DNMT3B antibody

Principal name

DNMT3B antibody

Alternative names for DNMT3B antibody

DNA MTase HsaIIIB, M.HsaIIIB, DNA (cytosine-5)-methyltransferase 3B, DNA methyltransferase HsaIIIB

SwissProt ID

O88509 (Mouse), Q9UBC3 (Human), Q9UBC3-3

Gene ID

1789 (DNMT3B), 13436 (Dnmt3b)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Chk (Chicken), Por (Porcine), Rt (Rat), Ze (Zebrafish), Mky (Monkey), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ChIP assay (ChIP), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Frozen Sections (C), Flow Cytometry (F)

Background of DNMT3B antibody

DNMT3B is required for genome wide de novo methylation and is essential for the establishment of D methylation patterns during development. D methylation is coordited with methylation of histones. DNMT3B may preferentially methylate nucleosomal D within the nucleosome core region. DNMT3B may function as transcriptiol co-repressor by associating with CBX4 and independently of D methylation. DNMT3B seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, DNMT3B is involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome ictivation. Studies in mice have demonstrated that D methylation is required for mammalian development. This gene encodes a D methyltransferase which is thought to function in de novo methylation, rather than maintence methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six altertively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DNMT3B antibody

  Rabbit polyclonal anti-DNMT3B antibody  
Western blot analysis of extracts from HT-29 cells, using DNMT3B antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNMT3B antibody

  DNMT3B Rabbit Polyclonal Antibody  
Western blot analysis of extracts of A549 cell line, using DNMT3B antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNMT3B antibody

  Rabbit polyclonal Anti-DNMT3B Antibody  
Sample Type : Lane 1: 20ug mouse mesenchymal stem cell lysate Primary Antibody Dilution : 1:2000 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1:10,000 Color/Signal Descriptions: DNMT3B Gene Name: Anonymous Submitted by: Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

DNMT3B antibody

  Rabbit Polyclonal DNMT3B Antibody  
Western Blot: Dnmt3b Antibody [TA336294] - Analysis using TA336294 to detect overexpressed DNMT-38 in HCT116 cells. Lane 1: 40 ug of nuclear extract from untransfected T24 degrees Cells. Lane 2-3: 40 ug of nuclear extracts transiently transfected to overe Rabbit Purified Hu, Ms C, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNMT3B antibody

  Mouse Monoclonal DNMT3B Antibody (52A1018)  
Western Blot: Dnmt3b Antibody (52A1018) [TA336379] - Analysis for Dnmt3b  at 2 ug/ml against 10 ug of cell lysates of 293 transfected with mouse Dnmt3a (Lanes 1) and Dnmt3b (Lanes 2). A protein band of approximate mol. wt. of 110 kDa is only detected in 2 Mouse IgG1 52A1018 Purified Hu, Ms C, ChIP, F, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNMT3B antibody

  Rabbit Polyclonal DNMT3B Antibody  
WB using the antibody against DNMT3B diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

DNMT3B antibody

  Rabbit Polyclonal DNMT3B Antibody  
WB using the antibody against DNMT3B diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the MW marker (in kDa) is shown on the left. Rabbit IgG Purified Hu, Ms E
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNMT3B antibody

Immunohistochemistry (IHC) analyses of Dnmt3b antibody (Cat.-No. AP01182PU-N) in Paraffin-Embedded Human liver carcinoma tissue. Rabbit Aff - Purified Hu P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

DNMT3B antibody

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Dnmt3b antibody (Cat.#AP11052PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DNMT3B (1-50) antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu E, ICC/IF, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

DNMT3B antibody

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using DNMT3B Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €230.00
  Assay Biotech

DNMT3B antibody

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using DNMT3B Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

DNMT3B - human gene knockout kit via CRISPR

DNMT3B - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

DNMT3B - human gene knockout kit via CRISPR

DNMT3B - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

DNMT3B - human gene knockout kit via CRISPR

DNMT3B - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

DNMT3B - human gene knockout kit via CRISPR

DNMT3B - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dnmt3b - mouse gene knockout kit via CRISPR

Dnmt3b - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Dnmt3b - mouse gene knockout kit via CRISPR

Dnmt3b - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dnmt3b - mouse gene knockout kit via CRISPR

Dnmt3b - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dnmt3b - mouse gene knockout kit via CRISPR

Dnmt3b - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

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