibody

Principal name

DISC1 antibody

Alternative names for DISC1 antibody

DISC-1, KIAA0457, Disrupted in schizophrenia 1 protein

SwissProt ID

Q810H6 (Rat), Q811T9 (Mouse), Q9NRI5 (Human)

Gene ID

307940 (Disc1), 244667, 27185 (DISC1), 827185 (BAM5)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Eq (Equine), Rb (Rabbit), Por (Porcine)

Available hosts

Rabbit, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F)

Background of DISC1 antibody

The genetic and epidemiological studies suggest that schizophrenia is largely due to individual variation and susceptibility of different alleles with small to moderate effects on multiple genes. Molecular genetic studies have identified several potential regions of linkage and 2 associated chromosomal abnormalities in Schizophrenia and suggest several positional candidate genes including Disrupted in Schizophrenia 1 (DISC1). DISC1 is linked to Schizophrenia by association and linkage studies in independent population and is the only gene whole open reading frame (ORF) is truncated and co segregates with mojor mental illnesses in a Scottish population. It is well known that several neuronal pathways are disrupted in schizophrenia, the complexity of the genetics underlying schizophrenia is highlighted by the multitude of molecular pathways that have been reported to be disrupted in the disorder including muscarinic, serotonergic, and glutamatergic signaling systems. The genetic and epidemiological studies suggest that schizophrenia is largely due to individual variation and susceptibility of different alleles with small to moderate effects on multiple genes. Molecular genetic studies have identified several potential regions of linkage and 2 associated chromosomal abnormalities in Schizophrenia and suggest several positional candidate genes including Disrupted in Schizophrenia 1 (DISC1). Recent epigenetic and molecular biology data strongly suggest Disrupted in Schizophrenia 1 (Disc 1) gene as a risk factor of great significance in the underlying causes of Schizophrenia and related disorders. No functional significance of this protein was evident despite the presence of several well characterized protein domains on Disc 1. Recently the discovery and identification of its binding partners has revealed an incredible diversity of potential cellular and physiological functions. The interactive yeast-two hybrid screening revealed the “Disc 1 interactome” which contained several novel protein-protein interaction sites suggesting its role in several diverse functions. Studies on Disc 1 interactome showed protein-protein interaction with ligands that are consistent with the underlying molecular pathology observed at the synaptic level and the cognitive deficits seen behaviorally in schizophrenics (1). Phosphodiesterase Type 4B (PDE4B) and Ndell-EOPA are two known target proteins that actively interact with Disc 1 (2).
The genetic and epidemiological studies suggest that schizophrenia is largely due to individual variation and susceptibility of different alleles with small to maderate effects on multiple genes. Molecular genetic studies have identified several potential regions of linkage and 2 associated chromosomal abnormalities in Schizophrenia and suggest several positional candidate genes including Disrupted in Schizophrenia 1 (DISC1). DISC1 is linked to Schizophrenia by association and linkage studies in independent population and is the only gene whole open reading frame (ORF) is truncated and co segregates with major mental illnesses in a Scottish population. A recent attempt to study and localize DISC1 in human autopsied brain samples from patients with schizophrenia, bipolar disorder, and major depression. GenerallyDISC1 is localized in nucleus of HeLa cells, however, this localization was altered in orbitofrontal cortex significantly in all major mental illnesses including substance and alcohol abuse suggesting DISC1 may be implicated in psychiatric conditions (1). Disc1 transgenic mice displayed several behavioral abnormalities including hyperactivity, disturbance in sensorimotor gating and olfactory and olfactory-associated behaviors and anhedonia and depression like symptoms (2). Several single nucleotide polymorphisms (SNPs) in DISC1 gene were typed, a three-SNP halotype spanning 83 kb of the gene was associated with schizophrenia in family-based sample 93). Disc 1 was found in hippocampus where allelic variation in Disc 1at Ser704Cys would have measurable impact on hippcampal structure and function including reduced hippocampal gray matter volume and altered engagement of hippocampus during several cognitive tasks assays (3). Disc1 is 832 amino acid protein with predicted MW of around 100kDa. In brain Disc1 is expressed as two distinct bands of molecular weight 95-100kDa and 70-85 kDa in human brain. This patters is similar in rodent brain where 70-85kDa band intensity is much more intense than higher MW species. The identity of the 70-85 kDa species is still not yet confirmed.

General readings

Millar JK, Pickard BS, Mackie S, James R, Christie S, Buchanan SR, Malloy MP, Chubb JE, Huston E, Baillie GS, Thomson PA, Hill EV, Brandon NJ, Rain JC, Camargo LM, Whiting PJ, Houslay MD, Blackwood DH, Muir WJ, Porteous DJ.
DISC1 and PDE4B are interacting genetic factors in schizophrenia that regulate cAMP signaling.
Science. 2005 Nov 18;310(5751):1187-91.
PMID: 16293762

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DISC1 antibody

TA302463 (0.1µg/ml) staining of human brain (hippocampus) lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DISC1 (N-term) antibody

Western blot analysis of DISC1 in 3T3 cell lysate with DISC1 antibody at (A) 1 and (B) 2 µg/ml. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DISC1 antibody

Western blot analysis of DISC1 in SK-N-SH cell lysate with DISC1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DISC1 antibody

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using DISC1 Antibody at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DISC1 antibody

Predicted cell location: Cytoplasm. Positive control: Human brain tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using DISC1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

DISC1 antibody

Immunohistochemistry: DISC1 Antibody [TA336517] - Staining of DISC1 in human brain neurons and neuropil at 2.5ug/ml. 40X. Rabbit Purified Hu, Ms, Rt C, ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

DISC1 antibody

Immunohistochemistry: DISC1 Antibody [TA336518] - Staining of DISC1 in mouse brain neurons, glial cells, and white matter. 40X Rabbit Purified Ms, Rt C, ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

DISC1 antibody

WB Suggested Anti-DISC1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate;There is BioGPS gene expression data showing that DISC1 is expressed in Jurkat Rabbit IgG Purified Eq, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

DISC1 antibody

WB Suggested Anti-DISC1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Hela cell lysate Rabbit IgG Purified Eq, Hu, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

DISC1 antibody

Immunocytochemical staining of HeLa cells with Rabbit anti Human DISC1 (AP05716PU-N) Rabbit IgG Purified Hu, Ms ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

DISC1 (C-term) antibody

AP08905PU-N DISC1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex at 10-20 µg/ml. Rabbit IgG Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

DISC1 (C-term) antibody

Brain, cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

DISC1 (C-term) antibody

Immunohistochemistry: AP08999PU-N DISC1 Antibody staining of Formalin-Fixed Paraffin-Embedded of Human Brain, cerebellum at 5 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Aff - Purified Ms E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

DISC1 Control Peptide

DISC1
0.25 mg / €250.00
  Acris Antibodies GmbH

DISC1 (743-749) antibody

DISC1 Rabbit Aff - Purified Hu E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

DISC1 Control Peptide

DISC1
50 µg / €160.00
  Acris Antibodies GmbH

DISC1 Control Peptide

DISC1
50 µg / €160.00
  Acris Antibodies GmbH

DISC1 (C-term) antibody

Formalin fixed and paraffin embedded human brain tissue reacted with DISC1 Antibody (C-term) Cat.-No AP51267PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DISC1 antibody

DISC1 Rabbit Hu E, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

DISC1 antibody

DISC1 Rabbit Hu E, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

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