DNMT3B antibody
Principal name
DNMT3B antibody
Alternative names for DNMT3B antibody
DNA MTase HsaIIIB, M.HsaIIIB, DNA (cytosine-5)-methyltransferase 3B, DNA methyltransferase HsaIIIB
Ncbi ID
9606, 10090, NP_008823.1, NP_008823, NP_787045, AAI18503.1, NM_175849, NM_006892, NP_001193984.1, NP_001003960.2, NM_001003961, NM_001003960, NM_010068, NM_001003963, NM_001122997, NM_175850
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ChIP assay (ChIP), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Frozen Sections (C), Flow Cytometry (F)
Background of DNMT3B antibody
DNMT3B is required for genome wide de novo methylation and is essential for the establishment of D methylation patterns during development. D methylation is coordited with methylation of histones. DNMT3B may preferentially methylate nucleosomal D within the nucleosome core region. DNMT3B may function as transcriptiol co-repressor by associating with CBX4 and independently of D methylation. DNMT3B seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, DNMT3B is involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome ictivation. Studies in mice have demonstrated that D methylation is required for mammalian development. This gene encodes a D methyltransferase which is thought to function in de novo methylation, rather than maintence methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six altertively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.
Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with DNMT3B polyclonal antibody ( Cat # PAB2207 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the DNMT3B polyclonal antibody ( Cat # PAB2207 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
Western blot analysis of DNMT3B polyclonal antibody ( Cat # PAB2207 ) in T-47D cell lysate. DNMT3B ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot analysis for Dnmt3b using Dnmt3b monoclonal antibody, clone 52A1018 ( Cat # MAB0078 ) at 2 µg/ml against 10 µg of cell lysates of 293 transfected with mouse Dnmt3a (lane 1) and Dnmt3b (lane 2). A protein band of approximate 110 kDa is only detected in 293 cells transfected with mouse Dnmt3b cDNA.
Western blot analysis of Dnmt3b in A) NIH/3T3 cell lysate and B) recombinant Dnmt3b1 protein using Dnmt3b monoclonal antibody, clone 52A1018 ( Cat # MAB0078 ) at 4 µg/ml and 0.5 µg/ml, respectively.
Immunohistochemistry (IHC) analyses of Dnmt3b antibody (Cat.-No. AP01182PU-N) in Paraffin-Embedded Human liver carcinoma tissue.
Western blot (WB) analysis of Dnmt3b antibody (Cat.-No. AP01182PU-N) in extracts from HT-29 cells.
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Dnmt3b antibody (Cat.#AP11052PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-Dnmt3b Pab (Cat.#AP11052PU-N) in T47-D cell lysate. Dnmt3b (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Nuclear extracts from HeLa cells ( 40 µg ) were analysed by Western blot using Dnmt3b polyclonal antibody ( Cat # PAB14046 ) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk.
ELISA was performed using a serial dilution of Dnmt3b polyclonal antibody ( Cat # PAB14046 ).By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 13,500.
ELISA was performed using a serial dilution of Dnmt3b polyclonal antibody ( Cat # PAB14125 ) and crude serum in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 900.
mouse mesenchymal stem; . Sample Type :. Lane 1: 20ug mouse mesenchymal stem cell lysate . Primary Antibody Dilution :. 1:2000 . Secondary Antibody:. Anti-rabbit-HRP . Secondary Antibody Dilution:. 1:10,000 . Color/Signal Descriptions:. DNMT3B . Gene Name:. Anonymous . Submitted by:.; DNMT3B antibody - middle region (ARP49124_P050) in mouse mesenchymal stem cells using Western Blot
Human OVCAR-3; WB Suggested Anti-DNMT3B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: OVCAR-3 cell lysate. DNMT3B is supported by BioGPS gene expression data to be expressed in OVCAR3.; DNMT3B antibody - middle region (ARP49124_P050) in Human OVCAR-3 cells using Western Blot
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence analysis of HeLa cells, using DNMT3B Antibody. The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from HT-29 cells, using DNMT3B Antibody. The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of DNMT3B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of DNMT3B staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of DNMT3B expression in A549 (A); COS7 (B); HeLa (C) whole cell lysates.
Western blot analysis of extracts from HT-29 cells, using DNMT3B antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from HuvEc cells (Lane 2), using DNMT3B Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using DNMT3B antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using DNMT3B antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts of A549 cell line, using DNMT3B antibody.
Sample Type : Lane 1: 20ug mouse mesenchymal stem cell lysate Primary Antibody Dilution : 1:2000 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1:10,000 Color/Signal Descriptions: DNMT3B Gene Name: Anonymous Submitted by:
WB Suggested Anti-DNMT3B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: OVCAR-3 cell lysateDNMT3B is supported by BioGPS gene expression data to be expressed in OVCAR3
Western Blot: Dnmt3b Antibody [TA336294] - Analysis using TA336294 to detect overexpressed DNMT-38 in HCT116 cells. Lane 1: 40 ug of nuclear extract from untransfected T24 degrees Cells. Lane 2-3: 40 ug of nuclear extracts transiently transfected to overe
Immunohistochemistry: Dnmt3b Antibody [TA336294] - Dnmt3b antibody was tested in mouse testis using DAB with hematoxylin counterstain.
