DNMT3A antibody

Principal name

DNMT3A antibody

Alternative names for DNMT3A antibody

DNA MTase HsaIIIA, M.HsaIIIA, DNA (cytosine-5)-methyltransferase 3A, DNA methyltransferase HsaIIIA

SwissProt ID

O88508 (Mouse), Q1LZ53 (Rat), Q4W5Z4 (Chick), Q9Y6K1 (Human)

Gene ID

1788 (DNMT3A), 13435 (Dnmt3a), 444984

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Prim (Primate), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ChIP assay (ChIP), Enzyme Immunoassay (E), Flow Cytometry (F)

Background of DNMT3A antibody

Methylation of D at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting and development. Three families of mammalian D methyltransferases have been identified: DNMT1, DNMT2 and DNMT3. DNMT1 is constitutively expressed in proliferating cells and functions as a maintence methyltransferase, transferring proper methylation patterns to newly synthesized D during replication. DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues. DNMT3A and DNMT3B function as de novo methyltransferases that methylate previously unmethylated regions of D. DNMT2 is expressed at low levels in adult somatic tissues and its ictivation affects neither de novo nor maintence D methylation. DNMT1, DNMT3A and DNMT3B together form a protein complex that interacts with histone deacetylases (HDAC1, HDAC2, Sin3A), transcriptiol repressor proteins (RB, TAZ-1) and heterochromatin proteins (HP1, SUV39H1), to maintain proper levels of D methylation and facilitate gene silencing. Improper D methylation contributes to diseased states such as cancer. Hypermethylation of promoter CpG islands within tumor suppressor genes correlates with gene silencing and the development of cancer. In addition, hypomethylation of bulk genomic D correlates with and may contribute to the onset of cancer. DNMT1, DNMT3A and DNMT3B are over-expressed in many cancers, including acute and chronic myelogenous leukemias, in addition to colon, breast and stomach carcinomas.

7 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DNMT3A antibody

  Anti-DNMT3A Rabbit Polyclonal Antibody  
Predicted band size: 78 kDa . Positive control: A549 cell lysate. Recommended dilution: 1/1000-5000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/1350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt P
0.1 ml / €335.00
  OriGene Technologies, Inc.

DNMT3A antibody

  Anti-DNMT3A Rabbit Polyclonal Antibody  
Western blot analysis of Jurkat cell using TA322196(DNMT3A Antibody) at dilution 1/1350 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

DNMT3A antibody

  Mouse Monoclonal DNMT3A Antibody (64B1446)  
Western Blot: Dnmt3a Antibody (64B1446) [TA336580] - Analysis of (A) Dnmt3a transfected 293 cell lysate and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/ml. Mouse IgG1 64B1446 Purified Hu, Ms ChIP, F, ICC/IF, P, WB
0.1 mg / €474.00
  OriGene Technologies, Inc.

DNMT3A antibody

  Rabbit Polyclonal Anti-DNMT3A Antibody  
DNMT3A Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.

DNMT3A antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  OriGene Technologies GmbH

DNMT3A antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  OriGene Technologies GmbH

DNMT3A (N-term) antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Dnmt3a antibody (N-term) (Cat.#AP11051PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  OriGene Technologies GmbH

7 Item(s)

per page
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