DNTT / TDT antibody

Principal name

DNTT / TDT antibody

Alternative names for DNTT / TDT antibody

DNA nucleotidylexotransferase, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Terminal transferase

SwissProt ID

P04053 (Human), P06526 (Bovin), P09838 (Mouse), P36195 (Chick), P42118 (Xenla)

Gene ID

1791 (DNTT), 21673 (Dntt)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Mky (Monkey), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of DNTT / TDT antibody

Terminal Deoxynucleotidyl Transferase (TdT). TdT is a DNA polymerase located in the cell nucleus which catalyses the polymerization of deoxynucleotides at the 3’ hydroxyl ends of oligo or polydeoxynucleotide initiators and functions without a template. The Terminal Deoxynucleotidyl Transferase (TdT) enzyme involved in DNA polymerization and is localized in the nucleus of several hematopoeitic cells such as prothymocytes, precursor T and a subset of B cells. The TdT protein is a 58 kDa nuclear protein. The expression of TdT has been known as a nuclear maker for the hemopeotic cells for many years (1). The conspicuous absence of expression and activity of TdT clearly distinguishes B cell development in the fetus from that in adult bone marrow. The TdT is expressed in most of cases of acute lymphatic leukemia (ALL), and non Hodgkinson lymphoma (NHL), in a subset of chronic and acute myeloid leukemia (CML and AML), acute non-lymphocytic leukemia (ANLL; 1, 2). Detection of nuclear expression of TdT is a valuable method for detection of various type of leukemia and monitoring minimal residual leukemic cells. The DNA polymerase, TdT, is involved in the rearrangement of the T-cell receptor genes in early thymocytes and in rearrangement of of the immunoglobulin genes during B-cell development. These rearrangements of the T-cell receptor genes and the immunoglobulin genes are performed in the nucleus and are lost upon maturation of the immune cells. Detection of TdT by flow cytometry and by IHC can be useful in detecting a small percentage of leukemic cells, in cases such as minimal residual T-cell acute lymphoblastic leukemia (3). Other antibodies that are used to detect residual T-cell ALL are combination of Anti-TdT with CD1a, CD2, CD5 and CD7 antibodies. The sensitivity of detecting smaller percentage of cell expressing TdT can be enhanced by double or triple staining with Anti-TdT and a combination of anti-CD antibodies. Antibodies used for double staining of AML cells are combination of Anti-TdT and Anti-HLA-DR, CD13, CD33, CD34 and CD45 antibodies.

General readings

Kubota,T., (2007) Genes Cells 12 (8), 941-959

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DNTT / TDT antibody

  Rabbit Polyclonal TdT Antibody  
Western Blot: TdT Antibody [TA337211] - WB detection of TDT protein in (A) lysate of MOLT4 human leukemia cell line and (B) partial recombinant protein by using TDT antibody. Primary antibody concentration used: 1 ug/ml for Molt4 lysate, 0.05 ug/ml for re Rabbit Purified Hu C, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNTT / TDT antibody

  Rabbit Polyclonal TdT Antibody  
Immunohistochemistry staining of Paraffin Thymus tissue by TdT antibody (dilution: 1:100 -1:500; visualization of staining: Nuclear) Rabbit Purified Hu P
1 ml / €800.00
  OriGene Technologies, Inc.

DNTT / TDT antibody

  Mouse Monoclonal anti-TdT Antibody  
A20 mouse B cell lymphoma cell extracts (Lane 1) and Jurkat cell extracts (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-mouse TdT antibody (clone L10-2). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection. Mouse IgG2b L10-2 Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNTT / TDT antibody

  Rabbit Polyclonal Anti-DNTT Antibody  
WB Suggested Anti-DNTT Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

DNTT / TDT antibody

  Rabbit Polyclonal Anti-DNTT Antibody  
WB Suggested Anti-DNTT Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

DNTT / TDT antibody

  Mouse Monoclonal anti-TdT Antibody  
Western blot analysis of mouse thymus (lane 1) cell, Jurkat (lane 2) and Daudi (lane 3) cells using anti-TdT antibody (clone 7H9A33). Mouse 7H9A33 Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DNTT / TDT antibody

  Mouse Monoclonal anti-TdT Antibody  
A20 mouse B cell lymphoma cell extracts (Lane 1) and Jurkat cell extracts (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-mouse TdT antibody (clone L10-2). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection. Mouse L10-2 Purified Hu, Ms WB
25 µg / €248.00
  OriGene Technologies, Inc.

DNTT / TDT antibody

  Rabbit Polyclonal anti-TdT Antibody  
DNTT / TDT Rabbit Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNTT / TDT antibody

  Rabbit Monoclonal anti-Terminal Deoxynucleotidyl Transferase (TdT) Antibody  
DNTT / TDT Rabbit SP150 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DNTT / TDT antibody

Formalin-Fixed, Paraffin-Embedded Human thymus stained with TDT antibody Cat.-No. AM08217SU using peroxidase-conjugate and DAB chromogen. Note nuclear staining of thymocytes. Mouse IgG2a SEN28 Supernatant Hu C, P
1 ml / €780.00
  Acris Antibodies GmbH

DNTT / TDT antibody

Formalin-Fixed, Paraffin-Embedded Human thymus stained with TDT antibody Cat.-No. AM08217SU using peroxidase-conjugate and DAB chromogen. Note nuclear staining of thymocytes. Mouse IgG2a SEN28 Supernatant Hu C, P
0.5 ml / €500.00
  Acris Antibodies GmbH

DNTT / TDT (C-term) antibody

Western blot with TDT Polyclonal Antibody Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

DNTT / TDT (C-term) antibody

TdT Antibody (C-term) (AP54213PU-N) western blot analysis in K562 cell line lysates (35 µg/lane). This demonstrates the TdT antibody detected the TdT protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

DNTT - human gene knockout kit via CRISPR

DNTT - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

DNTT - human gene knockout kit via CRISPR

DNTT - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

DNTT - human gene knockout kit via CRISPR

DNTT - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

DNTT - human gene knockout kit via CRISPR

DNTT - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dntt - mouse gene knockout kit via CRISPR

Dntt - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Dntt - mouse gene knockout kit via CRISPR

Dntt - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dntt - mouse gene knockout kit via CRISPR

Dntt - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

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