DNA polymerase kappa antibody

Principal name

DNA polymerase kappa antibody

Alternative names for DNA polymerase kappa antibody

POLK, DINB1, DINB protein, DINP

SwissProt ID

Q96Q87 (Human), Q9QUG2 (Mouse), Q9UBT6 (Human)

Gene ID

51426 (POLK), 27015 (Polk)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Chimp (Chimpanzee), African clawed frog, Orang-Utan, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair)

Background of DNA polymerase kappa antibody

External and internal DNA-damaging agents continually threaten the integrity of genetic material in cells. Although a variety of repair mechanisms exist to remove the resulting lesions, some lesions escape repair and block the replication machinery. Members of the Y family of DNA polymerases, such as POLK, permit the continuity of the replication fork by allowing replication through such DNA lesions. Each Y family polymerase has a unique DNA-damage bypass and fidelity profile. POLK is specialized for the extension step of lesion bypass (summary by Lone et al., 2007 [PubMed 17317631]).[supplied by OMIM, Jan 2010]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF163570.1 [ECO:0000332] RNAseq introns :: single sample supports all introns ERS025081, ERS025084 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

General readings

Hile,S.E. (2008) Nucleic Acids Res. 36 (2), 688-696

  1. Quick-Filters:
  2. Highly Validated (1)

19 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DNA polymerase kappa antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 6F2 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

DNA polymerase kappa (817-830) antibody

  Goat polyclonal anti-POLK antibody  
WB using Anti-POLK antibody shows detection of a band ~98 kDa corresponding to human POLK (arrowhead).  Approximately 35 µg of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4? followed by washes and reaction with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] for 45 min at room temperature. Goat IgG Aff - Purified Chimp, Hu, Orang-Utan E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

DNA polymerase kappa antibody

  Rabbit Polyclonal Anti-POLK Antibody  
WB Suggested Anti-POLK Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: Human kidney Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

DNA polymerase kappa antibody

  Rabbit Polyclonal Anti-POLK Antibody  
WB Suggested Anti-POLK Antibody Titration: 0.2-1 ug/ml; Positive Control: 721_B cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

DNA polymerase kappa antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Prostate. Goat Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

POLK - human gene knockout kit via CRISPR

POLK - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

POLK - human gene knockout kit via CRISPR

POLK - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

POLK - human gene knockout kit via CRISPR

POLK - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

POLK - human gene knockout kit via CRISPR

POLK - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Polk - mouse gene knockout kit via CRISPR

Polk - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Polk - mouse gene knockout kit via CRISPR

Polk - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Polk - mouse gene knockout kit via CRISPR

Polk - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Polk - mouse gene knockout kit via CRISPR

Polk - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DNA polymerase kappa

  Recombinant protein of human polymerase (DNA directed) kappa (POLK)  
DNA polymerase kappa Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates (Knockout)

Catalog No.    

POLK knockout Cell lysate

POLK knockout Cell lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, POLK (mGFP-tagged) - Human polymerase (DNA directed) kappa (POLK), 200 uL, >10^7 TU/mL

Lenti ORF particles, POLK (mGFP-tagged) - Human polymerase (DNA directed) kappa (POLK), 200 uL, >10^7 TU/mL  
200 µl / €1,840.00
  OriGene Technologies, Inc.

Lenti ORF particles, POLK (Myc-DDK tagged) - Human polymerase (DNA directed) kappa (POLK) , 200 uL, >10^7 TU/mL

Lenti ORF particles, POLK (Myc-DDK tagged) - Human polymerase (DNA directed) kappa (POLK) , 200 uL, >10^7 TU/mL  
200 µl / €1,660.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Polk (GFP-tagged) - Mouse polymerase (DNA directed), kappa (Polk), 200 uL, >10^7 TU/mL

Lenti ORF particles, Polk (GFP-tagged) - Mouse polymerase (DNA directed), kappa (Polk), 200 uL, >10^7 TU/mL  
200 µl / €1,580.00
  OriGene Technologies, Inc.

Lenti ORF particles, Polk (Myc-DDK-tagged) - Mouse polymerase (DNA directed), kappa (Polk), 200 uL, >10^7 TU/mL

Lenti ORF particles, Polk (Myc-DDK-tagged) - Mouse polymerase (DNA directed), kappa (Polk), 200 uL, >10^7 TU/mL  
200 µl / €1,420.00
  OriGene Technologies, Inc.

19 Item(s)

per page
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