DNMT3B antibody

Principal name

DNMT3B antibody

Alternative names for DNMT3B antibody

DNA MTase HsaIIIB, M.HsaIIIB, DNA (cytosine-5)-methyltransferase 3B, DNA methyltransferase HsaIIIB

SwissProt ID

O88509 (Mouse), Q9UBC3 (Human), Q9UBC3-3

Gene ID

13436 (Dnmt3b), 1789 (DNMT3B)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), Mky (Monkey), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Chicken, Mouse, Rat

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), ChIP assay (ChIP), Enzyme Immunoassay (E), Immunofluorescence (IF), Immunohistochemistry (IHC)

Background of DNMT3B antibody

DNMT3B is required for genome wide de novo methylation and is essential for the establishment of D methylation patterns during development. D methylation is coordited with methylation of histones. DNMT3B may preferentially methylate nucleosomal D within the nucleosome core region. DNMT3B may function as transcriptiol co-repressor by associating with CBX4 and independently of D methylation. DNMT3B seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, DNMT3B is involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome ictivation. Studies in mice have demonstrated that D methylation is required for mammalian development. This gene encodes a D methyltransferase which is thought to function in de novo methylation, rather than maintence methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six altertively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.

General readings

Okano M et al. DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell 99:247-57 (1999). PubMed PMID: 10555141

  1. Quick-Filters:
  2. Highly Validated (1)

Items 1 to 20 of 36 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DNMT3B antibody

Western blot analysis of extracts from HT-29 cells, using DNMT3B antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNMT3B antibody

Western blot analysis of extracts of A549 cell line, using DNMT3B antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNMT3B antibody

Sample Type : Lane 1: 20ug mouse mesenchymal stem cell lysate Primary Antibody Dilution : 1:2000 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1:10,000 Color/Signal Descriptions: DNMT3B Gene Name: Anonymous Submitted by: Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

DNMT3B antibody

Western Blot: Dnmt3b Antibody [TA336294] - Analysis using TA336294 to detect overexpressed DNMT-38 in HCT116 cells. Lane 1: 40 ug of nuclear extract from untransfected T24 degrees Cells. Lane 2-3: 40 ug of nuclear extracts transiently transfected to overe Rabbit Purified Hu, Ms C, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNMT3B antibody

Western Blot: Dnmt3b Antibody (52A1018) [TA336379] - Analysis for Dnmt3b  at 2 ug/ml against 10 ug of cell lysates of 293 transfected with mouse Dnmt3a (Lanes 1) and Dnmt3b (Lanes 2). A protein band of approximate mol. wt. of 110 kDa is only detected in 2 Mouse IgG1 52A1018 Purified Hu, Ms C, ChIP, F, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNMT3B antibody

WB using the antibody against DNMT3B diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

DNMT3B antibody

WB using the antibody against DNMT3B diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the MW marker (in kDa) is shown on the left. Rabbit IgG Purified Hu, Ms E
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNMT3B antibody

Immunohistochemistry (IHC) analyses of Dnmt3b antibody (Cat.-No. AP01182PU-N) in Paraffin-Embedded Human liver carcinoma tissue. Rabbit Aff - Purified Hu P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

DNMT3B antibody

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Dnmt3b antibody (Cat.#AP11052PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DNMT3B (1-50) antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

DNMT3B antibody

DNMT3B Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

DNMT3B antibody

Western blot analysis for Dnmt3b using Dnmt3b monoclonal antibody, clone 52A1018 ( Cat # MAB0078 ) at 2 µg/ml against 10 µg of cell lysates of 293 transfected with mouse Dnmt3a (lane 1) and Dnmt3b (lane 2). A protein band of approximate 110 kDa is only detected in 293 cells transfected with mouse Dnmt3b cDNA. Mouse IgG1 52A1018 Hu, Ms ChIP, ICC/IF, IP, P, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

DNMT3B antibody

Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with DNMT3B polyclonal antibody ( Cat # PAB2207 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

DNMT3B antibody

DNMT3B Rabbit Hu, Ms IP, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

DNMT3B antibody

Nuclear extracts from HeLa cells ( 40 µg ) were analysed by Western blot using Dnmt3b polyclonal antibody ( Cat # PAB14046 ) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk. Rabbit Hu, Ms E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

DNMT3B antibody

ELISA was performed using a serial dilution of Dnmt3b polyclonal antibody ( Cat # PAB14125 ) and crude serum in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 900. Rabbit Hu, Ms ChIP, E
5 µg / €470.00
  Abnova Taiwan Corp.

DNMT3B antibody

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using DNMT3B Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

DNMT3B antibody

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using DNMT3B Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

DNMT3B antibody

mouse mesenchymal stem; . Sample Type :. Lane 1: 20ug mouse mesenchymal stem cell lysate . Primary Antibody Dilution :. 1:2000 . Secondary Antibody:. Anti-rabbit-HRP . Secondary Antibody Dilution:. 1:10,000 . Color/Signal Descriptions:. DNMT3B . Gene Name:. Anonymous . Submitted by:.; DNMT3B antibody - middle region (ARP49124_P050) in mouse mesenchymal stem cells using Western Blot Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

DNMT3B antibody

Immunohistochemical analysis of DNMT3B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Items 1 to 20 of 36 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn