DLG1 antibody

Principal name

DLG1 antibody

Alternative names for DLG1 antibody

Disks large homolog 1, Synapse-associated protein 97, SAP-97

SwissProt ID

Q12959 (Human), Q62696 (Rat), Q811D0 (Mouse)

Gene ID

1739 (DLG1), 25252 (Dlg1)

Available reactivities

Rt (Rat), Ms (Mouse), Hu (Human)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of DLG1 antibody

Synapse-Associated Protein 97 (SAP97/human homologue of Drosophila discs-large tumor supressor or HDLG) is one of a family of plasma membrane-associated proteins found in synaptic junctions. This is a novel, presynaptic membrane protein homologous to SAP90 and the Drosophila discs-large tumor supressor protein. SAP97 has three ~90 amino acid repeats called PDZ domains, a single interior SH3 domain, and a carboxyl-terminal guanylate kinase homology (GuK) domain that is enzymatically inactive. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticity.
SAP97 is believed to participate in the clustering of certain proteins, including N-methyl-D-aspartate (NMDA) receptors and Shaker-type potassium channels at the synaptic membrane. There are two principal modes of interaction between SAP97 and other proteins. NMDA receptors and Shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with SAP97 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with SAP97 in vitro through a pseudo-homotypic PDZ-PDZ interaction.

General readings

General / background references:Gomperts SN Clustering membrane proteins: It's all coming together with the PSD-95/SAP90 protein family. Cell 84:659-62 (1996). PubMed PMID: 8625403
Muller BM et al. Molecular characterization and spatial distribution of SAP97, a novel presynaptic protein homologous to SAP90 and the Drosophila discs-large tumor suppressor protein. J Neurosci 15:2354-66 (1995). PubMed PMID: 7891172
Cho KO et al. The rat brain postsynaptic density fraction contains a homolog of the Drosophila discs-large tumor suppressor protein. Neuron 9:929-42 (1992). PubMed PMID: 1419001
1. Gardner, L. A., Naren, A. P., and Bahouth, S. W. (2007) Assembly of an SAP97-AKAP79-cAMP-dependent Protein Kinase Scaffold at the Type 1 PSD-95/DLG/ZO1 Motif of the Human beta1-Adrenergic Receptor Generates a Receptosome Involved in Receptor Recycling and Networking, 282, 5085-5099.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DLG1 antibody

Adult rat hippocampus immunohistochemistry. Mouse IgG1 K64/15 Supernatant Hu, Ms, Rt C, IP, WB
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

DLG1 antibody

Adult rat hippocampus immunohistochemistry. Mouse IgG1 K64/15 Purified Hu, Ms, Rt C, IP, WB
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

DLG1 antibody

TA305843 (0.3ug/ml) staining of KELLY lysate (35ug protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DLG1 antibody

Western blot analysis of SAP97 on rat brain membrane tissues using a 1:1000 dilution of the antibody Mouse IgG1 S64-15 Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DLG1 (1-104) antibody

DLG1 Mouse IgG1 S64-15 Purified Hu, Ms, Rt C, ICC/IF, IP, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

DLG1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between LCK and DLG1. HeLa cells were stained with anti-LCK rabbit purified polyclonal 1:1200 and anti-DLG1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 1D8 Purified Hu E, WB
50 µg / €370.00
  Abnova Taiwan Corp.

DLG1 antibody

DLG1 Mouse IgG2a 1D8 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

DLG1 antibody

DLG1 Mouse IgG1 1B12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DLG1 antibody

DLG1 polyclonal antibody ( Cat # PAB11517 ) ( 0.3 µg/ml ) staining of Kelly lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

DLG1 antibody

DLG1 Rabbit IgG Purified Rt C, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

DLG1 antibody

Western Blot: DLG1 Antibody [NBP1-20907]-(0.3ug/ml) staining of KELLY lysate (35ug protein in RIPA buffer). Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

DLG1 antibody

Western Blot: SAP97 Antibody [NBP1-26611]-Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells.  Affinity purified rabbit anti-SAP97 used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 10 mcg/mg lysate.  SAP97 was also immunoprecipitated by another rabbit anti-SAP97 antibody, which recognizes a downstream epitope. Rabbit IgG Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

DLG1 antibody

Immunohistochemistry-Paraffin: SAP97 Antibody (S64-15) [NBP1-48054] - IHC analysis of SAP97 in frozen sections of mouse brain extract using NBP1-48054. Mouse IgG1 S64-15 Purified Hu, Ms, Rt C, IP, P, WB
0.1 mg / €450.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DLG1

DLG1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

DLG1

DLG1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

DLG1 overexpression lysate

DLG1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

16 Item(s)

per page
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