Doublecortin antibody

Principal name

Doublecortin antibody

Alternative names for Doublecortin antibody

Lissencephalin-X, Doublin, DCX, DBCN, LISX, Neuronal migration protein doublecortin

SwissProt ID

O43602 (Human), O88809 (Mouse), Q9ESI7 (Rat)

Gene ID

1641 (DCX), 13193 (Dcx)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Bov (Bovine), Can (Canine), Por (Porcine), Eq (Equine), Prim (Primate), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat, Chicken

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C), Flow Cytometry (F)

Background of Doublecortin antibody

Doublecortin was origilly discovered since defects in the gene encoding it are causative of X-linked lissencephaly, a rare group of brain malformations resulting in a smooth cerebral cortex caused by aberrant neurol migration during development. The me Doublecortin comes from the unusual layering of the cortex in this form of lissencephaly, which appears to have a second deep cortical layer of neurons. This layer consists of neurons which did not migrate from the subventricular zone to the normal cortical layer. Patients with this defect suffer from seizures and mental retardation. The HGNC me for Doublecortin is DCX, and it is also known as Doublin, Lissencephalin-X, DBCN and Lis-X. Four proteins encoded by the DCX produce bands of about 35kDa and 45kDa on Western blots. The 45kDa form is known as Lis-XA while the smaller forms are generated by alterte transcription, are all missing the first 81 amino acids of Lis-XA, and are referred to as Lis-XB, Lis-XC, Lis-XD. There are minor amino acid sequence differences between these three smaller isoforms. All of these protein contain two so-called Doublecortin domains, each about 90 amino acids long, which are believed to function in binding to microtubules, a C-termil serine and proline rich region which may become phosphorylated in vivo. The doublecortin protein appears to function as a microtubule and actin binding protein and may interact with Lis-1, a member of the beta-transducin or WD protein family, a protein mutations of which are also associated with lissencephaly. DCX is expressed very early in neurol development, as neuroblasts become post-mitotic, but is lost as neurons mature. Developing neurons start to lose DCX expression about the time that they begin to express NeuN, a neurol specific protein characteristic of more mature neurons, now known to correspond to the R binding protein Fox3. Antibodies to DCX are used to identify stem cells in sections and in tissue culture, and to see if neurogenesis is taking place.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Doublecortin antibody


Doublecortin antibody Cat.-No AP32017PU-N staining of Mouse fetal Brain lysate at 0.5 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Doublecortin (232-242) antibody

(0.01ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Doublecortin antibody

Western blot analysis of extracts from Jurkat cells, using Doublecortin (Ab-376) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Doublecortin antibody

Western blot analysis of extracts from RAW264.7cells, using Doublecortin (Ab-297) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Doublecortin antibody

Predicted band size: 45 kDa . Positive control: Molt-4 and Hela cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2: Molt-4 and Hela cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Doublecortin antibody

Western Blot: Doublecortin Antibody (3E1) [TA336855] - Western blot of crude rat brain extract from a postnatal 3 day animal stained with TA336855. Two bands at ~45kDa and ~35kDa show that TA336855 binds to an epitope in the region of Doublecortin shared Mouse IgG2a 3E1 Purified Bov, Hu, Ms, Por, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Doublecortin antibody

WB Suggested Anti-DCX Antibody Titration: 0.2-1 ug/ml; Positive Control: 293T cell lysate Rabbit IgG Purified Can, Eq, Hu, Ms, Por WB
50 µg / €325.00
  OriGene Technologies, Inc.

Doublecortin antibody

WB Suggested Anti-DCX Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Doublecortin antibody

Anti-DCX mouse monoclonal antibody (TA501778) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DCX(RC221891). Mouse IgG1 OTI4A3 Purified Hu F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Doublecortin antibody

Immunofluorescence of cultured rat neurons showing strong cytoplasmic staining of Doublecortin (green) in developing neurons and GFAP (Cat.-No AP08674SU-N) in red. Mouse IgG2a 3E1 Aff - Purified Bov, Hu, Ms, Rt ICC/IF, WB
0.1 ml / €380.00
  Acris Antibodies GmbH

Doublecortin antibody

Immunohistochemistry analysis of Doublecortin antibody (Cat.-No.: AP06599PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Doublecortin antibody

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with DCX Antibody (Cat.#AP12410PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Doublecortin antibody

Doublecortin staining of cochlear neurons in the cochlear ganglion of an adult Mouse brain (green), reed staining is neurofilament immunoreactivity, as seen using Rabbit antibody. Chicken Ig Purified Hu, Ms C, ICC/IF
1.0 ml / €440.00
  Acris Antibodies GmbH

Doublecortin antibody

Western Blot analysis of DCX expression in transfected 293T cell line (H00001641-T01) by DCX MaxPab polyclonal antibody.

Lane 1: DCX transfected lysate(39.6 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Doublecortin antibody

Immunofluorescence of monoclonal antibody to DCX on HeLa cell . [antibody concentration 10 ug/ml] Mouse IgG1 1G12 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Doublecortin antibody

Doublecortin Mouse IgG1 3B7 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

Doublecortin pSer376 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using Doublecortin (Phospho-Ser376) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
50 µg / €205.00
  Assay Biotech

Doublecortin pSer376 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using Doublecortin (Phospho-Ser376) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €310.00
  Assay Biotech

Doublecortin antibody

Western blot analysis of extracts from RAW264.7 cells, using Doublecortin (Ab-297) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

Doublecortin antibody

Western blot analysis of extracts from RAW264.7 cells, using Doublecortin (Ab-297) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

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