DNMT3L antibody

Principal name

DNMT3L antibody

Alternative names for DNMT3L antibody

DNA (cytosine-5)-methyltransferase 3-like

SwissProt ID

Q1LZ50 (Rat), Q9CWR8 (Mouse), Q9UJW3 (Human)

Gene ID

29947 (DNMT3L), 54427 (Dnmt3l)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of DNMT3L antibody

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternative splicing results in two transcript variants. An additional splice variant has been described but its biological validity has not been determined. [provided by RefSeq]. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. Isoform 2 is 1 aa shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Holz-Schietinger, C., et al. J. Biol. Chem. 285(38):29091-29100 (2010).
Manderwad, G.P., et al. Arch. Pathol. Lab. Med. 134(8):1193-1196 (2010).
Kim, H., et al. Int. J. Oncol. 36(6):1563-1572 (2010).
Minami, K., et al. Clin. Cancer Res. 16(10):2751-2759 (2010).
Haggarty, P., et al. PLoS ONE 5 (6), E11329 (2010).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DNMT3L (152-164) antibody

Rabbit anti-DNMT3L was used at a 1:1,500 dilution to detect human DNMT3L by WB after IP using the same antibody.  Transfection of U2OS cells (10cm dish) was accomplished using 3 µg of GAL4-DNMT3L. Protein extraction using IPH at 150mM. For WB the antibody was used at 0.4µg/ml in TBS milk 2%, BSA 0.5%. Detection occurred using ECL.  Rabbit Aff - Purified Hu, Ms E, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNMT3L antibody

Predicted cell location: Cytoplasm. Positive control: Human colon and thyroid cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human colon and thyroid cancer tissue using DNMT3L antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNMT3L antibody

Predicted cell location: Cytoplasm. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using DNMT3L antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNMT3L (1-387) antibody

Western blot analysis of DNMT3L in a human tissue mix using a 1:1000 dilution of the antibody Mouse IgG1 S117-9 Purified Hu C, ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNMT3L antibody

Transfected cell immunofluorescence: COS cells expressing Myc-tagged human DNMT3L. Red = rabbit anti-Myc, Green = N117/9, Blue = Hoechst nuclear stain. Mouse IgG1 N117/9 Supernatant Hu C, ICC/IF, WB
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

DNMT3L antibody

Transfected cell immunofluorescence: COS cells expressing Myc-tagged human DNMT3L. Red = rabbit anti-Myc, Green = N117/9, Blue = Hoechst nuclear stain. Mouse IgG1 N117/9 Purified Hu C, ICC/IF, WB
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

DNMT3L (322-337) antibody

Figure 1. Staining DNMT3L in Tonsil by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Purified Hu E, P
50 µg / €470.00
  Acris Antibodies GmbH

DNMT3L (C-term) antibody

AP11060PU-N DNMT3L antibody staining of Formalin-Fixed, Paraffin-Embedded Human cancer tissue using Peroxidase-conjugated secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DNMT3L antibody

Western blot analysis of Mouse Dnmt3L Antibody Cat.-No AP11074PU-N in mouse liver tissue lysates (35ug/lane).This demonstrates the Dnmt3l antibody detected the Dnmt3l protein (arrow). Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DNMT3L (C-term) antibody

Western blot analysis of DNMT3L Antibody (C-term) Cat.-No AP51304PU-N in Jurkat cell line lysates (35ug/lane). This demonstrates the DNMT3L antibody detected the DNMT3L protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DNMT3L antibody

DNMT3L MaxPab polyclonal antibody. Western Blot analysis of DNMT3L expression in human pancreas. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

DNMT3L antibody

DNMT3L MaxPab rabbit polyclonal antibody. Western Blot analysis of DNMT3L expression in human liver. Rabbit Serum Hu, Ms E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

DNMT3L antibody

DNMT3L MaxPab rabbit polyclonal antibody. Western Blot analysis of DNMT3L expression in mouse liver. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

DNMT3L antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the DNMT3L polyclonal antibody ( Cat # PAB2218 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P
0.4 ml / €390.00
  Abnova Taiwan Corp.

DNMT3L antibody

DNMT3L Rabbit Ms E
0.4 ml / €390.00
  Abnova Taiwan Corp.

DNMT3L antibody

Immunoblotting of DNMT3L polyclonal antibody ( Cat # PAB10043 ) was usedat a 1:1,500 dilution to detect human DNMT3L by western blot after immunoprecipitation using the same antibody.
Transfection of U2-OS cells ( 10cm dish ) was accomplished using 3 µg of GAL4-DNMT3L.
Protein extraction using IPH at 150 mM.
For IP and WB conditions see Fuks et al. ( 2000 ).
For western blotting the antibody was used at 0.4 µg/ml in TBS milk 2%, BSA 0.5%.
Detection occurred using ECL.
Note: this antibody works for western blotting only following immunoprecipitation.
We have not had good success using the antibody directly for western blotting.
The expected molecular weight of human DNMT3L is 43.6 kDa. Hu, Ms
0.1 mg / €560.00
  Abnova Taiwan Corp.

DNMT3L antibody

Western Blot: Dnmt3L Antibody [NB600-484] - NB 600-484   DNMT 3L antibody Rabbit IgG Aff - Purified Hu, Ms E, IP, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

DNMT3L antibody

DNMT3L Mouse IgG1 S117-9 Purified Hu ICC/IF, IP, WB
0.1 mg / €450.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DNMT3L (transcript variant 2)

DNMT3L Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

DNMT3L (full length, C-term DDK tag, transcript variant 1)

DNMT3L Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €399.00
  OriGene Technologies, Inc.

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