DNA-PKcs / PRKDC / XRCC7 antibody

Principal name

DNA-PKcs / PRKDC / XRCC7 antibody

Alternative names for DNA-PKcs / PRKDC / XRCC7 antibody

DNPK1, p460, HYRC, HYRC1, DNA-dependent protein kinase catalytic subunit

SwissProt ID

P78527 (Human), P97313 (Mouse), Q8QGX4 (Chick), Q8WN22 (Canfa), Q9DEI1 (Xenla)

Gene ID

5591 (PRKDC), 19090 (Prkdc)

Available reactivities

Hst (Hamster), Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Chk (Chicken), Eq (Equine), Chimp (Chimpanzee)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Electrophoretic Mobility Shift Assay (EMSA), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), Frozen Sections (C)

Background of DNA-PKcs / PRKDC / XRCC7 antibody

Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, c-myc/MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate TP53/p53 in the presence of supercoiled DNA is dependent on C1D.

General readings

General / background references:Han Z et al. DNA-dependent protein kinase is a target for a CPP32-like apoptotic protease. J Biol Chem 271:25035-40 (1996). PubMed PMID: 8798786
Kirchgessner CU et al. DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect. Science 267:1178-83 (1995). PubMed PMID: 7855601
Poltoratsky VP et al. Human DNA-activated protein kinase (DNA-PK) is homologous to phosphatidylinositol kinases. J Immunol 155:4529-33 (1995). PubMed PMID: 7594449
Konno-Sato S et al. Phosphorylation of a 72-kDa nucleoprotein (NP-72) in HL-60 cells is mediated by the double-stranded DNA-dependent protein kinase (DNA-PK). Biochem Mol Biol Int 31:113-24 (1993). PubMed PMID: 8260934
Carter T et al. A DNA-activated protein kinase from HeLa cell nuclei. Mol Cell Biol 10:6460-71 (1990). PubMed PMID: 2247066

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DNA-PKcs / PRKDC / XRCC7 antibody

Western blot analysis of extracts from HeLa cells using DNA-PK antibody (#TA314389).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNA-PKcs / PRKDC / XRCC7 antibody

Western Blot: DNA PKcs Antibody (3H6) [TA336925] - Western blot analysis using DNA PKcs mAb against HEK293 (1) and DNA PKcs(AA: 2638-2971)-hIgGFc transfected HEK293 (2) cell lysate. Mouse IgG1 3H6 Purified Hu E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNA-PKcs / PRKDC / XRCC7 antibody

Western blot detection of DNA-PKcs in Hela and K562 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa,Observed band size:450KDa. Mouse IgG1 6D1-C11-F10 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNA-PKcs / PRKDC / XRCC7 antibody

Western blot detection of DNA-PKcs in Hela,K562,HL-60 and MOLT-4 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa,Observed band size:450KDa. Mouse 8D3-B12-F11 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNA-PKcs / PRKDC / XRCC7 antibody

Immunohistochemical analysis of paraffin-embedded breast cancer (left) and colon cancer (right) using PRKDC antibody Cat.-No AM06582SU-N with DAB staining. Mouse IgG1 3H6 Ascites Hu E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

DNA-PKcs / PRKDC / XRCC7 antibody

Western Blot and  Immunoprecipitation: DNA-PKcs Antibodies SuperNovus Pack [NB100-949] - Detection of Human DNA-PKcs on HeLa whole cell lysate using NB100-657. DNA-PKcs antibodies NB100-658, NB100-659 and NB100-660. Rabbit Aff - Purified Hu IP, WB
4 vials / €930.00
  Novus Biologicals Inc.

DNA-PKcs / PRKDC / XRCC7 pThr2609 antibody

WB using Anti-DNAPKcs antibody shows detection of a 460 kDa band corresponding to human DNAPKcs in various preparations. Lane 1: Fus1 untreated, Lane 2: Fus1 IR (20Gy, 4h), Lane 3: Fus1 DNAPK inhibitor + IR, Lane 4: MO59J (DNAPK-) untreated, Lane 5: MO59J IR, Lane 6: Fus1 untreated, Lane 7: Fus1 IR (20Gy, 4h), Lane 8: Fus1 DNAPK inhibitor + IR, Lane 9: MO59J untreated, Lane 10: MO59J IR.The primarey antibody was used at 1:1,000. Rabbit IgG Aff - Purified Can, Chimp, Chk, Eq, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNA-PKcs / PRKDC / XRCC7 antibody

Predicted band size: 469 kDa . Positive control: HT29 and HepG2 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: HT29 cells lysate Lane2: HepG2 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNA-PKcs / PRKDC / XRCC7 antibody

Hela cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-DNA-PKcs antibody (clone 7A4). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. Mouse IgG1 7A4 Purified Hu ICC/IF, IP, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNA-PKcs / PRKDC / XRCC7 antibody

MOLT4 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-DNA-PKcs (Thr2609) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Lane 1, untreated MOLT4 cells. Lane 2, MOLT4 cells exposed to 2 Gy radiation. Lane 3, MOLT4 cells exposed to 10 Gy radiation. In both cases, cells were harvested 30 minutes after radiation. Mouse IgG1 10B1 Purified Hu ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNA-PKcs / PRKDC / XRCC7 antibody

Western blot analysis on HeLa cell lysate using DNA-PK Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNA-PKcs / PRKDC / XRCC7 antibody

Western blot analysis of DNA PK expression in K562 cells Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNA-PKcs / PRKDC / XRCC7 antibody

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 2A8 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

DNA-PKcs / PRKDC / XRCC7 antibody

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG 1B9 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

DNA-PKcs / PRKDC / XRCC7 antibody

Hela cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-DNA-PKcs antibody (clone 7A4). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. Mouse IgG1 7A4 Purified Hu ICC/IF, IP, P
25 µg / €210.00
  Acris Antibodies GmbH

DNA-PKcs / PRKDC / XRCC7 antibody

DNA-PKcs / PRKDC / XRCC7 Rabbit Aff - Purified Hst, Hu, Ms, Rt WB
0.1 mg / €390.00
  Acris Antibodies GmbH

DNA-PKcs / PRKDC / XRCC7 pThr2609 antibody

Figure 1. Western blot analysis of extract from K562 cells untreated and treated with hydroxyurea, using DNA PKcs antibody (Lane 1 and 2) and DNA PKcs (phospho-Thr2609) antibody AP02448PU (Lane 3 and 4). Rabbit Aff - Purified Hu WB
0.1 ml / €320.00
  Acris Antibodies GmbH

DNA-PKcs / PRKDC / XRCC7 pThr2609 antibody

Figure 1. Western blot analysis of extract from K562 cells untreated and treated with hydroxyurea, using DNA PKcs antibody (Lane 1 and 2) and DNA PKcs (phospho-Thr2609) antibody AP02448PU (Lane 3 and 4). Rabbit Aff - Purified Hu WB
50 µl / €240.00
  Acris Antibodies GmbH

DNA-PKcs / PRKDC / XRCC7 antibody

Western blot analysis of extract from HT29 and HepG2 cells using DNA PKcs antibody Cat.-No AP02696PU-N/PU-S Rabbit Aff - Purified Hu WB
0.1 ml / €270.00
  Acris Antibodies GmbH

DNA-PKcs / PRKDC / XRCC7 antibody

Western blot analysis of extract from HT29 and HepG2 cells using DNA PKcs antibody Cat.-No AP02696PU-N/PU-S Rabbit Aff - Purified Hu WB
50 µl / €230.00
  Acris Antibodies GmbH

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