DPH1 / OVAC1 antibody

Principal name

DPH1 / OVAC1 antibody

Alternative names for DPH1 / OVAC1 antibody

DPH2L, DPH2L1, Diphthamide biosynthesis protein 1, DPH1 homolog, Diphthamide biosynthesis protein 2 homolog-like 1, Ovarian cancer-associated gene 1 protein

SwissProt ID

Q3SYT1 (Bovin), Q5NCQ5 (Mouse), Q5ZHX9 (Chick), Q9BZG8 (Human)

Gene ID

1801 (DPH1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ye (Yeast)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of DPH1 / OVAC1 antibody

Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]). Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).[supplied by OMIM]. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-40 AF321876.1 1-40 41-1028 BC003099.1 3-990 1029-1410 BP417755.1 78-459 1411-2188 AK090530.1 1484-2261 2189-2200 AF321876.1 2189-2200

General readings

Liu, S., et al. Mol. Cell. Biol. 24(21):9487-9497 (2004).
Cardoso, C., et al. Am. J. Hum. Genet. 72(4):918-930 (2003).
Chen, C.M., et al. Biochem. Biophys. Res. Commun. 286(5):1019-1026 (2001).
Salicioni, A.M., et al. Genomics 69(1):54-62 (2000).
Bruening, W., et al. Cancer Res. 59(19):4973-4983 (1999).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DPH1 / OVAC1 antibody

WB Suggested Anti-DPH1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: RPMI 8226 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

DPH1 / OVAC1 antibody

WB Suggested Anti-DPH1 Antibody Titration: 0.2-1 ug/ml; Positive Control: MCF7 cell lysate DPH1 is supported by BioGPS gene expression data to be expressed in MCF7 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ye, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

DPH1 / OVAC1 (C-term) antibody

Western blot analysis of DPH1 / OVAC1 Antibody (C-term) Cat.-No AP51311PU-N in A375 cell line lysates (35ug/lane). This demonstrates the DPH1 antibody detected the DPH1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DPH1 / OVAC1 antibody

Immunofluorescence of monoclonal antibody to DPH1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2b 2C5 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DPH1 / OVAC1 antibody

DPH1 / OVAC1 Rabbit Hu WB
0.1 ml / €560.00
  Abnova Taiwan Corp.

DPH1 / OVAC1 antibody

DPH1 / OVAC1 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

DPH1 / OVAC1 antibody

Human Adult Liver; Rabbit Anti-DPH1 Antibody . Catalog Number: ARP51956_P050. Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver . Observed Staining: Cytoplasm (perinuclear) but not nuclear in hepatocytes, strong signal, low tissue distribution. Primary Antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification: 20X. Exposure Time: 0.5 – 2.0 sec. Protocol located in Reviews and Data.; DPH1 antibody - middle region (ARP51956_P050) in Human Adult Liver cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

DPH1 / OVAC1 antibody

DPH1 / OVAC1 Rabbit Serum Hu WB
0.1 ml / €400.00
  Immuquest

DPH1 / OVAC1 antibody

DPH1 / OVAC1 Rabbit Serum Hu WB
50 µl / €360.00
  Immuquest

DPH1 / OVAC1 antibody

DPH1 / OVAC1 Rabbit IgG Hu WB
0.1 ml / €340.00
  Novus Biologicals Inc.

DPH1 / OVAC1 antibody

Western Blot: OVCA1 Antibody [NBP1-56693] - This Anti-DPH1 antibody was used in Western Blot of MCF7 tissue lysate at a concentration of 0.5ug/ml. Rabbit Aff - Purified Hu, Ms WB
50 µg / €440.00
  Novus Biologicals Inc.

DPH1 / OVAC1 antibody

Western Blot: OVCA1 Antibody [NBP1-57711] - This Anti-DPH1 antibody was used in Western Blot of RPMI-8226       tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DPH1 / OVAC1

DPH1 / OVAC1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

DPH1 / OVAC1

DPH1 / OVAC1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

DPH1 / OVAC1

DPH1 / OVAC1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

DPH1 / OVAC1

DPH1 / OVAC1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

DPH1 / OVAC1

DPH1 / OVAC1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

17 Item(s)

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