DNMT3L antibody

Principal name

DNMT3L antibody

Alternative names for DNMT3L antibody

DNA (cytosine-5)-methyltransferase 3-like

SwissProt ID

Q1LZ50 (Rat), Q9CWR8 (Mouse), Q9UJW3 (Human)

Gene ID

29947 (DNMT3L), 54427 (Dnmt3l), 309680

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of DNMT3L antibody

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue (1-6). Studies show that DNMT3L regulates the activity of DNMT3A and DNMT3B and stimulates their catalytic activities (7). DNMT3L has specifically been linked to the process of carcinogenesis, thru its role in nuclear programming (8).

General readings

Holz-Schietinger, C., et al. J. Biol. Chem. 285(38):29091-29100 (2010).
Manderwad, G.P., et al. Arch. Pathol. Lab. Med. 134(8):1193-1196 (2010).
Kim, H., et al. Int. J. Oncol. 36(6):1563-1572 (2010).
Minami, K., et al. Clin. Cancer Res. 16(10):2751-2759 (2010).
Haggarty, P., et al. PLoS ONE 5 (6), E11329 (2010).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DNMT3L (152-164) antibody

  Rabbit polyclonal anti-DNMT3L antibody  
Rabbit anti-DNMT3L was used at a 1:1,500 dilution to detect human DNMT3L by WB after IP using the same antibody.  Transfection of U2OS cells (10cm dish) was accomplished using 3 µg of GAL4-DNMT3L. Protein extraction using IPH at 150mM. For WB the antibody was used at 0.4µg/ml in TBS milk 2%, BSA 0.5%. Detection occurred using ECL.  Rabbit Aff - Purified Hu, Ms E, IP, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

DNMT3L antibody

  Anti-DNMT3L Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human colon and thyroid cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human colon and thyroid cancer tissue using DNMT3L antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNMT3L antibody

  Anti-DNMT3L Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using DNMT3L antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNMT3L (1-387) antibody

  Mouse monoclonal Dnmt3L Antibody  
Western blot analysis of DNMT3L in a human tissue mix using a 1:1000 dilution of the antibody Mouse IgG1 S117-9 Purified Hu C, ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNMT3L antibody

Transfected cell immunofluorescence: COS cells expressing Myc-tagged human DNMT3L. Red = rabbit anti-Myc, Green = N117/9, Blue = Hoechst nuclear stain. Mouse IgG1 N117/9 Supernatant Hu C, ICC/IF, WB
5 ml / €460.00
  Antibodies Incorporated

NeuroMab

DNMT3L antibody

Transfected cell immunofluorescence: COS cells expressing Myc-tagged human DNMT3L. Red = rabbit anti-Myc, Green = N117/9, Blue = Hoechst nuclear stain. Mouse IgG1 N117/9 Purified Hu C, ICC/IF, WB
0.1 ml / €540.00
  Antibodies Incorporated

NeuroMab

DNMT3L (322-337) antibody

Figure 1. Staining DNMT3L in Tonsil by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Purified Hu E, P
50 µg / €470.00
  Acris Antibodies GmbH

DNMT3L (C-term) antibody

AP11060PU-N DNMT3L antibody staining of Formalin-Fixed, Paraffin-Embedded Human cancer tissue using Peroxidase-conjugated secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DNMT3L antibody

Western blot analysis of Mouse Dnmt3L Antibody Cat.-No AP11074PU-N in mouse liver tissue lysates (35ug/lane).This demonstrates the Dnmt3l antibody detected the Dnmt3l protein (arrow). Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DNMT3L (C-term) antibody

Western blot analysis of DNMT3L Antibody (C-term) Cat.-No AP51304PU-N in Jurkat cell line lysates (35ug/lane). This demonstrates the DNMT3L antibody detected the DNMT3L protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

DNMT3L - human gene knockout kit via CRISPR

DNMT3L - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

DNMT3L - human gene knockout kit via CRISPR

DNMT3L - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

DNMT3L - human gene knockout kit via CRISPR

DNMT3L - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

DNMT3L - human gene knockout kit via CRISPR

DNMT3L - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dnmt3l - mouse gene knockout kit via CRISPR

Dnmt3l - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Dnmt3l - mouse gene knockout kit via CRISPR

Dnmt3l - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dnmt3l - mouse gene knockout kit via CRISPR

Dnmt3l - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dnmt3l - mouse gene knockout kit via CRISPR

Dnmt3l - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DNMT3L (transcript variant 2)

  Recombinant protein of human DNA (cytosine-5-)-methyltransferase 3-like (DNMT3L), transcript variant 2  
DNMT3L Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

DNMT3L (full length, C-term DDK tag, transcript variant 1)

  Purified recombinant protein of Human DNA (cytosine-5-)-methyltransferase 3-like (DNMT3L), transcript variant 1, full length, with C-terminal DDK tag, expressed in sf9, 20ug  
DNMT3L Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €399.00
  OriGene Technologies, Inc.

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