DNA ligase 1 antibody

Principal name

DNA ligase 1 antibody

Alternative names for DNA ligase 1 antibody

LIG1, LIG-1, DNA ligase 1, EC=6.5.1.1, DNA ligase I, Polydeoxyribonucleotide synthase [ATP] 1

SwissProt ID

P18858 (Human), P37913 (Mouse), Q9JHY8 (Rat)

Gene ID

3978 (LIG1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, GP (Guinea Pig), Rb (Rabbit), Eq (Equine), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of DNA ligase 1 antibody

Eukaryotic DNA ligases are ATP-dependent enzymes that catalyse the joining of single and double-strand DNA breaks, which is an essential final step in DNA replication, recombination and repair. Four biochemically distinct DNA ligases, termed ligases I-IV, have been identified in mammalian cells. DNA ligase I is functionally homologous to the DNA ligase encoded by the Saccharomyces cerevisiae Cdc9 gene. The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is due to DNA ligase I. A combination of DNA polymerase epsilon, PCNA, replication factor C, replication protein A, and DNA ligase I is well-suited to the task of creating nucleotide excision repair patches.

General readings

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Arora, M., et al. Leukemia 24(8):1470-1475(2010)
Guillem, V.M., et al. Am. J. Hematol. 85(7):482-486(2010)
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Monsees, G.M., et al. Breast Cancer Res. Treat. (2010) In press

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DNA ligase 1 antibody

Immunohistochemical detection of DNA Ligase I on paraffin embedded section of a human breast cancer with AM26565AF-N. Mouse IgG1 5H5 Azide Free Hu, Ms, Rt F, ICC/IF, IP, P, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

DNA ligase 1 antibody

TA305925 (0.1ug/ml) staining of HeLa nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNA ligase 1 antibody

Western blot analysis of extracts of various cell lines, using LIG1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNA ligase 1 antibody

WB Suggested Anti-LIG1 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysateLIG1 is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

DNA ligase 1 antibody

WB Suggested Anti-LIG1 Antibody Titration: 0.2-1 ug/ml; Positive Control: A549 cell lysateLIG1 is supported by BioGPS gene expression data to be expressed in A549 Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

DNA ligase 1 antibody

Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 10G12 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

DNA ligase 1 antibody

Immunohistochemistry (IHC) analyzes of DNA Ligase I antibody (Cat.-No.: AP20546PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

DNA ligase 1 (N-term) antibody

Western blot analysis of DNA liage 1 Antibody (N-term) (Cat.-No AP52477PU-N in CEM cell line lysates (35ug/lane). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DNA ligase 1 antibody

DNA ligase 1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

DNA ligase 1 antibody

Western Blot analysis of LIG1 expression in transfected 293T cell line (H00003978-T02) by LIG1 MaxPab polyclonal antibody.

Lane 1: LIG1 transfected lysate(101.70 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

DNA ligase 1 antibody

Immunoperoxidase of monoclonal antibody to LIG1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.2 ug/ml] Mouse IgG2a 10G12 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DNA ligase 1 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using DNL1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, P, WB
50 µg / €205.00
  Assay Biotech

DNA ligase 1 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using DNL1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €310.00
  Assay Biotech

DNA ligase 1 antibody

Human HepG2; WB Suggested Anti-LIG1 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate. LIG1 is supported by BioGPS gene expression data to be expressed in HepG2.; LIG1 antibody - middle region (ARP54307_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

DNA ligase 1 (Center) antibody

Human A549; WB Suggested Anti-LIG1 Antibody Titration: 0.2-1 ug/ml. Positive Control: A549 cell lysate. LIG1 is supported by BioGPS gene expression data to be expressed in A549.; LIG1 antibody - middle region (ARP54308_P050) in Human A549 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

DNA ligase 1 antibody

Western Blot: DNA Ligase 1 Antibody [NB100-58815] - Detection of Human DNA Ligase 1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20 percent of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DNA Ligase 1 antibody NB100-58815 used for IP at 3 mcg/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds. Rabbit Aff - Purified Hu IP, P
0.1 mg / €440.00
  Novus Biologicals Inc.

DNA ligase 1 antibody

Immunohistochemistry: DNA Ligase I Antibody [NBP1-19701] -  DNA Ligase I Antibody [NBP1-19701] - paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

DNA ligase 1 antibody

DNA ligase 1 Goat Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

DNA ligase 1 antibody

DNA ligase 1 Rabbit IgG Aff - Purified Hu E, P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

DNA ligase 1 antibody

DNA ligase 1 Rabbit IgG Aff - Purified Hu E, P, WB
50 µg / €290.00
  Novus Biologicals Inc.

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