DNMT1 antibody

Principal name

DNMT1 antibody

Alternative names for DNMT1 antibody

MCMT, HsaI, AIM, CXXC9, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase HsaI, CXXC-type zinc finger protein 9

SwissProt ID

P13864 (Mouse), P26358 (Human), Q24K09 (Bovin), Q92072 (Chick), Q9Z330 (Rat)

Gene ID

1786 (DNMT1), 13433 (Dnmt1)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Por (Porcine), Rt (Rat), Ze (Zebrafish), Xen (Xenopus)

Available hosts

Rabbit, Mouse, Goat, Chicken, Rat

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), Enzyme Immunoassay (E), Paraffin Sections (P), Dot blot (Dot), ChIP assay (ChIP), Flow Cytometry (F), Immunofluorescence (IF)

Background of DNMT1 antibody

Methylation of D at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting and development (1,2). Three families of mammalian D methyltransferases have been identified: DNMT1, DNMT2 and DNMT3 (1,2). DNMT1 is constitutively expressed in proliferating cells and functions as a maintence methyltransferase, transferring proper methylation patterns to newly synthesized D during replication. DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues. DNMT3A and DNMT3B function as de novo methyltransferases that methylate previously unmethylated regions of D. DNMT2 is expressed at low levels in adult somatic tissues and its ictivation affects neither de novo nor maintence D methylation. DNMT1, DNMT3A and DNMT3B together form a protein complex that interacts with histone deacetylases (HDAC1, HDAC2, Sin3A), transcriptiol repressor proteins (RB, TAZ-1) and heterochromatin proteins (HP1, SUV39H1), to maintain proper levels of D methylation and facilitate gene silencing (3-8). Improper D methylation contributes to diseased states such as cancer (1,2). Hypermethylation of promoter CpG islands within tumor suppressor genes correlates with gene silencing and the development of cancer. In addition, hypomethylation of bulk genomic D correlates with and may contribute to the onset of cancer. DNMT1, DNMT3A and DNMT3B are over-expressed in many cancers, including acute and chronic myelogenous leukemias, in addition to colon, breast and stomach carcinomas (9-12).

General readings

Dion V, Lin Y, Hubert L Jr, Waterland RA, Wilson JH. Dnmt1 deficiency promotes CAG repeat expansion in the mouse germline. Hum Mol Genet. 2008 May 1;17(9):1306-17. Epub 2008 Feb 5. PMID: 18252747

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DNMT1 antibody

Western Blot: Dnmt1 Antibody [TA336293] - Analysis using TA336293 to detect DNMT1 levels in T24 degrees Cells. Lane 1: 50 ugs of total protein lysate from untreated T24 degrees Cells. Lane 2: 50 ugs of total protein lysate treated with 0.5 uM 5-Aza-Deoxyc Rabbit Purified Hu, Ms C, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNMT1 antibody

TA303330 (0.5µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, P, WB
20 µg / €200.00
  Acris Antibodies GmbH

DNMT1 antibody

TA303330 (0.5µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNMT1 antibody

Predicted cell location: Cytoplasm, Nucleus . Positive control: Human cervical cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using DNMT1 antibody at dilution 1/35, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNMT1 antibody

Predicted cell location: Cytoplasm, Nucleus . Positive control: Human cervical cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using DNMT1 antibody at dilution 1/12, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNMT1 (637-650) antibody

Western blot analysis of DNMT1 in H1299 lysate using a 1:1000 dilution of the antibody Mouse IgG1 60B1220.1 Purified Hu, Ms, Ze ChIP, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNMT1 antibody

Western blot analysis of DNMT1 in H1299 lysates using a 1:1000 dilution of the antibody Mouse IgG1 4G11-C7 Purified Hu, Ms, Ze C, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNMT1 antibody

Western blot analysis of extracts of various cell lines, using DNMT1 antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNMT1 antibody

Western Blot: Dnmt1 Antibody (60B1220.1) [TA336382] - analysis of Dnmt1 in 2102EP (human embryonic carcinoma) in the A) absence and B) presence of immunizing peptide, recombinant human Dnmt1 protein in the C) absence and D) presence of immunizing peptide, Mouse IgG1 60B1220.1 Purified Hu, Ms, Ze C, ChIP, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNMT1 antibody

Western Blot: Dnmt1 Antibody [TA336890] - analysis of DNMT1 in Jurkat cell lysate (35ug protein in RIPA buffer) using this antibody. 5ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNMT1 antibody

WB Suggested Anti-Dnmt1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Mouse Lung Rabbit IgG Purified Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

DNMT1 antibody

WB was performed on nuclear extracts from N2A cells (N2A NE, 60 ug) with the antibody against mouse DNMT1, diluted 1:500 in TBS-Tween containing 5% skimmed milk is shown on the left; the location of the protein of interest is indicated on the right. Rabbit IgG Purified Ms E
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNMT1 antibody

WB was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse DNMT1, diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 183 kDa) is indicated on the right. Rabbit IgG Purified Ms E
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNMT1 (1603-1616) antibody

Figure 1. Staining DNMT1 in Testis by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Purified Hu E, P
50 µg / €470.00
  Acris Antibodies GmbH

DNMT1 (177-550) antibody

Placenta: Formalin-Fixed Paraffin-Embedded (FFPE) Chicken Ig Azide Free Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

DNMT1 (C-term) antibody

Western blot analysis using Polyclonal anti-Dnmt1 antibody (C-term) (Cat.#AP11047PU-N) to detect Dnmt1 in Jurkat cell lysate. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DNMT1 pSer714 antibody

Dot blot analysis of anti-Dnmt1 Phospho-specific antibody (Cat.#AP12931PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

DNMT1 pSer154 antibody

Dot blot analysis of anti-Phospho-Dnmt1 pSer154 Antibody (Cat.#AP12946PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

DNMT1 antibody

Western Blot analysis of Human DNMT1 expression in nuclear extracts of HeLa cells (50 µg/lane). SP2063P DNMT1 antibody was used at the indicated concentrations. Rabbit Aff - Purified Hu IP, WB
50 µg / €330.00
  Acris Antibodies GmbH

DNMT1 antibody

IHC analysis of DNMT1. Using DNMT1 monoclonal antibody, clone 60B1220.1 ( Cat # MAB0079 ) at 2 µg/ml on formalin-fixed, paraffin-embedded medullar kidney tissue sections. Mouse IgG1 60B1220.1 Hu, Ms ChIP, P, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

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