DPH1 / OVAC1 antibody

Principal name

DPH1 / OVAC1 antibody

Alternative names for DPH1 / OVAC1 antibody

DPH2L, DPH2L1, Diphthamide biosynthesis protein 1, DPH1 homolog, Diphthamide biosynthesis protein 2 homolog-like 1, Ovarian cancer-associated gene 1 protein

SwissProt ID

Q3SYT1 (Bovin), Q5NCQ5 (Mouse), Q5ZHX9 (Chick), Q9BZG8 (Human)

Gene ID

1801 (DPH1), 116905 (Dph1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ye (Yeast)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of DPH1 / OVAC1 antibody

Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]). Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).[supplied by OMIM]. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-40 AF321876.1 1-40 41-1028 BC003099.1 3-990 1029-1410 BP417755.1 78-459 1411-2188 AK090530.1 1484-2261 2189-2200 AF321876.1 2189-2200

General readings

Liu, S., et al. Mol. Cell. Biol. 24(21):9487-9497 (2004).
Cardoso, C., et al. Am. J. Hum. Genet. 72(4):918-930 (2003).
Chen, C.M., et al. Biochem. Biophys. Res. Commun. 286(5):1019-1026 (2001).
Salicioni, A.M., et al. Genomics 69(1):54-62 (2000).
Bruening, W., et al. Cancer Res. 59(19):4973-4983 (1999).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DPH1 / OVAC1 antibody

  Rabbit Polyclonal Anti-DPH1 Antibody  
WB Suggested Anti-DPH1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: RPMI 8226 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

DPH1 / OVAC1 antibody

  Rabbit Polyclonal Anti-DPH1 Antibody  
WB Suggested Anti-DPH1 Antibody Titration: 0.2-1 ug/ml; Positive Control: MCF7 cell lysate DPH1 is supported by BioGPS gene expression data to be expressed in MCF7 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ye, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

DPH1 / OVAC1 (C-term) antibody

Western blot analysis of DPH1 / OVAC1 Antibody (C-term) Cat.-No AP51311PU-N in A375 cell line lysates (35ug/lane). This demonstrates the DPH1 antibody detected the DPH1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

DPH1 - human gene knockout kit via CRISPR

DPH1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

DPH1 - human gene knockout kit via CRISPR

DPH1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

DPH1 - human gene knockout kit via CRISPR

DPH1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

DPH1 - human gene knockout kit via CRISPR

DPH1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dph1 - mouse gene knockout kit via CRISPR

Dph1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Dph1 - mouse gene knockout kit via CRISPR

Dph1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dph1 - mouse gene knockout kit via CRISPR

Dph1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dph1 - mouse gene knockout kit via CRISPR

Dph1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DPH1 / OVAC1

  Recombinant protein of human DPH1 homolog (S. cerevisiae) (DPH1)  
DPH1 / OVAC1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, DPH1 (mGFP-tagged) - Human DPH1 homolog (S. cerevisiae) (DPH1), 200 uL, >10^7 TU/mL

Lenti ORF particles, DPH1 (mGFP-tagged) - Human DPH1 homolog (S. cerevisiae) (DPH1), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, DPH1 (Myc-DDK tagged) - Human DPH1 homolog (S. cerevisiae) (DPH1) , 200 uL, >10^7 TU/mL

Lenti ORF particles, DPH1 (Myc-DDK tagged) - Human DPH1 homolog (S. cerevisiae) (DPH1) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

14 Item(s)

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