DNMT3A antibody

Principal name

DNMT3A antibody

Alternative names for DNMT3A antibody

DNA MTase HsaIIIA, M.HsaIIIA, DNA (cytosine-5)-methyltransferase 3A, DNA methyltransferase HsaIIIA

SwissProt ID

O88508 (Mouse), Q1LZ53 (Rat), Q4W5Z4 (Chick), Q9Y6K1 (Human)

Gene ID

1788 (DNMT3A), 13435 (Dnmt3a)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Prim (Primate)

Available hosts

Rabbit, Mouse, Chicken

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C), Enzyme Immunoassay (E), ChIP assay (ChIP), ELISA (detection) (E(detection))

Background of DNMT3A antibody

Methylation of D at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting and development. Three families of mammalian D methyltransferases have been identified: DNMT1, DNMT2 and DNMT3. DNMT1 is constitutively expressed in proliferating cells and functions as a maintence methyltransferase, transferring proper methylation patterns to newly synthesized D during replication. DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues. DNMT3A and DNMT3B function as de novo methyltransferases that methylate previously unmethylated regions of D. DNMT2 is expressed at low levels in adult somatic tissues and its ictivation affects neither de novo nor maintence D methylation. DNMT1, DNMT3A and DNMT3B together form a protein complex that interacts with histone deacetylases (HDAC1, HDAC2, Sin3A), transcriptiol repressor proteins (RB, TAZ-1) and heterochromatin proteins (HP1, SUV39H1), to maintain proper levels of D methylation and facilitate gene silencing. Improper D methylation contributes to diseased states such as cancer. Hypermethylation of promoter CpG islands within tumor suppressor genes correlates with gene silencing and the development of cancer. In addition, hypomethylation of bulk genomic D correlates with and may contribute to the onset of cancer. DNMT1, DNMT3A and DNMT3B are over-expressed in many cancers, including acute and chronic myelogenous leukemias, in addition to colon, breast and stomach carcinomas.

General readings

This product has been used in:Chen T et al. A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation. J Biol Chem 277:38746-54 (2002). PubMed PMID: 12138111
Dennis K et al. Lsh, a member of the SNF2 family, is required for genome-wide methylation. Genes Dev 15:2940-4 (2001). PubMed PMID: 11711429
General / background references:Xie S et al. Cloning, expression and chromosome locations of the human DNMT3 gene family. Gene 236:87-95 (1999). PubMed PMID: 10433969
Okano M et al. DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell 99:247-57 (1999). PubMed PMID: 10555141
Reik W et al. Dissecting de novo methylation. Nat Genet 23:380-2 (1999). PubMed PMID: 10581015
Hansen RS et al. The DNMT3B DNA methyltransferase gene is mutated in the ICF immunodeficiency syndrome. Proc Natl Acad Sci U S A 96:14412-7 (1999). PubMed PMID: 10588719

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DNMT3A antibody

Western blot analysis to detect overexpressed DNMT3A level in HCT-116 cells. Lane 1: 40 ug of nuclear extract from untreated HCT-116 cells. Lane 2: 40 ug of Nuclear extract from HCT-116 cells overexpressing DNMT3A. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNMT3A antibody

Predicted band size: 78 kDa . Positive control: A549 cell lysate. Recommended dilution: 1/1000-5000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/1350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt P
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNMT3A antibody

Western blot analysis of Jurkat cell using TA322196(DNMT3A Antibody) at dilution 1/1350 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNMT3A antibody

Western blot analysis of extracts of various cell lines, using DNMT3A antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNMT3A antibody

Western Blot: Dnmt3a Antibody (64B814.1) [TA336383] - Analysis of Dnmt3a in (A) Dnmt3a transfected and (B) untransfected 293 cell lysate (Novus, 40212) using Dnmt3a antibody at 1 ug/ml. Mouse IgG1 64B814.1 Purified Hu, Ms ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNMT3A antibody

Western Blot: Dnmt3a Antibody (64B1446) [TA336580] - Analysis of (A) Dnmt3a transfected 293 cell lysate and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/ml. Mouse IgG1 64B1446 Purified Hu, Ms ChIP, F, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNMT3A antibody

Western Blot: Dnmt3a Antibody [TA336891] - analysis using Dnmt3a antibody at 5 ug/ml with Dnmt3a transfected 293 cell lysate . Rabbit Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNMT3A antibody

Western Blot: Dnmt3a Antibody [TA336977] - Analysis of Dnmt3a in Dnmt3a transfected 293 cell lysate using TA336977 at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Rabbit Purified Hu, Prim WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNMT3A antibody

WB analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and WB analysis was performed with the DNMT3A antibody (diluted 1:2,000) (lane 3). Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

DNMT3A antibody

WB analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and WB analysis was performed with the DNMT3A antibody (diluted 1:2,500) (lane 3). Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

DNMT3A antibody

WB analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and WB analysis was performed with the DNMT3A antibody (diluted 1:2,000) (lane 3). Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

DNMT3A antibody

C15310107-1,WB using the antibody against mouse DNMT3A diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Rabbit IgG Purified Hu, Ms E
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNMT3A (C-term) antibody

Figure 1. Western blot analysis of DNMT3A Antibody (C-term) (Cat.# AP11035PU-N) in HepG2 cell line lysates (35ug/lane). DNMT3A (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DNMT3A antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DNMT3A antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DNMT3A (N-term) antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Dnmt3a antibody (N-term) (Cat.#AP11051PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DNMT3A (Center) antibody

Western blot analysis of DNMT3A Antibody (Center) Cat.-No AP51302PU-N in HL-60 cell line lysates (35ug/lane).This demonstrates the DNMT3A antibody detected the DNMT3A protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DNMT3A (C-term) antibody

Western blot analysis of DNMT3A Antibody (C-term) Cat.-No AP51303PU-N in CEM cell line lysates (35ug/lane). This demonstrates the DNMT3A antibody detected the DNMT3A protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DNMT3A antibody

Immunofluorescence of purified MaxPab antibody to DNMT3A on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

DNMT3A antibody

Immunoprecipitation of DNMT3A transfected lysate using anti-DNMT3A MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with DNMT3A MaxPab mouse polyclonal antibody (B01) (H00001788-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

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