DOT1L antibody

Principal name

DOT1L antibody

Alternative names for DOT1L antibody

Histone H3-K79 methyltransferase, H3-K79-HMTase, DOT1-like protein, Lysine N-methyltransferase 4, KIAA1814, KMT4, Histone-lysine N-methyltransferase H3 lysine-79 specific

SwissProt ID

Q8TEK3 (Human)

Gene ID

84444 (DOT1L)

Available reactivities

Hu (Human)

Available hosts

Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of DOT1L antibody

Parental cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The KO cell lysates are the cell homogenate in RIPA buffer made from the KO cell lines. The protein concentration was determined with BCA assay. A vial of lysate from the parental cell line was also included as an internal control. To facilitate transportation and protein, the products are supplied as lyophilized proteins.

Items 1 to 20 of 21 total

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DOT1L antibody

  DOT1L mouse monoclonal antibody, clone OTI2B10 (formerly 2B10)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI2B10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DOT1L antibody

  DOT1L mouse monoclonal antibody, clone OTI5D12 (formerly 5D12)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI5D12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DOT1L antibody

  DOT1L mouse monoclonal antibody, clone OTI1D8 (formerly 1D8)  
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DOT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA802482) Mouse IgG1 OTI1D8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DOT1L antibody

  DOT1L mouse monoclonal antibody, clone OTI1F6 (formerly 1F6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI1F6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DOT1L antibody

  DOT1L mouse monoclonal antibody, clone OTI1F8 (formerly 1F8)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI1F8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DOT1L antibody

  DOT1L mouse monoclonal antibody, clone OTI6E7 (formerly 6E7)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DOT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802738) Mouse IgG2b OTI6E7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DOT1L antibody

  DOT1L mouse monoclonal antibody, clone OTI8A11 (formerly 8A11)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI8A11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DOT1L antibody

  DOT1L mouse monoclonal antibody, clone OTI3E9 (formerly 3E9)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI3E9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DOT1L antibody

  DOT1L mouse monoclonal antibody, clone OTI11H2 (formerly 11H2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI11H2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DOT1L antibody

  DOT1L mouse monoclonal antibody, clone OTI1F1D4 (formerly 1F1D4)  
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DOT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802742) Mouse IgG1 OTI1F1D4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DOT1L antibody

  DOT1L mouse monoclonal antibody, clone OTI7H6 (formerly 7H6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Mouse IgG1 OTI7H6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DOT1L antibody

DOT1L Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

DOT1L antibody

DOT1L Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

DOT1L antibody

Detection limit for recombinant GST tagged DOT1L is approximately 0.03ng/ml as a capture antibody. Mouse IgG1 6A6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DOT1L antibody

DOT1L Mouse IgG1 8B2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DOT1L

DOT1L Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

DOT1L

DOT1L Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

DOT1L overexpression lysate

DOT1L overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Lysates (Knockout)

Catalog No.    

DOT1L-knockout Cell lysate

DOT1L-knockout Cell lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, DOT1L (mGFP-tagged)-Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L), 200 uL, >10^7 TU/mL

Lenti ORF particles, DOT1L (mGFP-tagged)-Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L), 200 uL, >10^7 TU/mL  
200 µl / €2,530.00
  OriGene Technologies, Inc.

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