Immunocytochemistry/Immunofluorescence: Dnmt3b Antibody [TA336294] - Dnmt3b antibody was tested in NIH-3T3 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Western Blot: Dnmt3b Antibody (52A1018) [TA336379] - Analysis for Dnmt3b at 2 ug/ml against 10 ug of cell lysates of 293 transfected with mouse Dnmt3a (Lanes 1) and Dnmt3b (Lanes 2). A protein band of approximate mol. wt. of 110 kDa is only detected in 2
Western Blot: Dnmt3b Antibody (52A1018) [TA336379] - Analysis of Dnmt3b in A) NIH 3T3 cell lysate, B) recombinant Dnmt3b1 protein (TA336379), and C) 2102Ep human embryonal carcinoma cell lysate using TA336379 at 4 ug/ml, 0.5 ug/ml, and 3 ug/ml respectivel
Immunocytochemistry/Immunofluorescence: Dnmt3b Antibody (52A1018) [TA336379] - Dnmt3b antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody dilution of
Flow Cytometry: Dnmt3b Antibody (52A1018) [TA336379] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Dnmt3b antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was
WB using the antibody against DNMT3B diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of antibody against DNMT3B. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:220,000.
HeLa cells were stained with the antibody against DNMT3B and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the DNMT3B antibody (left) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
WB using the antibody against DNMT3B diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the MW marker (in kDa) is shown on the left.
Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against mouse DNMT3B in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:13,500.
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA313761 | DNMT3B antibody |
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| Rabbit polyclonal anti-DNMT3B antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, ICC/IF, P, WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA327439 | DNMT3B antibody |
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| DNMT3B Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA331002 | DNMT3B antibody |
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| Rabbit polyclonal Anti-DNMT3B Antibody | |||||||
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Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze | WB |
50 µg /
€360.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA336294 | DNMT3B antibody |
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| Rabbit Polyclonal DNMT3B Antibody | |||||||
![Western Blot: Dnmt3b Antibody [TA336294] - Analysis using TA336294 to detect overexpressed DNMT-38 in HCT116 cells. Lane 1: 40 ug of nuclear extract from untransfected T24 degrees Cells. Lane 2-3: 40 ug of nuclear extracts transiently transfected to overe](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB100-266-1-W.jpg) |
Rabbit | Purified | Hu, Ms | C, ICC/IF, IP, P, WB |
0.1 ml /
€416.00
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA347116 | DNMT3B antibody |
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| Rabbit Polyclonal DNMT3B Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E |
50 µg /
€392.00
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA347117 | DNMT3B antibody |
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| Rabbit Polyclonal DNMT3B Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AP01182PU-N | DNMT3B antibody |
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Rabbit | Aff - Purified | Hu | P, WB |
0.1 mg /
€350.00
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| Acris Antibodies GmbH | |||||||
| +1 additional image |
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| AP11052PU-N | DNMT3B antibody |
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Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€370.00
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| Acris Antibodies GmbH | |||||||
| +2 additional images |
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| AP31351PU-N | DNMT3B (1-50) antibody |
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Rabbit | IgG | Purified | Hu | E, ICC/IF, P, WB |
50 µl /
€380.00
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| Acris Antibodies GmbH | |||||||
| +2 additional images |
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| C11809-1 | DNMT3B antibody |
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Rabbit | Aff - Purified | Hu | E, ICC/IF, P, WB |
50 µg /
€230.00
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| Assay Biotech | |||||||
| +2 additional images |
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| C11809-2 | DNMT3B antibody |
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Rabbit | Aff - Purified | Hu | E, ICC/IF, P, WB |
0.1 mg /
€330.00
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| Assay Biotech | |||||||
| +2 additional images |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN221966 | DNMT3B - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
| KN221966BN | DNMT3B - human gene knockout kit via CRISPR |
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Human | mBFP-Neo |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
| KN221966LP | DNMT3B - human gene knockout kit via CRISPR |
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Human | Luciferase-Puro |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
| KN221966RB | DNMT3B - human gene knockout kit via CRISPR |
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Human | RFP-BSD |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP323206 | DNMT3B (transcript variant 1) |
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| Recombinant protein of human DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
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| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY406229 | DNMT3B overexpression lysate |
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0.1 mg /
€495.00
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| OriGene Technologies, Inc. | ||
| LY416333 | DNMT3B overexpression lysate |
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0.1 mg /
€495.00
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| OriGene Technologies, Inc. |
Lysates (Knockout)
| Catalog No. | ||
|---|---|---|
| LC810061 | DNMT3B-knockout Cell lysate |
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0.1 mg /
€495.00
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| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC223206L2V | Lenti ORF particles, DNMT3B (mGFP-tagged) - Human DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B), transcript variant 1, 200 uL, >10^7 TU/mL |
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200 µl /
€1,865.00
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| OriGene Technologies, Inc. | ||
