DJ-1 / PARK7 antibody

Principal name

DJ-1 / PARK7 antibody

Alternative names for DJ-1 / PARK7 antibody

Oncogene DJ1, Parkinson disease protein 7

SwissProt ID

O88767 (Rat), Q5E946 (Bovin), Q8UW59 (Chick), Q99497 (Human), Q99LX0 (Mouse)

Gene ID

11315 (PARK7), 57320 (Park7), 117287

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Chk (Chicken), Hst (Hamster), Rt (Rat), Ze (Zebrafish), Can (Canine), Fi (Fish), Frog, Chimp (Chimpanzee), Mky (Monkey), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of DJ-1 / PARK7 antibody

DJ-1 (PARK7/CAP1/RS) was originally cloned as a putative oncogene capable of transforming NIH/3T3 cells in cooperation with h-ras, a protein expressed in sperm, and a regulator of RNA-protein interactions. DJ-1 has also been isolated as a gene associated with autosomal ea rly-onset Parkinson's disease (PD). Taken together, DJ-1 appears to be involved in diverse biological processes. First, several lines of evidence suggest that DJ-1 plays a role in the oxidative stress response. In cultured mammalian cells, DJ-1 quenches reactive oxygen species and is converted into a variant with a more acidic isoelectric poin t. Therefore, DJ-1 protects against reactive oxygen specie s-induced cell death, and its suppression with small interfe ring RNA (siRNA) sensitizes cells to such insults. Second, DJ-1 modulates transcription through interaction with DJ-1-binding protein as well as with protein inhibitor of activated STAT (PIAS). The latter modulates the activity of various transcription factors. Third, DJ-1 has been recognized as a regulatory subunit of an RNA-binding protein. Fourth, DJ-1, which is structurally related to the molecular chaperone Hsp31, may have chaperone activity itsel f, preventing heat-induced aggregation of substrate proteins, including α -synuclein. In addition, several lines of evidence suggest that DJ-1 plays a role in human tumorigenesis. First, breast cancer patients have elevated levels of circulating DJ-1 and anti-DJ-1 autoantibodies compared to healthy and non-breast cancer patients. Secondly, DJ-1 protein is increased in primary non-small cell lung carcinoma sa mples. Thirdly, treatment of cells from the human lung cancer cell line NCI-H157 with paclitaxel and MEK inhibitor U0126 leads to a decrease in DJ-1 protein expression.

General readings

Willis at al, Differential transport and local translation of cytoskeletal, injury-response, and neurodegeneration protein mRNAs in axons. J Neurosci. 2005 Jan 26;25(4):778-91.
PMID: 15673657

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DJ-1 / PARK7 antibody

Western blot analysis of extracts from HuvEc cells, using DJ-1 antibody (#TA326226).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DJ-1 / PARK7 antibody

Human breast stained with DJ-1 antibody Rabbit Purified Hu, Ms P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

DJ-1 / PARK7 antibody

Human breast stained with DJ-1 antibody Rabbit Purified Hu, Ms P, WB
1 ml / €520.00
  Acris Antibodies GmbH

DJ-1 / PARK7 antibody

Human breast stained with DJ-1 antibody Rabbit Purified Hu, Ms P, WB
0.1 ml / €190.00
  Acris Antibodies GmbH

DJ-1 / PARK7 (C-term) antibody

TA302679 (0.05 ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt E, WB
20 µg / €200.00
  Acris Antibodies GmbH

DJ-1 / PARK7 antibody

Western Blot analysis of DJ-1 protein in Hek293 cell lysates Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DJ-1 / PARK7 (C-term) antibody

TA302679 (0.05 ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DJ-1 / PARK7 antibody

Western Blot: PARK7 Antibody - Western analysis of PARK7 (DJ-1) in wild-type and DJ-1 knockout mice antibody at 1:1,000 dilution. Beta actin is shown as control. Rabbit Purified Hu C, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DJ-1 / PARK7 antibody

Predicted band size: 20 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/200-1000. (Gel: %SDS-PAGE Lysate: 60 ug per lane Primary antibody: 1/300dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DJ-1 / PARK7 antibody

Predicted band size: 20 kDa . Positive control: Hela and HT-29 cell lysate. Recommended dilution: 1/200-1000. (Gel: %SDS-PAGE Lysate: 60 ug per lane Primary antibody: 1/200dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DJ-1 / PARK7 antibody

Western blot analysis of Jurkat cell lysate using PAPK7 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

DJ-1 / PARK7 antibody

Western blot analysis on HuvEc cell lysate using PARK7 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DJ-1 / PARK7 (1-187) antibody

Western blot analysis of DJ-1 expression in Jurkat (1), Raji (2), HeLa (3), NIH/3T3 (4) and PC12 (5) using AM26596AF-N Mouse IgG1 3E8 Azide Free Hu WB
0.1 mg / €380.00
  Acris Antibodies GmbH

DJ-1 / PARK7 antibody

Immunofluorescence of HeLa cells stained with anti-park7 (green) showing strong cytoplasmic staining and anti-vimentin antibody (red). Mouse IgG1 4H4 Purified Bov, Hu, Ms, Rt ICC/IF, WB
0.1 ml / €380.00
  Acris Antibodies GmbH

DJ-1 / PARK7 antibody

The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARK7 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT1E12 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

DJ-1 / PARK7 antibody

The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARK7 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT1E12 Purified Hu E, F, ICC/IF, WB
50 µl / €220.00
  Acris Antibodies GmbH

DJ-1 / PARK7 antibody

Western blot analysis of DJ-1 Antibody (Cat.-No AP01249PU-N) in extracts from HT-29 cells. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

DJ-1 / PARK7 antibody

Immunohistochemical analysis in paraffin-embedded human lung carcinoma tissue using DJ-1 / PARK7 antibody (Cat.-No. AP06086PU-N). Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

DJ-1 / PARK7 (177-189) antibody

Pancreas: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Can, Chk, Fi, Frog, Hu, Mky, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

DJ-1 / PARK7 (177-189) antibody

Immunohistochemistry. Anti-PARK7 antibody was used at a 5 μg/ml to detect PARK7 in a variety of tissues. In some tissues elevated background staining was noted. In these instances further optimization of dilution is suggested. This image shows PARK7 staining of human pancreas. Tissue was formalinfixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA. Rabbit IgG Aff - Purified Chimp, Hu, Mky, Ze E, P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

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