DNMT2 / TRDMT1 antibody

Principal name

DNMT2 / TRDMT1 antibody

Alternative names for DNMT2 / TRDMT1 antibody

HsaIIP, PuMet, tRNA (cytosine-5-)-methyltransferase, DNA (cytosine-5)-methyltransferase-like protein 2, DNA methyltransferase homolog HsaIIP

SwissProt ID

O14717 (Human), O55055 (Mouse), Q4G073 (Rat), Q7YS61 (Bovin)

Gene ID

13434 (Trdmt1), 1787 (TRDMT1)

Available reactivities

Ms (Mouse), Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Rt (Rat), African clawed frog

Available hosts

Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of DNMT2 / TRDMT1 antibody

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2Fbinding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.

General readings

General / background references:Yoder JA & Bestor TH A candidate mammalian DNA methyltransferase related to pmt1p of fission yeast. Hum Mol Genet 7:279-84 (1998). PubMed PMID: 9425235
Okano M et al. Dnmt2 is not required for de novo and maintenance methylation of viral DNA in embryonic stem cells. Nucleic Acids Res 26:2536-40 (1998). PubMed PMID: 9592134

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DNMT2 / TRDMT1 antibody

Western Blot: Dnmt2 Antibody (102B1259.2) [TA336596] - Analysis of Dnmt2 in transfected cell lysate using Dnmt2 monoclonal antibody at 2 ug/ml. Mouse IgG1 102B1259.2 Purified Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNMT2 / TRDMT1 antibody

Western Blot: Dnmt2 Antibody [TA336598] - analysis of Dnmt2 in mouse ES cell lysate using Dnmt2 antibodyat 2 ug/ml. Rabbit Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNMT2 / TRDMT1 antibody

WB was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Dnmt2, diluted 1:1000 in BSA/PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (47 kDa) is indicated on the right. Rabbit IgG Purified Ms E
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNMT2 / TRDMT1 antibody

Immunohistochemistry: AP11029PU-N Dnmt2 antibody staining of Formalin-Fixed, Paraffin-Embedded Human cancer tissue. The primary antibody was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DNMT2 / TRDMT1 (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DNMT2 / TRDMT1 antibody

Western Blot analysis of DNMT2 expression in transfected 293T cell line by DNMT2 MaxPab polyclonal antibody.

Lane 1: DNMT2 transfected lysate(43.01 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

DNMT2 / TRDMT1 antibody

TRDMT1 MaxPab rabbit polyclonal antibody. Western Blot analysis of TRDMT1 expression in MCF-7. Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

DNMT2 / TRDMT1 antibody

Western Blot analysis of TRDMT1 expression in transfected 293T cell line by TRDMT1 MaxPab rabbit polyclonal antibody.

Lane 1: TRDMT1 transfected lysate(44.60 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

DNMT2 / TRDMT1 antibody

Immunofluorescence of monoclonal antibody to DNMT2 on HeLa cell. [antibody concentration 40 ug/ml] Mouse IgG1 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DNMT2 / TRDMT1 antibody

Western blot analysis of Trdmt1 in mouse ES cell lysate using Trdmt1 polyclonal antibody ( Cat # PAB0161 ) at 2 µg/ml. Rabbit Hu, Ms WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

DNMT2 / TRDMT1 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

DNMT2 / TRDMT1 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the Trdmt1 polyclonal antibody ( Cat # PAB2228 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

DNMT2 / TRDMT1 antibody

DNMT2 / TRDMT1 Rabbit Hu, Ms ICC/IF, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

DNMT2 / TRDMT1 antibody

Western blot was performed on whole cell lysates from mouse fibroblasts ( Lane 1, NIH/3T3 ) and embryonic stem cells ( Lane 2, E14Tg2a ) with Trdmt1 polyclonal antibody ( Cat # PAB14062 ), diluted 1 : 1000 in BSA/PBS-Tween. Rabbit Ms E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

DNMT2 / TRDMT1 antibody

Human Muscle; WB Suggested Anti-TRDMT1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle; TRDMT1 antibody - N-terminal region (ARP48627_P050) in Human Muscle cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

DNMT2 / TRDMT1 antibody

Western Blot: Dnmt2 Antibody [NBP1-53132] - This Anti-TRDMT1 antibody was used in Western Blot of Fetal Muscle tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DNMT2 / TRDMT1 (1-391, His-tag)

DNMT2 / TRDMT1 Human Purified > 90 % by SDS - PAGE E. coli
0.25 mg / €940.00
  Acris Antibodies GmbH

DNMT2 / TRDMT1 (1-391, His-tag)

DNMT2 / TRDMT1 Human Purified > 90 % by SDS - PAGE E. coli
50 µg / €370.00
  Acris Antibodies GmbH

DNMT2 / TRDMT1

DNMT2 / TRDMT1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

DNMT2 / TRDMT1

DNMT2 / TRDMT1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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