DOT1L antibody

Principal name

DOT1L antibody

Alternative names for DOT1L antibody

Histone H3-K79 methyltransferase, H3-K79-HMTase, DOT1-like protein, Lysine N-methyltransferase 4, KIAA1814, KMT4, Histone-lysine N-methyltransferase H3 lysine-79 specific

SwissProt ID

Q8TEK3 (Human)

Gene ID

84444 (DOT1L)

Available reactivities

Hu (Human)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP)

Background of DOT1L antibody

The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is ictive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011].

20 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DOT1L antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI2B10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DOT1L antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI5D12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DOT1L antibody

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DOT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA802482) Mouse IgG1 OTI1D8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DOT1L antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI1F6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DOT1L antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI1F8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DOT1L antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DOT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802738) Mouse IgG2b OTI6E7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DOT1L antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI8A11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DOT1L antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI3E9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DOT1L antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI11H2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DOT1L antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DOT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802742) Mouse IgG1 OTI1F1D4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DOT1L antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI7H6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DOT1L antibody

DOT1L Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

DOT1L antibody

DOT1L Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

DOT1L antibody

Detection limit for recombinant GST tagged DOT1L is approximately 0.03ng/ml as a capture antibody. Mouse IgG1 6A6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DOT1L antibody

DOT1L Mouse IgG1 8B2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DOT1L antibody

Immunohistochemistry-Paraffin: Dot1L Antibody [NB100-40845] - Please see notes section Rabbit Aff - Purified Hu ICC/IF, IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

DOT1L antibody

Western Blot: DOT1L Antibody [NB100-40846] - Detection of Human DOT1L by Western Blot of Immunoprecipitates.  Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DOT1L antibody NB100-40846 used for IP at 3 mcg/mg lysate. DOT1L was also immunoprecipitated by rabbit anti- DOT1L antibody NB100-40845, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time 10 seconds. Rabbit Aff - Purified Hu IP
0.1 mg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DOT1L

DOT1L Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

DOT1L

DOT1L Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

DOT1L overexpression lysate

DOT1L overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

20 Item(s)

per page
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