DNA ligase 4 antibody

Principal name

DNA ligase 4 antibody

Alternative names for DNA ligase 4 antibody

LIG4, EC=6.5.1.1, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4

SwissProt ID

P49917 (Human), Q8BTF7 (Mouse), Q90YB1 (Chick)

Gene ID

3981 (LIG4), 885679 (mpg)

Available reactivities

Hu (Human), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), African clawed frog, Xen (Xenopus), Bov (Bovine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of DNA ligase 4 antibody

The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Smith,J., et al., (2003) Nucleic Acids Res. 31 (8), 2157-2167

20 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DNA ligase 4 antibody

Western blot analysis of extracts from Jurkat cells, using DNL4 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNA ligase 4 antibody

Predicted band size: 104 kDa . Positive control: K562, Jurkat and MCF7 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: K562 cell lysate Lane2: Jurkat cell lysate Lane3: MCF7 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNA ligase 4 antibody

WB Suggested Anti-LIG4 Antibody Titration: 1.25 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rb, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNA ligase 4 antibody

Western blot analysis of DNA Ligase IV Antibody (Cat.-No AP06802PU-N) in extracts from Jurkat cells. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

DNA ligase 4 antibody

DNA ligase 4 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

DNA ligase 4 antibody

Detection limit for recombinant GST tagged LIG4 is approximately 1ng/ml as a capture antibody. Mouse IgG2a 1A4 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

DNA ligase 4 antibody

DNA ligase 4 Mouse IgG2a 1A4 Ascites Hu E
0.2 ml / €370.00
  Abnova Taiwan Corp.

DNA ligase 4 antibody

Detection limit for recombinant GST tagged LIG4 is approximately 1ng/ml as a capture antibody. Mouse IgG2a 2D2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DNA ligase 4 antibody

DNA ligase 4 Rabbit Aff - Purified Hu E, P
50 µg / €205.00
  Assay Biotech

DNA ligase 4 antibody

DNA ligase 4 Rabbit Aff - Purified Hu E, P
0.1 mg / €310.00
  Assay Biotech

DNA ligase 4 antibody

Western blot analysis of extracts from Jurkat cells, using DNL4 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €205.00
  Assay Biotech

DNA ligase 4 antibody

Western blot analysis of extracts from Jurkat cells, using DNL4 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €310.00
  Assay Biotech

DNA ligase 4 antibody

Human Pancreas; LIG4 antibody - N-terminal region (ARP34122_T100) in Human Pancreas cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Can, Chk, Hu, Ms, Rt P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

DNA ligase 4 antibody

Immunohistochemical analysis of DNA Ligase 4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

DNA ligase 4 antibody

Immunohistochemical analysis of DNA Ligase 4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

DNA ligase 4 antibody

Immunohistochemical analysis of DNA Ligase 4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

DNA ligase 4 antibody

Western Blot: LIG4 Antibody [NB110-57379] - Western blot on Hep G2 cell lysate stained with NB110-57379 at 1.25ug/ml. Rabbit Purified Can, Chk, Hu, Ms, Rt, Xen P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

DNA ligase 4 antibody

Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP1-87405] Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DNA ligase 4

DNA ligase 4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

DNA ligase 4

DNA ligase 4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

20 Item(s)

per page
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