DNAJB1 / HSP40 antibody

Principal name

DNAJB1 / HSP40 antibody

Alternative names for DNAJB1 / HSP40 antibody

Heat shock 40 kDa protein 1, HSP-40, DnaJ protein homolog 1, HDJ-1, DNAJ1, HDJ1, HSPF1, DnaJ homolog subfamily B member 1

SwissProt ID

P25685 (Human), Q3MI00 (Bovin), Q9QYJ3 (Mouse)

Gene ID

3337 (DNAJB1), 3337889 (Q5L6N0)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Ye (Yeast), Bov (Bovine), Can (Canine), Chk (Chicken), Fi (Fish), GP (Guinea Pig), Hst (Hamster), Mky (Monkey), Por (Porcine), Rb (Rabbit), Sh (Sheep), Xen (Xenopus)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of DNAJB1 / HSP40 antibody

Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human Hsp40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of Hsp70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8).

General readings

Hata M et al.,(1996) Genomics 38(3):446?449
Manishkumar et al.,(2005) J Biol Chem 280(48):40041?40050
Christine M et al.,(2007) Genetics 175(4):1649?1664

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DNAJB1 / HSP40 antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-DNAJB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502195) Mouse IgG2b OTI1F9 Purified Can, Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DNAJB1 / HSP40 antibody

Western blot analysis of extracts of various cell lines, using DNAJB1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNAJB1 / HSP40 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DNAJB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502194) Mouse IgG2a OTI1E9 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DNAJB1 / HSP40 antibody

Anti-DNAJB1 mouse monoclonal antibody (TA502211) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DNAJB1(RC201762). Mouse IgG1 OTI1A12 Purified Hu, Mky F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DNAJB1 / HSP40 antibody

Anti-DNAJB1 mouse monoclonal antibody (TA502244) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DNAJB1(RC201762). Mouse IgG2a OTI1G4 Purified Can, Hu, Mky F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

DNAJB1 / HSP40 antibody

Western blot analysis of extracts from COLO205 cells, using HSP40 antibody (#TA312301).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNAJB1 / HSP40 antibody

Hsp40 HDJ1 visulaized using the antibody Mouse IgG1 3B9.E6 Aff - Purified Hu, Ms, Rt C, E, ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNAJB1 / HSP40 antibody

Western blot analysis of Hsp40 in HeLa cell lysates using a 1:1000 dilution of the antibody Rabbit Serum Hu, Mky, Ms, Rt IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNAJB1 / HSP40 antibody

Western blot analysis on COLO205 cell lysate using HSP40 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNAJB1 / HSP40 antibody

Anti-DNAJB1 mouse monoclonal antibody (TA502245) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DNAJB1(RC201762). Mouse IgG1 OTI1B12 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DNAJB1 / HSP40 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJB1 (RC201762, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJB1. Mouse IgG1 OTI1E10 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DNAJB1 / HSP40 antibody

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DNAJB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200) Mouse IgG2b OTI1H8 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DNAJB1 / HSP40 antibody

Western blot analysis on Jurkat cells: Cell lysates of Jurkat (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hsp40 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b k1C7 Purified Hu E, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

DNAJB1 / HSP40 (1-340) antibody

Western blot analysis on Jurkat cells: Cell lysates of Jurkat (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hsp40 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse k1C7 Purified Hu E, F, WB
50 µl / €220.00
  Acris Antibodies GmbH

DNAJB1 / HSP40 antibody

Hsp40 HDJ1 visulaized using the antibody Mouse IgG1 3B9.E6 Aff - Purified Hu, Ms, Rt C, E, ICC/IF, IP, WB
25 µg / €230.00
  Acris Antibodies GmbH

DNAJB1 / HSP40 (1-70) antibody

Western blot analysis of hsp40 expression in Jurkat (1), Raji (2), HeLa (3), MRC-5 (4), ZR-75-1 (5), NIH/3T3 (6), WR19L (7), P19 (8) and PC12 (9) using AM26593AF-N. Mouse IgG2a 2E1 Azide Free Hu, Ms IP, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

DNAJB1 / HSP40 antibody

Immunohistochemistry analysis of DNAJB1 / HSP40 Antibody (Cat.-No AP06173PU-N) in paraffin-embedded lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

DNAJB1 / HSP40 antibody

IHC Image for AP22890PU-N
Spleen, neutrophils, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Bov, Can, Chk, Fi, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh, Xen IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

DNAJB1 / HSP40 (C-term) antibody

Immunohistochemical analysis of paraffin embedded rat tissue sections (brain), staining NGFbeta in cytoplasm DAB chromogenic reaction Rabbit IgG Aff - Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

DNAJB1 / HSP40 antibody

DNAJB1 MaxPab polyclonal antibody. Western Blot analysis of DNAJB1 expression in human pancreas. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

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