DNA polymerase kappa antibody

Principal name

DNA polymerase kappa antibody

Alternative names for DNA polymerase kappa antibody

POLK, DINB1, DINB protein, DINP

SwissProt ID

Q96Q87 (Human), Q9QUG2 (Mouse), Q9UBT6 (Human)

Gene ID

51426 (POLK)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Chimp (Chimpanzee), Orang-Utan, African clawed frog

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), EPair for Elisa (EPair)

Background of DNA polymerase kappa antibody

POLK (also known as DNA polymerase kappa, DINB protein and DINP) is involved in DNA repair. POLK plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. POLK lacks 3'-5' proofreading exonuclease activity. POLK is a nuclear protein that binds to REV1L and PCNA. POLK is detected throughout the nucleus and at replication foci. Multiple alternative splice variants have been detected for this protein. POLK is found at low levels in testis, spleen, prostate and ovary and at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.

General readings

Hile,S.E. (2008) Nucleic Acids Res. 36 (2), 688-696

  1. Quick-Filters:
  2. Highly Validated (2)

Items 1 to 20 of 23 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DNA polymerase kappa antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 6F2 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

DNA polymerase kappa antibody

Immunoperoxidase of monoclonal antibody to POLK on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml] Mouse IgG1 6F2 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DNA polymerase kappa (817-830) antibody

WB using Anti-POLK antibody shows detection of a band ~98 kDa corresponding to human POLK (arrowhead).  Approximately 35 µg of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4? followed by washes and reaction with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] for 45 min at room temperature. Goat IgG Aff - Purified Chimp, Hu, Orang-Utan E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNA polymerase kappa antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Prostate. Goat Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

DNA polymerase kappa antibody

DNA polymerase kappa Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

DNA polymerase kappa antibody

Immunofluorescence of purified MaxPab antibody to POLK on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

DNA polymerase kappa antibody

DNA polymerase kappa Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

DNA polymerase kappa antibody

Western Blot analysis of POLK expression in transfected 293T cell line (H00051426-T02) by POLK MaxPab polyclonal antibody.

Lane 1: POLK transfected lysate(54.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

DNA polymerase kappa antibody

DNA polymerase kappa Mouse IgG1 4F12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DNA polymerase kappa antibody

Western blot using POLK polyclonal antibody ( Cat # PAB11246 ) shows detection ofa band ~98 kDa corresponding to human POLK ( arrowhead ).
Approximately 35 µg of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed withthe primary antibody diluted to 1 : 1,000.
Reaction occurred overnight at 4°C followed bywashes and reaction with a 1 : 20,000 dilution of IRDyeTM800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at roomtemperature.
IRDyeTM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDyeis a trademark of LI-COR, Inc. Goat Chimp, Hu, Ms E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

DNA polymerase kappa antibody

Human kidney; WB Suggested Anti-POLK Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human kidney; POLK antibody - N-terminal region (ARP48792_P050) in Human kidney cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

DNA polymerase kappa antibody

Human 721_B; WB Suggested Anti-POLK Antibody Titration: 0.2-1 ug/ml. Positive Control: 721_B cell lysate; POLK antibody - middle region (ARP48793_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

DNA polymerase kappa antibody

Western Blot and Immunoprecipitation: Pol kappa Antibody [NBP1-06557] - Whole cell lysate from HeLa and 293T cells. Pol kappa was also immunoprecipitated by rabbit anti-Pol kappa antibodies NBP1-06558 and NBP1-06558. Rabbit IgG Aff - Purified Hu WB
0.1 ml / €440.00
  Novus Biologicals Inc.

DNA polymerase kappa antibody

Western Blot: Pol kappa Antibody [NBP1-06558] - Western Blot of Immunoprecipitates: Pol kappa Antibody[NBP1-06558]-Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Pol kappa antibody used for IP at 3 ug/mg lysate. Pol kappa was also immunoprecipitated by rabbit anti-Pol kappa antibodies NBP1-06557 and NBP1-06559, which recognize other epitopes. Rabbit IgG Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

DNA polymerase kappa antibody

Western Blot and Immunoprecipitation: Pol kappa Antibody [NBP1-06559] - Whole cell lysate from HeLa and 293T cells. Pol kappa was also immunoprecipitated by rabbit anti-Pol kappa antibodies NBP1-06557 and NBP1-06558. Rabbit IgG Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

DNA polymerase kappa antibody

Western Blot: DNA Polymerase Kappa Antibody [NBP1-58098] - This Anti-POLK antibody was used in Western Blot of Fetal Kidney   tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

DNA polymerase kappa antibody

Western Blot: DNA Polymerase Kappa Antibody [NBP1-58100] - This Anti-POLK antibody was used in Western Blot of 721_B tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DNA polymerase kappa

DNA polymerase kappa Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

DNA polymerase kappa

DNA polymerase kappa Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

DNA polymerase kappa

DNA polymerase kappa Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Items 1 to 20 of 23 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn