DLK1 antibody

Principal name

DLK1 antibody

Alternative names for DLK1 antibody

DLK-1, DLK, Protein delta homolog 1, pG2, PREF1, Preadipocyte factor 1

SwissProt ID

P80370 (Human), Q09163 (Mouse)

Gene ID

8788 (DLK1), 13386 (Dlk1)

Available reactivities

Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Can (Canine), Eq (Equine), GP (Guinea Pig), Sh (Sheep)

Available hosts

Mouse, Rabbit, Rat

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of DLK1 antibody

Delta like protein (Dlk), also known as Preadipocyte factor-1 (Pref-1) or zona glomerulosa-specific factor (ZOG), is an EGF-like transmembrane protein expressed preadipocytes but not in mature adipocytes. It is highly expressed in fetal liver, the adrenal gland, and placenta, as well as some neuroendocrine tumors and small cell lung carcinomas, where it plays a role in differentiation and proliferation. Dlk positively and negatively regulates adipocyte differentiation via at least four major variants (45-60 kDa) of Dlk generated by alternatively splicing. Constitutive expression of Dlk inhibits adipogenesis, but insulin or insulin like growth factor-1 (IGF-1) can circumvent this inhibition. Regulated processing of Dlk releases a 50 kDa soluble form that was previously characterized as Fetal Antigen-1, a protein involved in pancreatic island cell differentiation.

General readings

1. Yanai, H. et al., J. Biochem. 148(1): 85-92 (2010).
2. Yu, F. et al., Liver Int. 30(5): 703-714 (2010).
3. Akiyama, J. et al., Biochem. Biophys. Res. Commun. 393(4): 662-667 (2010).
4. Wermter, A.K. et al., Eur. J. Hum. Genet. 16(9): 1126-1134 (2008).
5. Charlier, C. et al., Genome Res. 11(5): 850-862 (2001).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DLK1 antibody

Immunofluorescence analysis of U251 cells using DLK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Mouse IgG1 3A10 Ascites Hu E, F, ICC/IF, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

DLK1 (C-term) antibody

  Rabbit Polyclonal DLK1 Antibody  
Western blot analysis of HepG2 in 293 cell lysate with DLK1 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DLK1 antibody

  Rabbit Polyclonal Anti-DLK1 Antibody  
WB Suggested Anti-DLK1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human Stomach Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Por, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

DLK1 antibody

Immunohistochemical detection of Dlkon paraffin embedded section of mouse fetal liver (E14.5) with AM26511AF-N. Rat IgG1 24-11 Azide Free Ms C, F, P
0.1 mg / €370.00
  Acris Antibodies GmbH

DLK1 antibody

lFlow Cytometric analysis of Dlkexpression on mouse fetal liver cells (E14) using isotype control (left) and AM26511FC-N. Rat IgG1 24-11 FITC Ms F
0.1 ml / €370.00
  Acris Antibodies GmbH

DLK1 antibody

Flow cytometric analysis of Dlk expression on mouse Fetal Hepatocytes. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26511RP-N to the cells. Rat IgG1 24-11 PE Ms F
50 Tests / €400.00
  Acris Antibodies GmbH

DLK1 antibody

Immunohistochemistry analysis in human placenta tissue (Formalin-fixed, Paraffin-embedded) using DLK1 antibody Cat.-No. AP51272PU-N, followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of DLK1 antibody for IHC. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DLK1 antibody

Western Blot analysis of DLK1 expression in transfected 293T cell line (H00008788-T02) by DLK1 MaxPab polyclonal antibody.

Lane 1: DLK1 transfected lysate(42.13 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

DLK1 antibody

DLK1 MaxPab rabbit polyclonal antibody. Western Blot analysis of DLK1 expression in mouse liver. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

DLK1 antibody

Human Stomach; WB Suggested Anti-DLK1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Stomach; DLK1 antibody - middle region (ARP46375_P050) in Human Stomach cells using Western Blot Rabbit Aff - Purified Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

DLK1 - human gene knockout kit via CRISPR

DLK1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

DLK1 - human gene knockout kit via CRISPR

DLK1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

DLK1 - human gene knockout kit via CRISPR

DLK1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

DLK1 - human gene knockout kit via CRISPR

DLK1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dlk1 - mouse gene knockout kit via CRISPR

Dlk1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Dlk1 - mouse gene knockout kit via CRISPR

Dlk1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dlk1 - mouse gene knockout kit via CRISPR

Dlk1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dlk1 - mouse gene knockout kit via CRISPR

Dlk1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DLK1

  Recombinant protein of human delta-like 1 homolog (Drosophila) (DLK1)  
DLK1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

DLK1

  Recombinant protein of human delta-like 1 homolog (Drosophila) (DLK1)  
DLK1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €299.00
  OriGene Technologies, Inc.

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