DNA polymerase beta antibody

Principal name

DNA polymerase beta antibody

Alternative names for DNA polymerase beta antibody

POLB

SwissProt ID

O57383 (Xenla), P06746 (Human), P06766 (Rat), Q27958 (Bovin), Q8K409 (Mouse)

Gene ID

5423 (POLB), 29240 (Polb)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Xen (Xenopus), Hst (Hamster), Chk (Chicken), Can (Canine), Eq (Equine), GP (Guinea Pig), Gt (Goat), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP), Frozen Sections (C), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of DNA polymerase beta antibody

DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases (1). In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches (2). These exonucleases include the family of DNA polymerases (3). DNA pol α, β, ∂, and e are involved in DNA replication and repair (4). DNA pol ∂ and DNA pol e are multisubunit enzymes, with DNA pol ∂ consisting of two subunits p125, which interacts with the sliding DNA clamp protein PCNA, and p50 (5). The nuclear-encoded DNA pol © is the only DNA polymerase required for the replication of the mitochondrial DNA (6). DNA pol Ω is ubiquitously expressed in various tissues and mediates the cellular mechanism of damage-induced mutagenesis (7). DNA pol œ is a DNA polymerase-helicase that binds ATP and is involved in the repair of interstrand crosslinks (8).

General readings

General / background references:Srivastava DK et al. Phorbol ester abrogates up-regulation of DNA polymerase beta by DNA-alkylating agents in Chinese hamster ovary cells. J Biol Chem 270:16402-8 (1995). PubMed PMID: 7608211

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DNA polymerase beta antibody

Western blot analysis of extracts of K562 cell lines, using POLB antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNA polymerase beta antibody

Western blot analysis of extracts from NIH/3T3 cells, using DNA Polymerase ß antibody (#TA311651).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNA polymerase beta antibody

Western Blot of Rabbit Anti-POLß (DNA polymerase beta) Antibody (TA319428). Lane 1: LN428 FLAG POLß (control). Lane 2: A172. Load: 35 µg per lane. Primary antibody: POLß antibody at 1:2000 for overnight at 4°C. Secondary antibody: IRDye800™ goat anti-rabbit at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~40 kDa, ~40 kDa. Other band(s): unknown band ~75 kDd. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNA polymerase beta antibody

Western blot analysis on NIH-3T3 cell lysate using DNA Polymerase beta Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNA polymerase beta antibody

WB Suggested Anti-POLB Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HT1080 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

DNA polymerase beta antibody

Immunohistochemical analysis in paraffin-embedded human breast carcinoma tissue using DNA polymerase beta Antibody Cat.-No AP06087PU-N. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

DNA polymerase beta antibody

Figure 1. Formalin fixed paraffin embedded human testis stained with DNA polymerase beta antibody DM319. Mouse IgG2a 61 Purified Hu P, WB
1 ml / €750.00
  Acris Antibodies GmbH

DNA polymerase beta antibody

Figure 1. Formalin fixed paraffin embedded human testis stained with DNA polymerase beta antibody DM319. Mouse IgG2a 61 Purified Hu P, WB
0.5 ml / €490.00
  Acris Antibodies GmbH

DNA polymerase beta antibody

POLB polyclonal antibody (A01), Lot # 050912JC01 Western Blot analysis of POLB expression in MES-SA/Dx5 ( Cat # L021V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

DNA polymerase beta antibody

Immunoperoxidase of purified MaxPab antibody to POLB on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml] Mouse Purified Hu E(detection), ICC/IF, P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

DNA polymerase beta antibody

Immunoprecipitation of POLB transfected lysate using anti-POLB MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with POLB MaxPab mouse polyclonal antibody (B01) (H00005423-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

DNA polymerase beta antibody

Immunoprecipitation/Western blot analysis of Polb expression in HeLa and MAD109 cells. Immunoprecipitation ( denatured verified ) using 2 µl/mg protein lysate. Use Protein G. Mouse IgG1 18S Bov, Hst, Hu, Ms, Rt IP
0.1 mg / €820.00
  Abnova Taiwan Corp.

DNA polymerase beta antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using DNA Polymerase beta Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

DNA polymerase beta antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using DNA Polymerase beta Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

DNA polymerase beta antibody

Human HT1080; WB Suggested Anti-POLB Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HT1080 cell lysate; POLB antibody - middle region (ARP56406_P050) in Human HT1080 cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

DNA polymerase beta antibody

Immunohistochemical analysis of DNA Polymerase beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

DNA polymerase beta antibody

Immunohistochemical analysis of DNA Polymerase beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

DNA polymerase beta antibody

Immunohistochemical analysis of DNA Polymerase beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

DNA polymerase beta antibody

DNA polymerase beta Mouse Serum Hu E, WB
50 µl / €420.00
  Novus Biologicals Inc.

DNA polymerase beta antibody

Immunohistochemistry: DNA Polymerase beta/DPOLB Antibody [NB100-91734] -  DNA Polymerase Beta/DPOLB antibody (Q324) [NB100-91734] - in paraffin embedded human breast carcinoma tissue. Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

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