Dishevelled-2 / DVL2 antibody
Principal name
Dishevelled-2 / DVL2 antibody
Alternative names for Dishevelled-2 / DVL2 antibody
DVL-2, DSH homolog 2, Segment polarity protein dishevelled homolog DVL-2
Gene ID
1856 (DVL2)
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Enzyme Immunoassay (E)
Background of Dishevelled-2 / DVL2 antibody
DVL2 is a member of the dishevelled (dsh) protein family. It is a 90-kD protein that undergoes posttranslatiol phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the sigl transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt sigling are likely to be conserved among metazoans. This gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslatiol phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the sigl transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt sigling are likely to be conserved among metazoans. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications.
General readings
Inkster, B., et al. Neuroimage 53(3):908-917(2010).
Kikuchi, K., et al. EMBO J. 29(20):3470-3483(2010).
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010).
Gao, C., et al. Nat. Cell Biol. 12(8):781-790(2010).
Gnad, T., et al. Mol. Cancer 9, 31 (2010).
Western Blot analysis of DVL2 expression in transfected 293T cell line (H00001856-T01) by DVL2 MaxPab polyclonal antibody.Lane 1: DVL2 transfected lysate(80.96 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of DVL2 transfected lysate using anti-DVL2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with DVL2 MaxPab mouse polyclonal antibody (B01) (H00001856-B01).
Human small intestine; . IHC Information: Paraffin embedded small intestine, muscularis propria tissue, tested with an antibody Dilution of 5 ug/ml.; DVL2 antibody - N-terminal region (ARP32102_P050) in Human small intestine cells using Immunohistochemistry
Transfected 293T; WB Suggested Anti-DVL2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; DVL2 antibody - N-terminal region (ARP32102_P050) in Transfected 293T cells using Western Blot
Western blot analysis of Dishevelled-2 / DVL2 Antibody (C-term) Cat.-No AP51345PU-N in CEM cell line lysates (35ug/lane). This demonstrates the DVL2 antibody detected the DVL2 protein (arrow).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DVL2 (RC202233, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DVL2.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DVL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806789)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DVL2 (RC202233, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DVL2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DVL2 (RC202233, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DVL2.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-DVL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806791)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-DVL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806791)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-DVL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806791)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DVL2 (RC202233, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DVL2.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-DVL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806917)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DVL2 (RC202233, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DVL2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DVL2 (RC202233, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DVL2.
Western blot analysis of extracts from K562/Jurkat cells, using DVL2 antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-DVL2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Transfected 293T
IHC Information: Paraffin embedded small intestine, muscularis propria tissue, tested with an antibody Dilution of 5 ug/ml.
Host: Rabbit; Target Name: DVL2; Sample Tissue: Thymus Tumor lysates; Antibody Dilution: 1.0ug/ml
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (DVL2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using (DVL2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA806788 | Dishevelled-2 / DVL2 antibody |
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| DVL2 mouse monoclonal antibody, clone OTI7A3 (formerly 7A3) | |||||||
 |
Mouse | IgG1 | OTI7A3 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA806789 | Dishevelled-2 / DVL2 antibody |
||||||
| DVL2 mouse monoclonal antibody, clone OTI7C7 (formerly 7C7) | |||||||
 |
Mouse | IgG1 | OTI7C7 | Purified | Hu | P, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|
||||||
| TA806790 | Dishevelled-2 / DVL2 antibody |
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| DVL2 mouse monoclonal antibody, clone OTI1E11 (formerly 1E11) | |||||||
 |
Mouse | IgG1 | OTI1E11 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA806791 | Dishevelled-2 / DVL2 antibody |
||||||
| DVL2 mouse monoclonal antibody, clone OTI2D11 (formerly 2D11) | |||||||
 |
Mouse | IgG2b | OTI2D11 | Purified | Hu | P |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA806832 | Dishevelled-2 / DVL2 antibody |
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| DVL2 mouse monoclonal antibody, clone OTI1B12 (formerly 1B12) | |||||||
 |
Mouse | IgG1 | OTI1B12 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA806917 | Dishevelled-2 / DVL2 antibody |
||||||
| DVL2 mouse monoclonal antibody, clone OTI2E11 (formerly 2E11) | |||||||
 |
Mouse | IgG1 | OTI2E11 | Purified | Hu | P, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA806918 | Dishevelled-2 / DVL2 antibody |
||||||
| DVL2 mouse monoclonal antibody, clone OTI7F10 (formerly 7F10) | |||||||
 |
Mouse | IgG2a | OTI7F10 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
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